Journal: Drug Invention Today

Article Id: JPRS-CA-00004565
Title: Estimation of E-guggulsterone, Z-guggulsterone and myrcene in Mahayograj Guggul tablet by high- performance thin-layer chromatography (HPTLC) method: A marketed herbal formulation
Category: Compounds ( Natual and Synthetic ) Analysis
Section: Research Article
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    Objective: Mahayograj Guggul is a herbal formulation, which contains part of Commiphora mukul, the main active constituent is E- and Z-guggulsterone, used for the treatment of Arthritis and cholesterol-lowering activity. Method: Quantification of phytochemical compounds by HPTLC method, this is method is simple, accurate and precise method estimation of Mahayograj Guggul herbal formulation by high-performance thin-layer chromatography (HPTLC) method. Respective standards were spotted on the silica plates for the development process Acetone:Toluene (19:1 v/v)used as mobile phase. The analysis was carried out in the absorbance mode at 254 nm using HPTLC scanner. Results and Discussion: The Retention factor (Rf) values of herbal formulation compared with the Rf value of standard markers such as E-guggulsterone and Z-guggulsterone and myrcene, and it shows confirmation of abovementioned phytochemical compounds. The peak area of herbal formulation and standard compounds was compared to ensure the presence and their quantities were estimated. Results show that this method is a simple, linear, and specific method for the estimation of phytochemical compounds in the marketed herbal formulation by HPTLC techniques. This method can be used for the quantification of guggulsterone isomers as well as myrcene in the marketed herbal formulation.

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    Author(s) Name:

    Sarvesh Sabarathinam, T. M. Vijayakumar*

    Affiliation(s) Name:

    Department of Pharmacy Practice, SRM College of Pharmacy, SRM Institute of Science and Technology, Kancheepuram, Tamil Nadu, India

    *Corresponding author: T. M. Vijayakumar, Department of Pharmacy Practice, SRM College of Pharmacy, SRM Institute of Science and Technology, Kancheepuram, Tamil Nadu, India

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    Author:

    Sarvesh Sabarathinam, T. M. Vijayakumar*

    Title:Estimation of E-guggulsterone, Z-guggulsterone and myrcene in Mahayograj Guggul tablet by high- performance thin-layer chromatography (HPTLC) method: A marketed herbal formulation
    Journal:Drug Invention Today
    Vol(issue):11 (December [ Special Issue 2])
    Year:2019
    Page No: (270-273)
  • Experimental Methods Keywords

    Methodology:Development, High-performance thin-layer chromatography, Linearity, Validation
    Research Materials: Guggulsterone,Myrcene,

Keywords

Development Guggulsterone High-performance thin-layer chromatography Linearity Myrcene Validation

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