Journal: Drug Invention Today

Article Id: JPRS-MSNS-00004595
Title: Prevalence of aminoglycoside modifying enzyme genes in Pseudomonas aeruginosa isolated from Burn Centers in Iraq
Category: Microbiological Study (on Natural/ Synthetic drug)
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Background: Hospitalized burned patients are at high risk to multidrug-resistant infections mainly due to a disruption of the skin barrier, alterations in the specific and nonspecific components of the immune system, and prolong treatment. Pseudomonas aeruginosa is one of the most important causes of healthcare-acquired infections responsible for significant morbidity and mortality in burned patients worldwide. Few studies have handled bacterial isolation from bedsheets for wound burn patients at the Al-Diwaniya Burn Center, Iraq. Methods: A total of 100 swabs from bedsheets of wound burn inpatients at the Al-Diwaniya Burns Center were included in this study. Swab samples were cultured on cetrimide agar. Colonies that were suspected to be P. aeruginosa were confirmed using biochemical tests and analytical profile index 20NE. The study includes molecular detection of three aminoglycosides modifying enzymes genes: acc(3’)-I, aph(3’)-IIb, and aad A1 by polymerase chain reaction (PCR). Results: Throughout this study, among 100 swab samples collected from bedsheets of burn patients, 27% of them yielded bacterial growth P. aeruginosa was most frequently isolated bacterial species (20 isolates). Thirteen isolates (65%) revealed high levels of resistance against aminoglycosides using disk diffusion and minimum inhibitory concentration (MIC) test. The highest MIC range (512–1024 μg/ml) was detected among tested bacterial isolate against gentamycin (55%) and kanamycin (35%), while two isolates (15%) were resistant to amikacin (MIC: 32–128 μg/ml). Multiplex PCR was performed to detect the presence of aminoglycoside modifying enzyme (AME) genes and the results of molecular detection found that aac(3’)-I gene was the most frequently found among P. aeruginosa isolates (46%) either individually (23%) or in combination: acc(3’)-I+ Aph(3’)IIb (15%) and acc(3’)-I+ aadAI (7.7%), whereas one isolate was harboring aadAI gene individually. Conclusion: The current study concludes occurrence of aminoglycosides modifying enzymes harboring. P. aeruginosa that contaminates bedsheets of hospitalized burned patient and high potential of horizontal transfer of resistance genes among bacteria is a growing threat in the burn center and represents an alarm of crisis for clinically effective antipseudomonal aminoglycosides available in burn center.

  • Abstract Audio

    No Audio file found

  • About the authors and Affiliations

    Author(s) Name:

    Majida M. Meteab Alshammari1*, Miaad K. Alkhudhairy2, Ahlam Ali Soghi3

    Affiliation(s) Name:

    1Department of Microbiology, College of Medicine, Jabir Ibn Hayyan Medical University, Najaf, Iraq

    2Department of Community Health, College of Health and Medical Techniques, Al-Furat Al- Awsat Technical University, Najaf, Iraq

    3Department of Biological Science, College of Education, University of Al-Qadisiya, Iraq.

    *Corresponding author: Majida M. Meteab Alshammari, Department of Microbiology, College of Medicine, Jabir Ibn Hayyan Medical University, Najaf, Iraq.

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    0 View More
  • How to Cite my Article.

    Author:

    Majida M. Meteab Alshammari1*, Miaad K. Alkhudhairy2, Ahlam Ali Soghi3

    Title:Prevalence of aminoglycoside modifying enzyme genes in Pseudomonas aeruginosa isolated from Burn Centers in Iraq
    Journal:Drug Invention Today
    Vol(issue):11 (December )
    Year:2019
    Page No: (3149-3154)
  • Experimental Methods Keywords

    Methodology: aadAI, acc(3’)-I, Aminoglycosides Modifying genes, Aminoglycosides resistance, Aph(3’)IIb, Polymerase chain reaction, Pseudomonas aeruginosa
    Research Materials:Hospitalized burned patients

Keywords

aadAI acc(3’)-I Aminoglycosides Modifying genes Aminoglycosides resistance Aph(3’)IIb Polymerase chain reaction Pseudomonas aeruginosa

Our Services

Most Downloaded List