Journal: Journal of Pharmacy Research

Article Id: JPRS-P'Col-000085
Title: Protective effect of catechin from black tea in an in-vitro model of myocardial ischemic reperfusion injury
Category: Pharmacology
Section: Research Article
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    Aim: Present study was designed to compare cardio-protective activity of crude aqueous extract and catechin fractions of black tea on the oxidative stress model of myocardial ischemic reperfusion injury in rat. Method: Black tea extract and catechin fraction were administered to male Wister rats weighing between 200-250 g in three different doses (n=6), by gastric gavage at a dose of 500 mg/kg body weight (BT1), 1000 mg/kg body weight (BT2), 1500 mg/kg body weight (BT3) and catechin fraction at a dose of 10 mg/kg body weight (CT1), 20 mg/kg body weight (CT2), 30 mg/kg body weight (CT3) daily for thirty days. Rat’s heart was subjected to in vitro global ischemic reperfusion injury (5 min perfusion, 9 min no flow and 12 min reperfusion). Biochemical parameters like TBARS, SOD, GSH and catalase were measured in treated groups at basal level and in in vitro ischemic reperfusion injury of the treated groups. Results: Pre-treatment with the black tea crude extract on the dose of both 500 & 1000 mg/kg and catechin 10, 20 & 30 mg/kg has a definite, dose-dependent modulatory effect on the antioxidant milieu of heart. CT2IR treated group offered better cardio-protection when subjected to IR injury. No definite pattern of change in the level of TBARS and endogenous antioxidants was observed. Hence these modulatory actions seem to be enzyme-specific. Conclusion: From these findings it has been concluded that Catechin fraction in a smaller dose (20 mg/kg) shows significant cardioprotective activity when compared with other doses and parent black tea extract. Further research is required to find out the mechanism of action of Catechin for cardioprotection.

    DOI: https://dx.doi.org/10.6084/m9.figshare.3168115.v1

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    Author(s) Name:

    Verma R1,2, Das M1, Mohamed Saleem T.S 1,4, Kumar L1,5 , Gauthaman K1,3*

    Affiliation(s) Name:

    1Himalayan Pharmacy Institute, Majhitar, East Sikkim, India.
    2Department of Pharmaceutical Chemistry, 5Department of Pharmaceutics,
    Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
    3Department of pharmacology, College of pharmacy, King Khalid University, Abha Kingdom of Saudi Arabia.
    4Department of Pharmacology, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet-516126, A.P., India.

    *Corresponding author.
    Karunakaran Gauthaman
    Associate Professor,
    Department of Pharmacology,
    College of Pharmacy,
    King Khalid University,
    Abha Kingdom of Saudi Arabia.
    Email id: gauthamank@gmail.com

    Received on:21-11-2013; Revised on: 08-12-2013; Accepted on:11-12-2013

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    Author:

    Verma R1,2, Das M1, Mohamed Saleem T.S 1,4, Kumar L1,5 , Gauthaman K1,3*

    Title:Protective effect of catechin from black tea in an in-vitro model of myocardial ischemic reperfusion injury
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (January )
    Year:2014
    Page No: (1-6)
  • Experimental Methods Keywords

    Methodology:Preliminary phytochemical screening,Production of in-vitro myocardial ischemic-reperfusion injury,Biochemical parameters
    Research Materials:Catechin, Tea Polyphenol

Keywords

Antioxidants Ischemic reperfusion injury Catechin Tea Polyphenol

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