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Journal of Pharmacy Research
ISSN NO: 0974-6943
The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified when, we/or author get comments from any readers. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry,  Nursing, Paramedical, prescription etc  fields).
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Journal Metrics for this Journal of Pharmacy Research (Source ID: 21100325431): 2015(SNIP) Source Normalized Impact Per Paper : 0.575; SCImago Journal Rank (SJR):0.787; Impact Per Publication : 0.789 CiteScore 2016: 0.93(Top level : Life Science)

Year SJR Cites per document Year Value
2014 0.607 Cites / Doc. (4 years) 2014 0.607
2015 0.787 Cites / Doc. (4 years) 2015 0.789
2016 0.926 Cites / Doc. (4 years) 2016 0.926
    Cites / Doc. (3 years) 2014 0.607
    Cites / Doc. (3 years) 2015 0.789
    Cites / Doc. (2 years) 2014 0.607
    Cites / Doc. (2 years) 2015 0.789
2016   Cites / Doc. (4 years) 2016 0.926
Cites Year Value
External Cites per document 2014 0.607
External Cites per document 2015 0.789
External Cites per document 2016 0.926
Cites per document 2014 0.607
Cites per document 2015 0.789
Cites per document 2016 0.926

Manuscripts Published

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PHDRS-00001254
Title: Phytochemical and Biological Evaluation of Banana, Cantaloupe and Guava Waste Parts
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Background: This work aimed to study the lipoidal matter, phytoconstituents, total polyphenols (TP), total flavonoids (TF) and total carotenes (TC) contents of peels of both Musa paradisicae L. var. sapientum Kuntze (banana) and Cucumis melo L. (cantaloupe) in addition to pulp of Psidium guajava L. (guava) cultivated in Egypt and evaluate their antioxidant, antimicrobial and anticancer activities. Methods: Lipoidal matters were prepared and analyzed by GC/MS according to British pharmacopeia. TP, TF and TC of 80% hydro-methanolic extracts of the parts under investigation were detected by colorimetric methods. The isolated compounds were identified by different physico-chemical methods including UV, HPLC, 1H NMR and ESI-MS. Results: Palmitic acid was the main saturated fatty acid in the three parts and the percentage of sterols, in banana peel only was detected as 72.47% higher than the percentage of hydrocarbons (23.58%). Twenty compounds were isolated and identified by different physical and chromatographic techniques (7 compounds from banana peels, 5 compounds from cantaloupe peels and 8 compounds from guava pulp). Extract of banana peel showed the best antimicrobial activity while extract of guava pulp showed the best activity against all tested tumor cell lines. Conclusion: Thus, waste parts of the investigated fruits showed a significant medicinal value for their active compounds.

Cite this article as:Mona E. El-Tantawy, Eman G. Haggag, Amel M. Kamal, Rasha M. Lithy,Phytochemical and Biological Evaluation of Banana, Cantaloupe and Guava Waste Parts,Journal of Pharmacy Research 2016,10(5),308-318.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PB-00001241
Title: Effect of Pullulan concentration in fast dissolving films formulation and exploration of film properties
Category: Pharmaceutical Biotechnology
Section: Research Article
Country: India
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Background: Dissolvable oral thin films made from biopolymers gain much attention by pharmaceutical industries due to their fast dissolution capabilities. Objective: To cast the pullulan based film with plasticizer and silver nitrate and to explore the film properties for appearance, thickness, and disintegration and dissolution times for various films. Materials and methods: Aqueous solutions of pullulan (1-5%), 1-5 % of poly ethylene glycol (400, 600 varieties) and 1- 10 mM silver nitrate were formulated into films by solvent casting method. Thickness was measured by screw gauge and disintegration and dissolution times were physically observed and reported. Results: Formulated films were clear transparent, translucent and opaque in color. Least values of disintegration (15 sec) and dissolution (37 sec) times were observed and increase in the concentration of polymer and plasticizers increased the disintegration and dissolution times. Conclusion: The prepared pullulan based films can be used as fast dissolving films for systemic delivery of active pharmaceutical ingredients (APIs).

