Journal: Drug Invention Today

Article Id: JPRS-P'Col-00005080
Title: Evaluation of antibacterial potential of fruiting body extracts of Pleurotus ostreatus in vitro and in vivo study
Category: Pharmacology
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Background: The recent development of bacterium resistant strains has raised worry in health care settings in public of Iraq. This has led to raised research interest in mushrooms. The present research explores the medical chance of the cultivated mushroom oyster mushroom (Pleurotus ostreatus) as a hopeful antibacterial agent against divers’ clinical pathogens that are multidrug resistance (MDR) agents and methicillin-resistant Staphylococcus aureus (MRSA) to present-generation antibiotics. Materials and Methods: Samples enrolled in this study is mushroom belongs to the genus P. ostreatus, obtained from Unit of fungal production/Unit of medicinal and Aromatic plants, College of Agriculture, University of Baghdad, Iraq with sample Code no MF0657151, recorded in NCBI (National center for Biotechnology Information/Part of (US National Institute of Health). S. aureus and Acinetobacter baumannii were already isolated and diagnosed from previous study and preserved in the lab. General method of extraction from the powder of P. ostreatus fruiting bodies was done by three solvents sequentially (chloroform, methanol, and aqueous) solutions. The antibacterial activity was assessed using agar well diffusion procedure; 200 mg/ml from each crude extract have been used. The antimicrobial activity and minimum inhibitory concentration (MIC) were assessed for the methanol crude extract for the potent solution among the others in the ranging from 100, 50, 25, and 12.5 mg/ml. Murine model was used to assess the antibacterial activity of methanol crude extract after cutaneous induced infection of each of clinical pathogen, A. baumannii (MDR), and MRSA. Results: It was seen that the best extract was this belongs methanol. The most potent antibacterial activity of the methanol crude extract was seen within the range of 100 mg/ml–12.5 mg/ml concentrations. Methanol crude extract exhibited the most potent inhibitory effect against S. aureus and A. baumannii growth with MIC 25 mg/ml. In the case of, in vivo study and regarding S. aureus, it was found a significant difference (P < 0.05) using methanol crude extract but in other words, there is no effect of this extract on A. baumannii. Conclusion: Methanol crude extract of P. ostreatus was the powerful one as an antibacterial agent against the tested S. aureus and A. baumannii than the other extracts which are chloroform and aqueous in vitro and in vivo models except the later design was not useful in A. baumannii.

  • Abstract Audio

    No Audio file found

  • About the authors and Affiliations

    Author(s) Name:

    Asmaa Hussein Gutef 1 *, Azhar A. F. Al-Attraqchi 2 , Amer Taleb Tawfeeq3 , Haider Bahaa Saheb4

    Affiliation(s) Name:

    1 Department of Basic Science, College of Dentistry, Wasit University, Kut, Iraq,

    2 Department of Microbiology, College of Medicine, Al-Nahrain University, Baghdad, Iraq,

    3 Iraqicenters for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq,

    4 Dean of the College of Pharmacy/Al-Nahrain University, Baghdad, Iraq

    *Corresponding author: Asmaa Hussein Gutef, Department of Basic Science, College of Dentistry, Wasit University, Kut, Iraq.

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    0 View More
  • How to Cite my Article.

    Author:

    Asmaa Hussein Gutef 1 *, Azhar A. F. Al-Attraqchi 2 , Amer Taleb Tawfeeq3 , Haider Bahaa Saheb4

    Title:Evaluation of antibacterial potential of fruiting body extracts of Pleurotus ostreatus in vitro and in vivo study
    Journal:Drug Invention Today
    Vol(issue):14 (June )
    Year:2020
    Page No: (854-863)
  • Experimental Methods Keywords

    Methodology:antibacterial potential
    Research Materials:Edible mushroom

Keywords

Edible mushroom Methanol crude extract Multidrug resistance Murine model

Our Services

Most Downloaded List