Journal: Journal of Pharmacy Research

Article Id: JPRS-PHDRS-00008
Title: In vitro Antioxidant Activity from Leaves of Oroxylum indicum (L.) Vent. -A North Indian Highly Threatened and Vulnerable Medicinal Plant
Category: Pharmacognosy and Herbal Drugs related study
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    Free radical scavenging potential of the different extracts of leaves of Oroxylum indicum (L.) Vent. (Bignoniaceae), one of the widely used medicinal plant, was evaluated in vitro by using diphenyl-picryl-hydrazyl (DPPH) assay. In this method the antioxidants present in the plant extracts reacted with DPPH, which is a stable  free radical and converted it to 1,1-diphenyl-1,2-picryl, hydrazine. The scavenging effect of plant extracts and standard (L-ascorbic acid) on the DPPH radical decreases in the following order: L-ascorbic acid > Ethyl acetate (I) >Methanol (II) > Water (III) and it was found to be 97.4%, 61.4%, 40.8% and 29.2% at concentration of 100 mg/mL, respectively. The results were expressed as IC50. Ascorbic acid which was used as a standard showed an IC50 of 24.0 mg/mL, whereas, the crude ethyl acetate (I), methanolic (II) and water (III) extracts of leaves of O. indicum showed antioxidant activity with IC50 values of 49.0, 55.0 and 42.5 respectively at 100 mg/mL concentration. None of the extracts were found to be more active than the standard i.e. ascorbic acid. But still, free radical scavenging activity of water (III) and ethyl acetate (I) extracts of leaves of O. indicum (L.) was confirmed in the present investigation. In the present study, different extracts of O. indicum leaves showed concentration dependent free radical scavenging  activity. Significant results were recorded first time in the given estimated parameters from different extracts of leaves of O. indicum, a North Indian highly threatened and vulnerable medicinal plant.

    Cite this article as: In vitro Antioxidant Activity from Leaves of Oroxylum indicum (L.) Vent. -A North Indian Highly Threatened and Vulnerable Medicinal Plant,Raghbir C. Gupta1 *, Vivek Sharma1 , Nisha Sharma1 , Neeraj Kumar2 , Bikram Singh2, Journal of Pharmacy Research, Vol.1.Issue 1. July-September 2008,65-72.

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    Author(s) Name:

    Raghbir C. Gupta1 *, Vivek Sharma1, Nisha Sharma1, Neeraj Kumar2, Bikram Singh2

    Affiliation(s) Name:

    1Botany Department, Punjabi University, Patiala-147002 (Punjab) India.
    2N.P.P. Division, Institute of Himalayan Bioresource Technology, CSIR, Palampur-176061
    (Himachal Pradesh) India.

     

    For correspondence:

    Raghbir C. Gupta Professor & Head,

    Department of Botany, Punjabi University,

    Patiala- 147002 (Punjab) India.


    Email: rcgupta53@gmail.com


    Received on: 08-06-2008; Accepted on :16-07-2008

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    Author:

    Raghbir C. Gupta1 *, Vivek Sharma1, Nisha Sharma1, Neeraj Kumar2, Bikram Singh2

    Title:In vitro Antioxidant Activity from Leaves of Oroxylum indicum (L.) Vent. -A North Indian Highly Threatened and Vulnerable Medicinal Plant
    Journal:Journal of Pharmacy Research
    Vol(issue):1(1)
    Year:2008
    Page No: (65-72)
  • Experimental Methods Keywords

    Methodology:diphenyl-picryl-hydrazyl (DPPH) assay
    Research Materials:Oroxylum indicum

Keywords

Oroxylum indicum antioxidant activity extracts DPPH assay.

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