Journal: Drug Invention Today

Article Id: JPRS-MSNS-00004895
Title: Antibacterial activities of actinomycetes isolated from rhizosphere Diospyros celebica Bakh. in Lore Lindu National Park, Indonesia
Category: Microbiological Study (on Natural/ Synthetic drug)
Section: Research Article
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    Aim: The purpose of this research was to isolate, assay, and identify actinomycetes associated with the rhizosphere of ebony plants that produce antibacterial compounds against human pathogens. Materials and Methods: The research included the isolation of actinomycetes using selective Starch Casein Agar (SCA) medium and an assay of the activity of actinomycetes against human pathogenic bacteria – Staphylococcus aureus, Methicillin-resistant S. aureus (MRSA), enteropathogenic Escherichia coli (EPEC), and Vibrio cholerae. The potential isolates of actinomycetes were identified based on 16S rDNA sequence similarity. Results: Seventeen isolates of actinomycetes in the ebony plant rhizosphere were found to produce antibacterial compounds against human pathogens. Among the 17 isolates of actinomycetes, the E512 isolate had the highest activity of extracellular antibacterial compounds that inhibit the growth of V. cholerae, EPEC, MRSA, and S. aureus, with an inhibition zone diameter of 18.55 ± 0.46 mm, 18.73 ± 1.02 mm, 19.81 ± 0.47 mm, and 20.06 ± 0.75 mm, respectively. The E512 isolate had 99% similarity of 16S rDNA sequence with Streptomyces globisporus KCTC 9026. Conclusion: This E512 isolate from the ebony plants’ rhizosphere can be developed as antibiotic-producing actinomycetes, which contribute to human health.

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    Author(s) Name:

    Muhammad Alwi 1,2 *, Suharjono Suharjono3 , Tri Ardyati 3 , Subandi Subandi 4

    Affiliation(s) Name:

    1 Doctoral Program in Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia,

    2 Department of Biology, Faculty of Mathematics and Natural Sciences, University of Tadulako, Palu-Central Sulawesi, Indonesia,

    3 Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia,

    4 Department of Chemistry, Faculty of Mathematics and Natural Sciences, State University of Malang, Indonesia

    *Corresponding author: Muhammad Alwi, Doctoral Program in Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia.

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    Author:

    Muhammad Alwi 1,2 *, Suharjono Suharjono3 , Tri Ardyati 3 , Subandi Subandi 4

    Title:Antibacterial activities of actinomycetes isolated from rhizosphere Diospyros celebica Bakh. in Lore Lindu National Park, Indonesia
    Journal:Drug Invention Today
    Vol(issue):11 (December )
    Year:2019
    Page No: (3400-3408)
  • Experimental Methods Keywords

    Methodology:Antibacterial activities
    Research Materials: Actinomycetes, rhizosphere Diospyros celebica Bakh

Keywords

16S rDNA Actinomycetes Ebony rhizosphere Isolation Pathogenic bacteria

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