Journal: Journal of Pharmacy Research

Article Id: JPRS-PA-0000677
Title: HPTLC Method Development and Validation for the Simultaneous Estimation of Amlodipine Besylate and Nebivolol Hydrochloride in tablet dosage form
Category: Pharmaceutical Analysis
Section: Research Article
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    The simple, accurate and precise method for Simultaneous quantification of Nebivolol hydrochloride ( NEB-H) and Amlodipine Besylate (AML) in tablets by HPTLC methods were developed In this method, the chromatograms were developed using a mobile phase of Methylene Chloride : Methanol : Ammonia ( 8.5:1:0.5 v/v ) on precoated plate of silica gel 60 F254 and quantified by densitometric absorbance mode at 285 nm. The Rf of AML and NEB-H were 0.19 and 0.41 respectively. Recovery studies of 99.96 – 102.11%, percentile relative standard deviation of not more than 0.8 and correlation coefficient (linearity range) of 0.9984 – 0.9998 shows that developed methods were accurate and precise for AML and NEB-H respectively. The LOD and LOQ values were found to be 30ng/ml, 30ng/ml and 80ng/ml, 80ng/ml for AML and NEB-H respectively. The mean percentage recovery values close to 100% it indicates there is no interferences of additives with Nebivolol hydrochloride (NEB-H) and Amlodipine Besylate (AML) present in tablet dosage forms. The method has been validated as per ICH guide lines. This method can be employed for the routine analysis of tablets containing AML and NEB-H.

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    Author(s) Name:

    B.Dhandapani*1, N.Anjaneyulu1, Y.Venkateshwarlu2 and Shaik Harun Rasheed2

    Affiliation(s) Name:

    *1K.M.C.H. College of Pharmacy, Kovai Estate, Kalappatti Road, Coimbatore – 641035, Tamilnadu.
    2Department of Pharmaceutical Analysis, Donbosco College of pharmacy, Pulladi gunta, Etukuru,Guntur, Andhra Pradesh.

    *Corresponding author.
    B.Dhandapani,
    3/17, Rajagobalapuram Street,
    Thirumanamedu Post,Lalgudi Taluk,
    Tiruchirappalli District – 621218. Tamilnadu,India


    Received on: 20-09-2009; Revised on: 16-10-2009; Accepted on:15-12-2009

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    Author:

    B.Dhandapani*1, N.Anjaneyulu1, Y.Venkateshwarlu2 and Shaik Harun Rasheed2

    Title:HPTLC Method Development and Validation for the Simultaneous Estimation of Amlodipine Besylate and Nebivolol Hydrochloride in tablet dosage form
    Journal:Journal of Pharmacy Research
    Vol(issue):3 (February)
    Year:2010
    Page No: (332-334)
  • Experimental Methods Keywords

    Methodology:High Performance Thin Layer Chromatography (HPTLC)
    Research Materials: Nebivolol hydrochloride ( NEB-H), Amlodipine Besylate (AML)

Keywords

Nebivolol hydrochloride ( NEB-H) Amlodipine Besylate (AML) High Performance Thin Layer Chromatography (HPTLC)

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