Journal: Drug Invention Today

Article Id: JPRS-P'Col-00004802
Title: Effect of beta-sitosterol on carbohydrate metabolic enzymes in liver of high-fat diet and sucrose-induced diabetic rats
Category: Pharmacology
Section: Research Article
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    Aim: The study was aimed at assessing the effect of β-sitosterol on carbohydrate metabolic enzymes in high-fat diet and sucrose-induced type-2 diabetic rats. Experimental design: Adult male Wistar rats were used and divided into four groups: Group I: Control; Group II: Type-2 diabetic rats; Group III: Type-2 diabetic rats treated with β-sitosterol (20 mg/kg b.wt/day, orally for 30 days); and Group IV: Type-2 diabetic rats treated with metformin (50 mg/kg b.wt/day, orally for 30 days). After 30 days of treatment, animals were anesthetized; liver tissues were dissected out and used for the assessment of glucokinase, glucose-6-phosphatase, glycogen synthase, glycogen phosphorylase, and phosphoenolpyruvate carboxykinase (PEPCK) enzyme activities which were studied by spectrophotometric analysis. Results: The activity of glucose-6-phosphatase, glycogen phosphorylase, and PEPCK was significantly raised (P < 0.05) in type-2 diabetic rats. Conversely, glucokinase and glycogen synthase activities were significantly reduced (P < 0.05) in type-2 diabetic rats. Oral administration of the plant sterol (β-sitosterol) could effectively normalize the detrimental changes caused by high diet induction to that of the control and standard drug metformin. Conclusion: The present study shows that β-sitosterol regulates carbohydrate metabolic enzymes in the liver, thereby β-sitosterol may have therapeutic potential candidate for the management of type-2 diabetes.

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    Author(s) Name:

    R. Saravanan1,2, R. Ponnulakshmi3, D. Anandhi1, G. Valli4, S. Girija5, K. Revathy3, G. Ramajayam6, Selvaraj Jayaraman7*

    Affiliation(s) Name:

    1Central Research Laboratory, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu, India.

    2 Department of Biochemistry, Karpaga Vinayaga Institute of Dental Science, Chinnakolampakkam, Chengalpat District-603308, Tamil Nadu, India.

    3Central Research Laboratory, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu, India.

    4Department of Pharmacology, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu, India.

    5Department of Anatomy, Karpaga Vinayaga Institute of Medical and Technical Sciences Research Centre, Chennai, Tamil Nadu, India.

    6Multi Disciplinary Research Unit, Madurai Medical College, Madurai, Tamil Nadu, India.

    7Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: Dr. Selvaraj Jayaraman, Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

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    Author:

    R. Saravanan1,2, R. Ponnulakshmi3, D. Anandhi1, G. Valli4, S. Girija5, K. Revathy3, G. Ramajayam6, Selvaraj Jayaraman7*

    Title:Effect of beta-sitosterol on carbohydrate metabolic enzymes in liver of high-fat diet and sucrose-induced diabetic rats
    Journal:Drug Invention Today
    Vol(issue):13 (February )
    Year:2020
    Page No: (242-246)
  • Experimental Methods Keywords

    Methodology: spectrophotometric analysis
    Research Materials:beta-sitosterol

Keywords

Beta-sitosterol High-fat diet Phosphoenolpyruvate carboxykinase Glycogen synthase Type-2 diabetes

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