Cite this article as: V S Rama Krishna Ganduri, N Meher Reethika, M Jayasai, V Sirisha, S Vasudha, Sudhakar Poda,Effect of Pullulan concentration in fast dissolving films formulation and exploration of film properties,Journal of Pharmacy Research 2016,10(5),211-215.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-P'Col-00001247
Title: Effect of Aspirin and Celecoxib on Lens Glutathione and Soluble Protein Profile in Naphthalene-Induced Cataract in Wistar Rats
Category: Pharmacology
Section: Research Article
Country: India
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Background: Opacification of eye lens causing cataract, has become a leading cause of blindness worldwide. Aspirin, a non selective COX inhibitor and Celecoxib, a NSAID and selective COX II inhibitor, have been evaluated for their anti-cataract effect using naphthalene cataract model. Methods: Adult female albino rats of Wistar strain weighing between 180 and 230 grams were taken and divided into six groups. Group I received light liquid paraffin 5 ml/kg/day p.o. for 6 weeks. Group II received naphthalene solution 0.5 gm/kg/day p.o. for first three days and 1 gm/kg/day p.o. thereafter for six weeks. Group III received Aspirin suspension at the dose of 100 mg/kg/day p.o. along with naphthalene. Group IV received Celecoxib suspension at the dose of 34 mg/kg/day p.o. along with naphthalene. Group V received Aspirin suspension alone at the dose of 100 mg/kg/day p.o. Group VI received Celecoxib suspension alone at the dose of 34 mg/kg/day p.o. Drug suspensions were made every day by 1% carboxy methyl cellulose (CMC) in 25 mM sodium phosphate buffer, pH 7.4. All the above groups were treated for 42 days. Cataract was examined by torchlight and ophthalmoscope. Cataract was developed in all animals. On the 43rd day lenses were removed from the eyes of all groups of rat for estimation of lens glutathione and lens soluble protein. Results and discussion: Naphthalene administered to the rats reacted via a metabolite 1, 2-naphthequinone which caused oxidative damage primarily to the lens proteins located in the cortex and decreased the cellular oxidants level. The fact that decreases in reduced glutathione (GSH), the earliest biochemical changes observed, has suggested that oxidative stress may be the primary mechanism of damage in this model. With the increase in oxidative stress created by 1, 2- naphthoquinone, the protein gets denatured and forms disulfide cross-linkages leading to disulfide and mixed disulfide bond formation, hence protein aggregation, precipitation and lens opalescence. Our test drugs have increased the GSH and soluble proteins level thereby shown protection towards cataract. Conclusion: Lens glutathione and soluble protein profile showed the preventive role of Aspirin and Celecoxib in naphthalene-induced cataract in female rats.

Cite this article as: Syed Ehtaishamul Haque and Mohammad Tauseef,Effect of Aspirin and Celecoxib on Lens Glutathione and Soluble Protein Profile in Naphthalene-Induced Cataract in Wistar Rats,Journal of Pharmacy Research 2016,10(5),270-274.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-P'Col-00001253
Title: Amelioration of gastrotoxic effect of indomethacin by piperine in male Wistar rats: a novel therapeutic approach
Category: Pharmacology
Section: Research Article
Country: India
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Background: Gastric ulcer, an alarming disease among middle aged person in India and middle East Asia, generally develops due to long term use of Non-Steroidal Anti Inflammatory Drugs (NSAIDs), consumed as pain killers. It is well established that oxidative stress and free radical mediated injury play a pivotal role in generation of gastric ulcer. Antioxidant activity and gastro protective effects of black pepper are well known for long time. Piperine, a major alkaloid present in black pepper also possesses free radical scavenging activity. Objective: The aim of this study is to evaluate the ameliorative role of piperine against indomethacin, a classical NSAID, induced gastric damage. Methods: Indomethacin was orally administered in male albino Wistar rats to generate gastric ulcers. These rats were orally fed with graded doses of aqueous solution of piperine prior to indomethacin administration. Oxidative stress biomarkers, activities of antioxidant and pro-oxidant enzymes, PGE2 level, activities of mitochondrial enzymes and histology of gastric tissues were studied. Results: From the obtained data, it was seen that, Indomethacin treatment altered all the above mentioned parameters whereas, piperine pre-treated animals were protected against indomrthacin induced ulceration. Conclusion: Hence, piperine possess the potentiality to be used as an antiulcer drug against indomethacin induced gastric ulcer or can be used as an co-therapeutic agent among those patients undergoing NSAID treatment.

