Journal: Journal of Pharmacy Research

Article Id: JPRS-PA-000099
Title: Development and validation of RP-HPLC method for quantitative analysis of Modafinil in pure and pharmaceutical formulations
Category: Pharmaceutical Analysis
Section: Research Article
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    The present work aim to develop a simple, accurate and sensitive RP-HPLC method and validated for the estimation of Modafinil in pure drug and it’s tablet dosage form.A reverse phase high performance liquid chromatographic method was performed by using Agilent,XDBC18 column (100 mmX 4.6 mmX5ì paricle size) with UV detection at 225 nm. An isocratic mobile phase consisting of Potassium dihydrogen Phosphate Acetonitrile:50:50 (v/v) at a flow rate of 1ml/min. The retention time for Modafinil was 4.32 min. The method was linear in the concentration range of 12.5-75 μg/ml of Modafinil with the correlation coefficient of 0.999. The method was validated for linearity,accuracy,precision,limit of detection,limit of quantification,robustness and ruggedness.Recovey of Modafinil was found to be 99% to 101%.The developed reverse phase high performance liquid chromatographic method was simple, sensitive, precise and accurate and the method was found suitable for estimating in tablet dosage form.

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    Author(s) Name:

    Y. Indira Muzib1,P. Jitendra kumar2*, Desireddy R. B.3, N. Ventateswaralu4,V. Sai Kishore 5

    Affiliation(s) Name:

    1.Institute of Pharmaceutical Technology,Sri Padmavati Mahila Visvavidyalayam, Tirupati 517501, Andhra Pradesh, India.
    2.School of Pharmaceutical Education and Research,Berhampur university,Berhampur 760002 ,Odisha, India.
    3,4.Nalanda Instituteof Pharmaceutical Sciences,Kantepudi,Satteanpalli 522403,Guntur, Andhra Pradesh,India.
    5. Bapatla college of Pharmacy,Bapatla 522101 ,Guntur,Andhra Pradesh, India.

    *Corresponding author.
    P.Jitendrakumar,
    School of Pharmaceutical Education and Research,
    Berhampur university,
    Berhampur 760002,Odisha,India
    Email addresses:pjksai@rediffmail.com


    Received on:12-12-2013; Revised on: 27-12-2013; Accepted on: 07-01-2014

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    Author:

    Y. Indira Muzib1,P. Jitendra kumar2*, Desireddy R. B.3, N. Ventateswaralu4,V. Sai Kishore 5

    Title:Development and validation of RP-HPLC method for quantitative analysis of Modafinil in pure and pharmaceutical formulations
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (January )
    Year:2014
    Page No: (73-76)
  • Experimental Methods Keywords

    Methodology:RP-HPLC method
    Research Materials:Modafinil

Keywords

Modafinil C18 column Reverse phase Validation

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