Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-000097
Title: Development and validation of RP-HPLC method for quantitative analysis of Miglitol in pure and pharmaceutical formulations
Category: Pharmaceutics
Section: Research Article
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    A reverse phase high performance liquid chromatographic method was performed by using Zodiac C18column (250mmX4.6mmX5μ particle size) with UV detection at 216 nm. An isocratic mobile phase consisting of Acetonitrile:Methanol:Potassium dihydrogen Phosphate50:35:15(v/v/v) at a flow rate of 1ml/min.The retention time for Miglitol was 3.84 min. The method was linear in the concentration range of 40-100 μg/ml of Miglitol with the correlation coefficient of 0.999. The method was validated for linearity,accuracy, precision, limit of detection,limit of quantification,robustness and ruggedness.Recovey of Miglitol was found to be 99% to 102%.The developed reverse phase high performance liquid chromatographic method was simple, sensitive, precise and accurate and the method was found suitable for estimating in tablet dosage form.

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    Author(s) Name:

    D.Madhu latha * 1, K. Ammani 2 ,Y. Indira Muzib 3,P. Jitendra kumar 4,V. Sai Kishore5

    Affiliation(s) Name:

    1. Department of Biotechnology, Acharya Nagarjuna university, Nagarjuna nagar 522510,Andhra Pradesh, India
    2. Department of Botany and Microbiology , Acharya Nagarjuna university, Nagarjuna nagar 522510,Andhra pradesh,India
    3. Department of Pharmaceutics, Institute of Pharmaceutical Technology,Sri Padmavati Mahila university, Tirupati 517501, Andhra Pradesh, India
    4.School of Pharmaceutical Education and Research,Berhampur university,Berhampur 760002,Odisha,India
    5. Bapatla college of Pharmacy,Bapatla 522101 ,Guntur,Andhra Pradesh,India

    *Corresponding author.
    D.Madhu latha,
    Department of Biotechnology,
    Acharya Nagarjuna university,
    Nagarjuna nagar- 522510,
    Andhra Pradesh, India
    Email addresses:pjksai@rediffmail.com

    Received on:22-11-2013; Revised on: 10-12-2013; Accepted on:26-12-2013

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    Author:

    D.Madhu latha * 1, K. Ammani 2 ,Y. Indira Muzib 3,P. Jitendra kumar 4,V. Sai Kishore5

    Title:Development and validation of RP-HPLC method for quantitative analysis of Miglitol in pure and pharmaceutical formulations
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (January )
    Year:2014
    Page No: (64-67)
  • Experimental Methods Keywords

    Methodology:RP-HPLC method
    Research Materials:Miglitol

Keywords

Miglitol C18 column Reverse phase Validation

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