Cite this article as:Nirajan Ghosal, Syed Benazir Firdaus, Sudeshna Paul, Shamreen Naaz, Aindrila Chattopadhyay, Prachi Shukla, Garima Jain, Sanjib Pattari, Vinod D. Rangari and Debasish Bandyopadhyay,Amelioration of gastrotoxic effect of indomethacin by piperine in male Wistar rats: a novel therapeutic approach,Journal of Pharmacy Research 2016,10(5),240-254.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-MB-00001239
Title: Antimicrobial activity of some medicinal plant extracts and its synergistic interaction with some antibiotics
Category: Microbiology
Section: Research Article
Country: India
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Background: The synergism is a new concept in developing agents for antimicrobial therapy. The new approach is combination therapy i. e. combination of different plant extracts with antibiotics against resistant microorganisms which may lead to new ways of treating infectious diseases. Synergistic interaction means when two agents are combined together and they exert an inhibitory effect that is greater than the sum of their individual effects. The present study focuses on the in vitro antimicrobial activity and synergistic activity of various parts of different plants such Aegle marmelos, Annona squamosa, Citrus limon, Piper betle, and Azadiracta indica. Material and Method: The synergistic antimicrobial activity was evaluated by the Agar disc diffusion method against Gram- positive, Gram -negative and Fungi. Result and Discussion: All the plant extracts with Gentamicin showed good synergistic antibacterial activity against Gram-positive and Gram-negative bacteria. All the plant extracts with Ketoconazol showed good synergistic antifungal activity against fungi. Conclusion: Hence, these plants extracts can be used as good medicinal sources in combination with antibiotics to treat infectious diseases.

Cite this article as:Hemali Padalia, Anjali Trivedi, Sumitra Chanda,Antimicrobial activity of some medicinal plant extracts and its synergistic interaction with some antibiotics, Journal of Pharmacy Research 2016,10(5),211-220.

 

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-CPRMS-00001246
Title: Storage and Disposal of Medicines in Home -A Review
Category: Clinical Pharmacy and Related Medical Science
Section: Review Article
Country: India
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Appropriate household storage and use of drug products reduce drug wastage and unnecessary hazards. Rational use of drug also focuses on the storage and disposal of drugs irrespective of both Over the Counter (OTC) and prescription drugs. At present, pharmacy practice is in developing stage and focuses on drug related issues. Therefore, the awareness and importance of storage and disposal of drugs among consumers remained/s underreported and less focused topic. Pharmacist has prime responsibility to make aware people regarding storage and disposal of medicine.

Cite this article as: Patel Denis Pankajkumar, Sneha Chacko, Bhatt Sandipkumar Prakashkumar,Storage and Disposal of Medicines in Home -A Review,Journal of Pharmacy Research 2016,10(5),290-295.

 

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PHDRS-00001252
Title: Studies on Phytochemical Screening of Leaf Fraction of Lantana camara Linn.
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Background: The study was conducted to determine the antimicrobial activity of varying concentrations of Lantana camara Linn. leaf extracts against Staphylococcus aureus, Bacillus subtilis and Escherichia coli. Methods: Broth microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was evaluated through streaking of bacterial suspension onto Mueller-Hinton agar. Phytochemical tests were done to identify bioactive compounds present in the extracts. Results and Discussion: The results showed that among extracts used, L.camara leaf ethanolic fraction (EF) demonstrate best antibacterial activity. The MIC of ethanolic fraction ranges from at 1,250μg/mL – 5,000μg/mL. Bacillus subtilis is the most sensitive organism inhibited at 312.5 ìg/mL. The MIC-MBC ratio revealed that the extracts possess bactericidal activity against test bacteria. Phytochemical evaluation indicates the presence of saponins, tannins and terpenoids in EF. Conclusion: L.camara leaf ethanolic fraction showed antibacterial activity and contain phytochemicals responsible for the broad antibacterial spectrum of the plant which may lead to new drug development.

