Journal: Journal of Pharmacy Research

Article Id: JPRS-QA-0000523
Title: Validation of reverse phase high performance liquid chromatography method of metolazone and its determination in bulk drug and pharmaceutical dosage form
Category: Quality Assurance
Section: Research Article
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  • Abstract

    To develop and validate a sensitive, selective, precise high performance liquid chromatographic (HPLC) method of analysis for metolazone both as bulk drug and formulations. A new sensitive and reproducible HPLC method was developed and validated for the determination of metolazone in tablets. The separation was achieved by using a JASCO HPLC system 2000 series comprising of JASCO PU 2080 plus pump, JASCO UV 2075 plus detector, Rheodyne manual injector fitted with 100 μl sample loop. The analytical column used was thermo C18 (250 X 4.6mm; 5μm) at ambient temperature using a mobile phase consisting of Acetonitrile: Water (50:50). The method was validated for accuracy, precision, linearity, reproducibility and robustness and statistical comparison was performed by analysis of variance. The developed HPLC technique is precise, specific, accurate and stable. Statistical analysis proves that the method is reproducible, selective and suitable to be applied for analysis of metolazone in commercial pharmaceutical dosage form for routine quality control application.

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    Author(s) Name:

    Varsha Jadhav*, Prashant Mande and Vilasrao Kadam

    Affiliation(s) Name:

    Department of Quality Assurance, Bharati Vidyapeeth’s College of Pharmacy,
    Navi-Mumbai – 400614, MH, India


    Received on: 12-09-2008; Accepted on: 04-01-2009

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    Author:

    Varsha Jadhav*, Prashant Mande and Vilasrao Kadam

    Title:Validation of reverse phase high performance liquid chromatography method of metolazone and its determination in bulk drug and pharmaceutical dosage form
    Journal:Journal of Pharmacy Research
    Vol(issue):2 (May)
    Year:2009
    Page No: (961-963)
  • Experimental Methods Keywords

    Methodology:RP-HPLC, Dosage form
    Research Materials: Metolazone

Keywords

Metolazone RP-HPLC Dosage form

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