Journal: Journal of Pharmacy Research
Title: Antimicrobial activity and phytochemical screening of five wild plants against Escherichia coli, Bacillus subtilis and Staphylococcus aureus
Category: Microbiology
Section: Research Article
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Abstract
The antimicrobial activity of plant oils and extracts has been recognized for many years. In the present study, the antibacterial activity of methanol and hexane leaf extracts of Datura stramonium, Ricinus communis, Calotropis gigantean, Malva verticillata and Malvastrum coromandelium was evaluated against Escherichia coli, Staphylococcus aureus and Bacillus subtilis by agar well diffusion method. The maximum antimicrobial activity expressed in terms of zone of inhibition was shown by methanol and hexane leaf extracts of Datura stramonium (29 to 35mm) followed by Ricinus communis (15 to 24mm), Calotropis gigantean (12 to 25mm) and Malva verticillata (12 to 16mm) against all the test organisms. The present study also revealed that methanolic leaf extracts exhibited more effective antibacterial activity as compared to hexane extracts. Phytochemical screening of leaf extracts of five wild plants revealed the presence of steroids, flavonoids and cardiac glycosides as active components in both methanol and hexane extracts. Tannins were found to be absent in both the extracts. This study scientifically validates the use of plants as a potent antibacterial agents.
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About the authors and Affiliations
Author(s) Name: Pranay Jain1*, Dinesh Bansal2 ,Pragya Bhasin2 ,Anjali 3
Affiliation(s) Name: 1Department of Biotechnology, University Institute of Engineering and Technology,Kurukshetra University, Kurukshetra , India
2Department of Environmental Engineering, IIT, Mumbai, India*Corresponding author.
Dr. Pranay Jain
Asstt. Professor, Biotechnology
UIET, Kurukshetra University, Kurukshetra, India
Received on: 16-02-2010; Revised on: 14-03-2010; Accepted on:15-04-2010-
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Author: Pranay Jain1*, Dinesh Bansal2 ,Pragya Bhasin2 ,Anjali 3
Title: Antimicrobial activity and phytochemical screening of five wild plants against Escherichia coli, Bacillus subtilis and Staphylococcus aureus Journal: Journal of Pharmacy Research Vol(issue): 3 (June) Year: 2010 Page No: (1260-1262) -
Experimental Methods Keywords
Methodology: Antibacterial activity, agar well diffusion method Research Materials: Datura stramonium, Ricinus communis, Calotropis gigantean, Malva verticillata and Malvastrum coromandelium
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