Cite this article as: Preeti Caesar, Jainendra Kumar,Studies on Phytochemical Screening of Leaf Fraction of Lantana camara Linn.,Journal of Pharmacy Research 2016,10(5),304-307.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PSPA-00001228
Title: Phytochemical Evaluation and Antiinflammatory Effects of Hymenodictyon excelsum (Roxb.) Wall Grown in Uttarakhand State of India
Category: Phytochemical Study and Pharmacological Activity
Section: Editorial
Country: India
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Background: A number of medicinal plants have traditionally been used as potent anti-inflammatory drug. The present study was aimed at phytochemical evaluation and assessment of anti-inflammatory activity of bark of Hymenodictyon excelsum (Roxb.) Wall., a deciduous tree in the Himalayan region of India. Methods: Different extracts of the bark were qualitatively evaluated for presence and/or absence of different types of phytochemicals. Physicochemical evaluation and TLC profiling of extracts was carried out to develop standard parameters for authentication. The anti-inflammatory activity of the methanolic extract was carried out by egg albumin induced rat paw edema model. Results: The phytochemical evaluation showed the presence of highest number of secondary metabolites in the methanol extract. The tested plant exhibited significant anti-inflammatory activity comparable to the standard anti-inflammatory drug Piroxicam. Conclusion: The study led to the conclusion that the bark of H. excelsum possesses promising anti-inflammatory efficacy. Results of physicochemical and phytochemical studies provided the scientific basis to bioefficacy of the plant. However, further research is needed on the active ingredient of the plant responsible for anti-inflammatory and other biological activity.

Cite this article as :Paramita Chakraborty, Y. C. Tripathi, Devesh Tewari, Lokesh Upadhyay,Phytochemical Evaluation and Antiinflammatory Effects of Hymenodictyon excelsum (Roxb.) Wall Grown in Uttarakhand State of India.,Journal of Pharmacy Research 2016,10(5),199-204.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PHDRS-00001245
Title: Phytochemical and pharmacognostic evaluation of leaf and stem extracts of Gynochthodes ridsdalei Razafim & B. Bremer (Rubiaceae): An endemic medicinal plant of southern Western Ghats
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Background: Phytochemistry describes the large number  of secondary metabolic compounds found in plants. Pharmacognosy describes the discovery of new drugs or as an aid for plant  physiology studies. Methods: The powdered leaves and stem of Gynochthodes ridsdalei ( Syn: Morinda reticulata) were used for physicochemical, fluorescent analysis and different solvent extracts of leaf and stem were used for phytochemical screening. Results and Discussion: The results of physicochemical parameters such as moisture content, fibre content, total ash, acid insoluble ash, water soluble ash, sulphated ash, alcohol soluble extractives  and water extractive values were interpreted in the tables  followed by the results of fluorescent analysis and phytochemical analysis. Conclusion: The present investigation on the pharmacognostic and phytochemical evaluation serve as a tool to identify and determine the quality and purity of the plant material in future and the phytochemicals  present in the plant which is having many medicinal properties.

Cite this article as: Renji  Ramachandran  Nair, Gangaprasad Appukuttan Nair,Phytochemical and pharmacognostic evaluation of  leaf and stem extracts of Gynochthodes ridsdalei Razafim & B. Bremer  (Rubiaceae): An endemic medicinal plant of southern Western Ghats,Journal of Pharmacy Research 2016,10(5),233-239.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PC-00001251
Title: Hybrid Triazoles: Design and Synthesis as Potential Dual Inhibitor of Growth and Efflux Inhibition in Methicillin Resistant Staphylococcus aureus.
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Background: Staphylococcus aureus, the bacteria on the verge of eradication once, is now a great threat to mankind due to resistance. Emergence of methicillin-resistant Staphylococcus aureus (MRSA) & vancomycine-resistant Staphylococcus aureus (VRSA) have reaffirmed their dominance in the area of infections. However, new antibacterial drugs with new mechanisms of action have not been developed in over forty years. Apart from mutation, efflux of drug by bacteria is one of major way for drug resistance. Methods and Materials: Efflux inhibitors (EI) have been tested recently as adjuvant therapy to treat MRSA & VRSA. Toxicity and untoward side effects, limits wide use of these EI to treat MRSA & VRSA. Here we report a series of compounds containing linked triazoles to hybrid molecules derived from known efflux inhibitor. Synthesized molecules were evaluated as growth (GI) and Efflux inhibitor of TB initially with S. aureus. Pharmacologically active compounds were then tested for their cytotoxicity to further narrow down search. Results & Discussion: Most active compounds 107, 121, 132 & 134 were then tested for their GEI action against methicillin-resistant S. aureus. Said compounds were also tested for their synergistic action with first line and second line anti-TB drugs and ethidium bromide (EtBr). Conclusion: We herein report compound 135 as most potent dual inhibitor of MRSA.

