Journal: Drug Invention Today

Article Id: JPRS-MSNS-0000933
Title: Antimicrobial activity of Allium sativum ethanolic extract against food associated bacteria and fungi
Category: Microbiological Study (on Natural/ Synthetic drug)
Section: Research Article
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    The present study was designed to evaluate the in vitro antimicrobial activity of ethanolic extract of Allium sativum (garlic) against Gram-positive and Gram-negative food associated bacteria (Bacillus subtilis, B. megaterium, B. polymyxa, B. sphaericus, Staphylococcus aureus and Escherichia coli) and molds (Penicillium oxalicum, Aspergillus flavus, A. luchuensis, Rhizopus stolonifer, Scopulariopsis sp. and Mucor sp.) assayed by agar well diffusion method and poisoned food technique, respectively. The growth inhibition zone in garlic ethanolic extract against bacteria was in the range of 20mm to 31mm and in molds the percent mycelial growth inhibition  ranged between 20% and 50%. The garlic extract was not effective against P. oxalicum. The MIC values of garlic ethanolic extract for different bacterial and fungal isolates ranged from 10 mg/ml to 20mg/ml. The value of MBC and MFC equaled the MIC. Based on this finding, it may be suggested that these extracts may be used as natural antimicrobial additives to reclaim the shelf-life of foods.

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  • About the authors and Affiliations

    Author(s) Name:

    Ram Kumar Pundir1 and Pranay Jain2

    Affiliation(s) Name:

    1Lecturer in Biotechnology,Kurukshetra Institute of Technology and Management,Bhor Siadan, Kurukshetra-136119,Haryana, India
    2Lecturer in Biotechnology,University Institute of Engineering and Technology,Kurukshetra University, Kurukshetra-136119,Haryana, India

    *Corresponding author.
    Ram Kumar Pundir
    Lecturer in Biotechnology, Kurukshetra
    Institute of Technology and Management,
    Bhor Saidan, Kurukshetra-136119, Haryana, India

    Received on: 20-11-2009; Revised on: 21-12- 2009; Accepted on:11-02-2010

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    Ram Kumar Pundir1 and Pranay Jain2

    Title:Antimicrobial activity of Allium sativum ethanolic extract against food associated bacteria and fungi
    Journal:Drug Invention Today
    Vol(issue):2 (April)
    Page No: (229-232)
  • Experimental Methods Keywords

    Methodology:Antimicrobial activity,Zone of inhibition,MIC
    Research Materials:Allium sativum


Antimicrobial activity food associated microorganisms Zone of inhibition garlic MIC

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