Journal: Drug Invention Today

Article Id: JPRS-PS-0000862
Title: In vitro and in vivo evaluation of immunomodulatory activity of methanol extract of Momordica charantia fruits
Category: Pharmacological Screening
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    The fruits of Momordica charantia have been traditionally used in India and other Asian countries. The methanol extract of Momordica charantia fruits (MCM) was evaluated for immunomodulatory activity using in vitro and in vivo methodologies. Effect of extract was evaluated at various concentrations (832 – 6.5 μg/ml) for secretion of mediators like nitric oxide, superoxide, lysosomal enzyme and myeloperoxidase activity of isolated murine peritoneal macrophages. The extract showed stimulation of nitric oxide, lysosomal enzyme and myeloperoxidase activity. The extract was also evaluated for in vivo phagocytic activity by carbon clearance assay in mice and it showed significant increase in the phagocytic index at 100, 200 and 400mg/kg dose. The effect of the extract on delayed type hypersensitivity (DTH) and antibody titre assay were evaluated in ovalbumin immunized mice. MCM showed no significant effect on DTH response but significant stimulation of antibody titer at 200 and 400mg/kg dose. The effect of the extract in cyclophosphamide induced myelosuppressed mice was not significant. The results suggest that the extract stimulated non-specific murine immune system, both in vitro and in vivo.

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    Author(s) Name:

    Archana Ramesh Juvekar*, Amolkumar Kisanrao Hule, Sachin Sridhar Sakat, Vivek Avinash Chaughule

    Affiliation(s) Name:

    Department of Pharmaceutical Sciences and Technology, University Institute of Chemical Technology (UICT), Mumbai-400019, (M.S.), India

    *Corresponding author.
    Prof. (Mrs.) Archana R. Juvekar,
    Department of Pharmaceutical Sciences and Technology, University Institute
    of Chemical Technology (UICT), Mumbai-400019, (M.S.), India


    Received on: 22-06-2009; Revised on: 21-07- 2009; Accepted on:15-09-2009

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    Author:

    Archana Ramesh Juvekar*, Amolkumar Kisanrao Hule, Sachin Sridhar Sakat, Vivek Avinash Chaughule

    Title:In vitro and in vivo evaluation of immunomodulatory activity of methanol extract of Momordica charantia fruits
    Journal:Drug Invention Today
    Vol(issue):1(2)
    Year:2009
    Page No: (89-94)
  • Experimental Methods Keywords

    Methodology:In vitro and in vivo evaluation of immunomodulatory activi
    Research Materials:Momordica charantia fruits

Keywords

Macrophages; nitric oxide; phagocytosis; ovalbumin; antibody

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