Cite this article as : Prasad P. Dixit, Prashant P. Dixit, Bharat D. Dond, Shivajirao N. Thore,Hybrid Triazoles: Design and Synthesis as Potential Dual Inhibitor of Growth and Efflux Inhibition in Methicillin Resistant Staphylococcus aureus.Journal of Pharmacy Research 2016,10(5),275-289.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PC-00001238
Title: Synthesis, characterization and anti-microbial activities of novel N-[6-fluoro-7-substituted-1,3-benzothiazol-2-yl]-4-[3-chloro-2-(nitrophenyl)-4-oxo-azetidin-1-yl]benzenesulfonamides.
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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A series of novel N-[6-fluoro-7-substituted-1,3-benzothiazol-2-yl]-4-[3-chloro-2-(3-nitrophenyl)-4-oxo-azetidin-1-yl]benzenesulfonamide 6-17 and N-[6-fluoro-7-substituted-1,3-benzothiazol-2-yl]-4-[3-chloro-2-(4-nitrophenyl)-4-oxo-azetidin-1yl] benzenesulfonamide 18-29 derivatives were synthesized using appropriate synthetic route. Their structures were characterized by elemental analysis, UV, IR and 1HNMR. The entire test compounds 6-29 were screened for anti-microbial activity which exhibited good to moderate activity.

Cite this article as:Vijay Jagtap, Rashmi Mahabal, Vinod Mule, Sandip Murtale and E. Jayachandran,Synthesis, characterization and anti-microbial activities of novel N-[6-fluoro-7-substituted-1,3-benzothiazol-2-yl]-4-[3-chloro-2-(nitrophenyl)-4-oxo-azetidin-1-yl]benzenesulfonamides,Journal of Pharmacy Research 2016,10(5),191-198.

 

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PB-00001244
Title: Studies on the Anti-fibrotic potential of Phyllanthus maderaspatensis Linn. hexane extract on human activated hepatic stellate cell line- LX-2
Category: Pharmaceutical Biotechnology
Section: Research Article
Country: India
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Background: Hepatic fibrosis has become a serious life threatening condition, without effective therapy till date. This condition is characterized by increased deposition of Extra Cellular Matrix proteins (collagens, proteoglycans, fibronectin etc.), secreted by activated Hepatic Stellate Cells (HSCs) and hepatic scarring. Methods: In the present study, we used activated LX-2 cells that mimic HSCs functionally. To evaluate the in vitro anti fibrotic activity of the promising extract , the various methodologies adopted in this study are immunofluorescence, MTT , Morphological studies, and Hoechst staining. Results and Discussion: Our findings showed that Phyllanthus maderaspatensis Linn. hexane extract not only inhibits the proliferation of activated LX-2 cell (IC50– 200µg/ml for 48 hours or 100 µg/ml 72 hours), but also suppressed the fibrotic gene expression (as evidenced by the inhibition of α-Smooth muscle actin- the marker- expression) along with functional restoration. Our study showed that Phyllanthus maderaspatensis Linn. hexane extract has good anti-fibrotic potential. Conclusion: Through the preliminary in vitro screening studies, the anti-fibrotic potential of Phyllanthus maderaspatensis Linn. hexane extract has been demonstrated confirmedly, but demands further studies towards identification of the mechanism of action of the extract, which is underway.

Cite this article as: K.A. Krishnakumar, Sheena Philip, Greeshma Tom , V.V.Asha, Studies on the Anti-fibrotic potential of Phyllanthus maderaspatensis Linn. hexane extract on human activated hepatic stellate cell line- LX-2, Journal of Pharmacy Research 2016,10(5),205-210.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-MB-00001250
Title: In-vitro Antibacterial Activity in Different Extracts of Lantana camara L. Leaf Fraction
Category: Microbiology
Section: Research Article
Country: India
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Background: Medicinal use of plants is the oldest form of healthcare known to mankind. India has a rich profusion of medicinal plants and 75% of its folk population is still using herbal preparations. Present study reports about antimicrobial potentiality of Lantana camara as its leaf extracts exhibit good antimicrobial, fungicidal, insecticidal and nematicidal properties and the plant might be a novel source of antimicrobial drug. Methods: Two enteropathogens as (A) - B. subtilis (positive, obligate aerobic) and (B) - E. coli (Gram-negative, facultative anaerobic) were used for the present study. Four solvent phases viz, methanol, ethanol, acetone and aqueous were used for extraction of antimicrobial agent. The screening of antimicrobial property was done by well diffusion method. Results and Discussion: Leaves of Lantana camara showed excellent antibacterial activity in all the solvent phases used against both E. coli as well as B. subtilis. Lantana is effective against both the bacteria. The aqueous extract showed minimum ineffective antimicrobial activity against E. coli and B. subtilis, where as acetone phase showed maximum activity against B. subtilis and ethanol against E. coli as shown in the terms of maximum zone of inhibition. Conclusion: The leaves of Lantana camara linn. proved to be good antibacterial agent as they show antagonistic effect on the growth of both tested microbes in all the solvent phases used. Large scale production of its active constituents would be an advantageous for natural, herbal antimicrobial agents.

