Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-0000583
Title: Effect of alkaloidal fraction of Elaeocarpus ganitrus Roxb. seeds on murine in vitro immune parameters
Category: Pharmaceutics
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    The alkaloidal fraction of Elaeocarpus ganitrus seeds (EGAF) was prepared and evaluated for in vitro effects on murine cells for release of immune mediators and cell proliferation. The EGAF was tested at concentrations ranging between 6.5-832μg/ml. The release of mediators assayed from peritoneal exudates cells (PEC) include nitric oxide (NO), superoxide (NBT reduction), lysosomal and myeloperoxidase. The fraction was also evaluated for cell proliferation in sulforhodamine B (SRB) assay on murine PEC, splenocytes and bone marrow cells. The data was statistically compared by using one way ANOVA followed by Dunnet’s multiple comparisons test. The fraction showed significant stimulation (P<0.05) of NBT reduction, NO release and myeloperoxidase activity of PEC cells. The effect on lysosomal enzyme activity was not significant. Significant proliferation (P<0.05) was observed in SRB assay with PEC and bone marrow cells. The effect of EGAF was not significant on proliferation of splenocytes. Positive control, phytohaemagglutinin showed stimulation of release of all the tested mediators and proliferation of immune cells. In conclusion, the EGAF showed in vitro stimulation of immune mediators from PEC and proliferation of immune cells.

  • Abstract Audio

    Click Here

  • About the authors and Affiliations

    Author(s) Name:

    Amolkumar Kisanrao Hule*, Archana Ramesh Juvekar

    Affiliation(s) Name:

    *Department of Pharmaceutical Sciences and Technology, University Institute of Chemical Technology (UICT),Nathalal Parikh Marg, Matunga (E), Mumbai-400019, (M.S.), India


    Received on: 09-04-2009; Accepted on:24-06-2009

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    2 View More
  • How to Cite my Article.

    Author:

    Amolkumar Kisanrao Hule*, Archana Ramesh Juvekar

    Title:Effect of alkaloidal fraction of Elaeocarpus ganitrus Roxb. seeds on murine in vitro immune parameters
    Journal:Journal of Pharmacy Research
    Vol(issue):2 (August)
    Year:2009
    Page No: (1261-1265)
  • Experimental Methods Keywords

    Methodology:murine in vitro immune parameters
    Research Materials:Elaeocarpus ganitrus Roxb.

Keywords

Nitro blue tetrazolium; macrophages; myeloperoxidase; sulforhodamine B

Our Services

Most Downloaded List