Journal: Journal of Pharmacy Research
Title: HPTLC Method Development and Validation for the Simultaneous Estimation of Amlodipine Besylate and Nebivolol Hydrochloride in tablet dosage form
Category: Pharmaceutical Analysis
Section: Research Article
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Abstract
The simple, accurate and precise method for Simultaneous quantification of Nebivolol hydrochloride ( NEB-H) and Amlodipine Besylate (AML) in tablets by HPTLC methods were developed In this method, the chromatograms were developed using a mobile phase of Methylene Chloride : Methanol : Ammonia ( 8.5:1:0.5 v/v ) on precoated plate of silica gel 60 F254 and quantified by densitometric absorbance mode at 285 nm. The Rf of AML and NEB-H were 0.19 and 0.41 respectively. Recovery studies of 99.96 – 102.11%, percentile relative standard deviation of not more than 0.8 and correlation coefficient (linearity range) of 0.9984 – 0.9998 shows that developed methods were accurate and precise for AML and NEB-H respectively. The LOD and LOQ values were found to be 30ng/ml, 30ng/ml and 80ng/ml, 80ng/ml for AML and NEB-H respectively. The mean percentage recovery values close to 100% it indicates there is no interferences of additives with Nebivolol hydrochloride (NEB-H) and Amlodipine Besylate (AML) present in tablet dosage forms. The method has been validated as per ICH guide lines. This method can be employed for the routine analysis of tablets containing AML and NEB-H.
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About the authors and Affiliations
Author(s) Name: B.Dhandapani*1, N.Anjaneyulu1, Y.Venkateshwarlu2 and Shaik Harun Rasheed2
Affiliation(s) Name: *1K.M.C.H. College of Pharmacy, Kovai Estate, Kalappatti Road, Coimbatore – 641035, Tamilnadu.
2Department of Pharmaceutical Analysis, Donbosco College of pharmacy, Pulladi gunta, Etukuru,Guntur, Andhra Pradesh.*Corresponding author.
B.Dhandapani,
3/17, Rajagobalapuram Street,
Thirumanamedu Post,Lalgudi Taluk,
Tiruchirappalli District – 621218. Tamilnadu,India
Received on: 20-09-2009; Revised on: 16-10-2009; Accepted on:15-12-2009-
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Author: B.Dhandapani*1, N.Anjaneyulu1, Y.Venkateshwarlu2 and Shaik Harun Rasheed2
Title: HPTLC Method Development and Validation for the Simultaneous Estimation of Amlodipine Besylate and Nebivolol Hydrochloride in tablet dosage form Journal: Journal of Pharmacy Research Vol(issue): 3 (February) Year: 2010 Page No: (332-334) -
Experimental Methods Keywords
Methodology: High Performance Thin Layer Chromatography (HPTLC) Research Materials: Nebivolol hydrochloride ( NEB-H), Amlodipine Besylate (AML)
Keywords
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