Cite this article as: Preeti Caesar and Dr. Jainendra Kumar,In-vitro Antibacterial Activity in Different Extracts of Lantana camara L. Leaf Fraction,Journal of Pharmacy Research 2016,10(5),301-303.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-ND-00001237
Title: Assessment of Variation in Rhoifolin Content in Aerial Parts of Uraria picta Desv. from Different Locations of Madhya Pradesh
Category: Natural Drugs
Section: Research Article
Country: India
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Background: Uraria picta Desv. is one of the ingredient of ten herbs formulation called Dashmoola. Rhoifolin is an active compound which has been isolated from the aerial parts of Uraria picta and has been established as marker compound for quality evaluation of this species. In the present time of global resurgence in traditional and alternative health care systems, the quality assurance of medicinal plants is very essential. Since, WHO and herbal Pharmacopoeia are also emphasizing on quality standardization of medicinal plants in terms of active ingredients, the present study was aimed to assess the variation in rhoifolin content in aerial parts of Uraria picta collected from eight locations of Madhya Pradesh for its quality standardization and selection of superior chemotype. Methods: The powdered samples of aerial parts were extracted by soxhlet extraction in methanol for 4 hours on water bath. Extracts were quantified for rhoifolin content using Ethyl acetate: Formic acid: Glacial acetic acid: Water (10: 1.1: 1.1: 2.6) mobile phase and a CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner, WIN CATS software Version 1.4.8 and Hamilton Syringe (100 µL). Results: Rhoifolin content varied from 0.039% to 0.195% among the aerial part samples of eight locations corresponding to seven agroclimatic regions of the state. The highest content was found in aerial parts of Pandado, Sehore (0.25%) followed by Kapoornala, Chhindwara (0.167%) and Pamakhedi, Khandwa (0.152%) respectively and lowest content in plant sample of Navali, Mandsaur (0.039%). Conclusions: The study provided significant variation in rhoifolin content in aerial parts of Uraria picta. The accession collected from Pandado, Sehore emerged out of best quality on the basis of rhoifolin content and superior chemotype too.

Cite this article as:Hari Om Saxena, Brij Mohan and Arun Kakkar,Assessment of Variation in Rhoifolin Content in Aerial Parts of Uraria picta Desv. from Different Locations of Madhya Pradesh,Journal of Pharmacy Research 2016,10(5),185-190.

 

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PC-00001243
Title: To study toxic potential of Melia azedarach, Euphorbia helioscopia and Sapium sabiferum leaves extracts against Cimex lectularius
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Objectives: The aim of the research work was to find out the toxic potential of Melia azedarach, Euphorbia helioscopia and Sapium sabiferum. C. lectularius or bed bugsare the ectoparasites of bats, man, chicken and rarely other animals, which are cosmopolitan in distribution. They reduce the caliber of human life by triggering banishment, discomfort, nervousness and restlessness.Bed bugs show resistance to Insecticides such as DDT, lindane, malathion, carbaryl, permethrin and d-phenothrin. Newly, researchers take countlessconcern in the use of plant extracts as a new control substitute to synthetic insecticides. Methods : In present study, toxicprospective of three local plants, Melia azedarach, Euphorbia helioscopia, and Sapium sebiferum against Cimex lectularius was determined. Plant samples were collected from territory of Abdul Wali Khan University, Mardan. For determination of toxic activity of these plants, 20 bed bugs were placed in each petri dish having filter paper saturated in different concentrations (100ppm, 200ppm, 300ppm) of water and chloroform extracts of leaves. In many choice experiment percent mortality of bed bugs was calculated after 12, 24 and 48 hrs. Results: Maximum mortality rate of Melia azedarach, E. helioscopia,and S. sebiferum was observed after 48 hours for all three plants in water solvent (10.34, 12.34and 14.34 %, 5.67, 7.67and 10.34%, 5.67, 8.00and 10.34 % correspondingly) as well as in chloroform solvent (9.67, 12.67 and 15.67 %, 8.67, 10.34 and 14.67 %, 8.34, 9.34 and 11.67 % respectively). Conclusion: Among three solvent extracts tested, the maximum activity was observed in both water and chloroform extracts of Melia azedarach against Cimex lectularius.

