Journal: Drug Invention Today

Article Id: JPRS-DFP-0000853
Title: Vitex agnus castus Molecular Marker Compounds Extraction and Optimization Using HPLC with ELS Detector
Category: Drugs from Plants
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Vitex agnus castus extracts in different solvent ratios were analyzed using High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD). The markers of our interest in Vitexagnus castus are Agnuside, Casticin and Vitexyl acetone. To correlate the retention time of the molecular marker compounds with Vitex agnus castus extract were compared with standard molecular markers of Agnuside, Casticin and Vitexyl acetone using same conditions of that of extract and were analyzed statistically. Apart from the results ELS Detector is regarded as a valuable alternative to UV detection system for liquid chromatographic analysis of substances that does not contain a strong chromophore. As the markers do not contain strong chromophore, it is difficult to identify it UV detection system in High performance liquid chromatography. So an alternative is the Evaporative Light scattering Detector which is an effective tool for the identification of the compounds which do not contain chromophores. Present study concludes that ELS Detection system is more efficient than the UV/Vis detection system.

  • Abstract Audio

    Click Here

  • About the authors and Affiliations

    Author(s) Name:

    Somasekhar Penumajji1, Varaprasad Bobbarala2*, andK. Chandrasekhar Naidu 3

    Affiliation(s) Name:

    1Vivimed labs Limited, 2nd, 4th Floor, Veeranag towers, Hyderabad
    2For U Biosciences, A/4A, Park lane Residency, East point colony, Visakhapatnam-17
    3Department of Botany, Andhra University, Visakhapatnam-3

    *Corresponding author.
    Dr. Varaprasad Bobbarala
    Scientist In-Charge,
    For U Biosciences/IMMA Labs,
    A/4A, Park lane Residency, East point colony,
    Visakhapatnam, A.P-530017, India.


    Received on: 20-08-2009; Revised on: 21-09- 2009; Accepted on:15-10-2009

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    2 View More
  • How to Cite my Article.

    Author:

    Somasekhar Penumajji1, Varaprasad Bobbarala2*, andK. Chandrasekhar Naidu 3

    Title:Vitex agnus castus Molecular Marker Compounds Extraction and Optimization Using HPLC with ELS Detector
    Journal:Drug Invention Today
    Vol(issue):1 (January 2009)
    Year:2009
    Page No: (39-45)
  • Experimental Methods Keywords

    Methodology:Evaporative light scattering detector, High Performance Liquid chromatography.
    Research Materials:Vitexagnus castus

Keywords

Vitexagnus castus Evaporative light scattering detector High Performance Liquid chromatography.

Our Services

Most Downloaded List