Journal: Journal of Pharmacy Research

Article Id: JPRS-PA-0000626
Title: A sensitive and selective RP-HPLC method for the determination of lamivudine and stavudine in tablets
Category: Pharmaceutical Analysis
Section: Research Article
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  • Abstract

    A simple, fast, precise and accurate reverse phase high performance liquid chromatographic method developed for the simultaneous determination and validation of lamivudine (3TC) and stavudine (d4T)  in tablets. A sunfire C18, 250 × 4.6 mm, 5 mm particle size column in isocratic mode was used with mobile phase comprising of methanol: 0.1 % w/v of ammonium acetate, adjusted to pH 3.8 with glacial acetic acid in the ratio of 15:85, v/v. The flow rate was set at 1.2 ml per minute with UV detection at 266 nm. The retention time of 3TC, d4T was found 5.6 and 8.9 minute respectively. Linearity for lamivudine and stavudine were found in the range of 75-225mg/ml and 20-60mg/ml respectively. Percentage recoveries were obtained in the range of 98.97 % to 99.71 % for lamivudine and 99.22 % to 99.59 % for stavudine. The proposed method is precise, accurate, selective, reproducible, and rapid for the simultaneous estimation of lamivudine and stavudine in tablet dosage forms.

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  • About the authors and Affiliations

    Author(s) Name:

    Pawan K Saini1, Raman M Singh1*, Chhoten L Jain2, Satish C Mathur1 and  Gyanendra N Singh1

    Affiliation(s) Name:

    1Indian Pharmacopoeia Commission, Ministry of H. and F.W., Govt. of India, Sector-23, Rajnagar, Ghaziabad (U.P.)- 201 002, India.
    2Department of Chemistry, M.M.H. College, Ghaziabad (U.P.)- 201 001, India.


    Received on: 20-05-2009; Accepted on:15-07-2009

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    Author:

    Pawan K Saini1, Raman M Singh1*, Chhoten L Jain2, Satish C Mathur1 and  Gyanendra N Singh1

    Title:A sensitive and selective RP-HPLC method for the determination of lamivudine and stavudine in tablets
    Journal:Journal of Pharmacy Research
    Vol(issue):2 (October)
    Year:2009
    Page No: (1598-1600)
  • Experimental Methods Keywords

    Methodology: RP-HPLC Method and Simultaneous Estimation.
    Research Materials:Lamivudine, Stavudine

Keywords

Lamivudine Stavudine RP-HPLC Method and Simultaneous Estimation.

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