Cite this article as: Sumbal Haleem, Sadaf Niaz, Shahid Niaz Khan, Hameed Ur Rehman, Shazab Sajad, Naveeda Akhtar Qureshi,To study toxic potential of Melia azedarach, Euphorbia helioscopia and Sapium sabiferum leaves extracts against Cimex lectularius,Journal of Pharmacy Research 2016,10(5),226-232.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PC-00001249
Title: Design and Synthesis of Hybrid Triazoles as Potential Dual Inhibitor of Growth and Efflux in Methicillin-Resistant Staphylococcus aureus
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Background: Efflux inhibition is proven bacterial machinery responsible for removal of bacterial wastage including antibiotics. Recently, efflux inhibitors (EI) have been tested with encouraging results as an adjuvant therapy for treatment of various bacterial invasions especially methicilline-resistant Staphylococcus aureus (MRSA). Although, EI have emerged as innovative approach of treatment for several multi drug resistant bacterial infections including tuberculosis, toxicity profile limits their wider use. Materials and Methods: To address this issue, we have attempted synthesizing hybrid molecules those results by combining known EI and triazole. This synthesis was aimed to arrive at structure that possesses pharmacophore from known EI. Synthesized molecules were evaluated as growth inhibitors (GI) and Efflux inhibitor of S. aureus. Pharmacologically active compounds were then tested for their cytotoxicity to further narrow down search. Results and Discussion: Most active compounds 144, 145, 154 and 163 were then tested for their GEI action against MRSA. Synthesized compounds were also tested for their synergistic action with first line and second line antibacterial drugs and ethidium bromide (EtBr). Conclusion: We arrived at compound 145 as most potent dual inhibitor.

Cite this article as : Prasad P. Dixit, Prashant P. Dixit, Amol B Kale, Shivajirao N. Thore,Design and Synthesis of Hybrid Triazoles as Potential Dual Inhibitor of Growth and Efflux in Methicillin-Resistant Staphylococcus aureus,Journal of Pharmacy Research 2016,10(5),255-269.

 

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PHDRS-00001235
Title: Detection of adulterated Radix valerian herb by FTIR spectroscopy and extraction solvent optimization for HPTLC fingerprinting
Category: Pharmacognosy and Herbal Drugs related study
Section: Editorial
Country: India
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Background: Radix valerian is a natural preparation of the root and rhizome of the perennial herb utilized primarily in the treatment of insomnia and sleep disturbances. Quality control of valerian represents a major concern in the pharmaceutical industry due to the huge variety of these species and their almost daily new scientific findings. Objective: Our objective was to investigate the sensitivity of FTIR spectroscopy for the detection of adulterated Radix valerian and optimization of a solvent extraction method for HPTLC fingerprinting. Methods: Radix valerian medicinal plant adulterant was measured by FTIR spectroscopy. The obtained data were evaluated using SIMCA (soft independent modeling of class analogies) in order to determine the detection limits of adulterants with a dataset comprising 50 batches and HPTLC system was utilized to evaluate optimal solvent composition consisting of methanol, water and dichloromethane for which the flavonoid fingerprint is determined. Results: With regard to the chemometric analysis of the FTIR spectra we found that for this particular dataset the overall detection rates computed from leave-one-out cross-validation were 56.2%, 85.9% and 98.3% at 5%, 10% and 20% contamination levels, respectively. Solvent extraction optimization, we found that a mixture of 40-80% Methanol, 30-50% Dichloromethane and 10-30% water was most efficient for the analysis of the flavonoid patterns in the six plant systems under investigation. Conclusion: Concerning the extraction optimization, a universal solvent could not be defined exactly by response surface methodologies as reported in similar studies involving different analytical platforms mostly by HPLC but suggestions for near optimal solvent compositions with competitive performances compared to pure and 70% methanol could be made.

