Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-0000622
Title: Development and validation of new RP-HPLC method with UV detection for the determination of rifampicin in plasma
Category: Pharmaceutics
Section: Research Article
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    To standardize a high-performance liquid chromatography (HPLC) method for the determination of rifampicin in plasma by external standard method.Material and Methods: A simple, specific and sensitive HPLC method was developed for the determination of rifampicin in plasma. Separation was achieved by reverse phase chromatography on a C18 column with a mobile phase composition of phosphate buffer pH 7.4: methanol (75:25 v/v) at 475 nm.Results: The retention time of rifampicin was 2.54 minutes. The assay was linear from 0.05 to 20.0 mgm /mL for plasma. Both intra-day and inter-day accuracy and precision data showed good reproducibility. Recoveries (extraction efficiency) for drug were greater than 90% in plasma .The plasma method was precise (total coefficient of variation ranged from 1.46 to 6.71%) for the analytes.Conclusion: The HPLC method described is sensitive, selective and linear for the wide range of concentrations for rifampicin in plasma. Thus, the method developed is well suited for the pharmacokinetic studies.

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    Author(s) Name:

    P.Sabitha1*, J. Vijaya Ratna2 and K. Ravindra Reddy1

    Affiliation(s) Name:

    1*Department of Pharmaceutics; P. Rami Reddy Memorial College of Pharmacy, Kadapa, Andhra Pradesh,PIN:516003 India. 2University College of Pharmaceutical sciences, Andhra University, Visakhapatnam,India.

    Received on: 20-05-2009; Accepted on:15-07-2009

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    Author:

    P.Sabitha1*, J. Vijaya Ratna2 and K. Ravindra Reddy1

    Title:Development and validation of new RP-HPLC method with UV detection for the determination of rifampicin in plasma
    Journal:Journal of Pharmacy Research
    Vol(issue):2 (October)
    Year:2009
    Page No: (1561-1564)
  • Experimental Methods Keywords

    Methodology:RP-HPLC method
    Research Materials: Rifampicin

Keywords

Rifampicin HPLC Reversed phase chromatography

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