Cite this article as: Praveen Kumar Vemuri, Bhavana Talluri, VBSC Thunuguntla, Suryanarayana Veeravalli, Vijaya Lakshmi Bodiga,Detection of adulterated Radix valerian herb by FTIR spectroscopy and extraction solvent optimization for HPTLC fingerprinting, Journal of Pharmacy Research 2016,10(5),181-184.

 

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PCS-00001242
Title: Formulation development and evaluation of Carbamazepine fast dissolving tablets
Category: Pharmaceutics
Section: Research Article
Country: India
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Background: The main objective of present research work is to formulate the Carbamazepine Fast Dissoving tablets. Carbamazepine, an antiepileptic, belongs to BCS Class-II and used to control some types of seizures in the treatment of epilepsy and Neuropathic Pain by blocking use-dependent sodium channels. Methods: The Fast Dissoving tablets of Carbamazepine were prepared employing different concentrations of Crospovidone and Croscarmellose sodium in different combinations as a Sperdisintegrants by Direct Compression technique using 32 factorial design. The concentration of Crospovidone and Croscarmellose sodium was selected as independent variables, X1 and X2 respectively whereas, wetting time, Disintegration time, t50% ,t90%were selected as dependent variables. Results and Discussion: Totally nine formulations were designed and are evaluated for hardness, friability, thickness, Assay, Wetting time, Disintegration time, In-vitro drug release. From the Results concluded that all the formulation were found to be with in the Pharmacopoeial limits and the In-vitro dissolution profiles of all formulations were fitted in to different Kinetic models, the statistical parameters like intercept (a), slope (b) & regression coefficient (r) were calculated. Polynomial equations were developed for Wetting time, Disintegration time, t50%, t90%. Validity of developed polynomial equations were verified by designing 2 check point formulations (C1, C2). According to SUPAC guidelines the formulation (F5) containing combination of 9.375% Crospovidone and 9.375% Croscarmellose, is the most similar formulation (similarity factor f2=82.675, dissimilarity factor f1= 2.049 & No significant difference, t= 0.041) to marketed product (TEGRETOL-100). Conclusion: The selected formulation (F5) follows First order, Higuchi’s kinetics, mechanism of drug release was found to be Non-Fickian Diffusion (n= 0.665).

Cite this article as: Raghavendra Kumar Gunda, J. N. Suresh Kumar, V. Satyanarayana, Swathi Batta, Ch . Meher Harika,Formulation development and evaluation of Carbamazepine  fast dissolving tablets,Journal of Pharmacy Research 2016,10(5),216-225.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: May
Article Id: JPRS-PB-00001248
Title: Sequential Heating and Solvent Precipitation For Effective Pullulan Recovery From Jaggery Based Fermentation Broths
Category: Pharmaceutical Biotechnology
Section: Research Article
Country: India
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Background: Cost-effective downstream processing is very important aspect in successful fermentative production of pullulan biopolymer. Objective: To study the heat treatment method for removal of proteins in cell free supernatant and to optimize the pullulan concentration using several solvents in terms of type, solvent ratio and precipitation time for their efficacy. Materials and methods: Fermentation broths obtained from sucrose and jaggery based shake flasks fermentations were exposed to heat at 50 to 1100C for 5 to 60 min to remove proteins and precipitated with organic solvents like ethanol, methanol, acetone, isopropanol, chloroform and diethyl ether. Various effects such as supernatant: solvent ratio, precipitation time were analyzed. Results: For jaggery (50 g/L) fermentation broth, the optimum heating temperature and time were found at 1100C and 50-60 min, respectively. Addition of Isopropanol, solvent ratio of 1:3 and 12 hours of precipitation time at 40C were observed for maximum pullulan concentration from precipitation. Conclusion: These sequential steps may enhance efficiency of pullulan recovery and would become effective downstream processing method.

Cite this article as:V S Rama Krishna Ganduri, Srinivasulu K, Usha Kiranmayi M, Bodaiah B, Y V V Aswani Kumar, Vijaya Lakshmi M, Sudhakar Poda, Sequential Heating and Solvent Precipitation For Effective Pullulan Recovery From Jaggery Based Fermentation Broths,Journal of Pharmacy Research 2016,10(5),296-300.