Journal Menu

Issues

Journal of Pharmacy Research
ISSN NO: 0974-6943
The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified when, we/or author get comments from any readers. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry,  Nursing, Paramedical, prescription etc  fields).
Scopus Indexed (link http://www.scimagojr.com/journalsearch.php?q=21100325431&tip=sid&clean=0) 

Journal Metrics for this Journal of Pharmacy Research (Source ID: 21100325431): 2015(SNIP) Source Normalized Impact Per Paper : 0.575; SCImago Journal Rank (SJR):0.787; Impact Per Publication : 0.789 CiteScore 2016: 0.93(Top level : Life Science)

Year SJR Cites per document Year Value
2014 0.607 Cites / Doc. (4 years) 2014 0.607
2015 0.787 Cites / Doc. (4 years) 2015 0.789
2016 0.926 Cites / Doc. (4 years) 2016 0.926
    Cites / Doc. (3 years) 2014 0.607
    Cites / Doc. (3 years) 2015 0.789
    Cites / Doc. (2 years) 2014 0.607
    Cites / Doc. (2 years) 2015 0.789
2016   Cites / Doc. (4 years) 2016 0.926
Cites Year Value
External Cites per document 2014 0.607
External Cites per document 2015 0.789
External Cites per document 2016 0.926
Cites per document 2014 0.607
Cites per document 2015 0.789
Cites per document 2016 0.926

Manuscripts Published

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PB-00001315
Title: Promoter hypermethylation of Tumor Suppressor Genes in Oral Squamous Cell Carcinoma
Category: Pharmaceutical Biotechnology
Section: Review Article
Country: India
View Article

The oral squamous cell carcinoma (OSCC) is the common cancer subtype in the world causing millions of deaths. The detection and analysis of these oral lesions are done through traditional biopsy detection method, which is painful and time taking. The major reason for the oral cancer were found to be smoking and tobacco chewing despite of the several other reasons which are responsible for cancer. Epigenetic changes are one of those changes which occurs due to environment and dietary factors. These epigenetic changes are reversible and can be detected through MS-PCR. Various tumor suppressor genes get hypermethylated cytosine and are in turn inactivated. These changes leads to the downregulation or silencing of the gene which leads to cancer. These tumor suppressor genes may play vital role in the diagnosis of cancer and provide better alternative as a diagnostic biomarker.

Cite this article as: Shantanu Gupta , Sandesh Kumar Patel , Meenakshi Jha, Anju Shrivastava , Abhimanyu Kumar Jha,Promoter hypermethylation of Tumor Suppressor Genes in Oral Squamous Cell Carcinoma,Journal of Pharmacy Research 2016,10(6),426-430.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PHDRS-00001300
Title: Pharmacognostic studies on Persicaria odorata (Lour.) Sojak
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
View Article

Background: Persicaria odorata (Lour.) Sojak (Family: Polygonaceae), commonly known as ‘Vietnamese coriander’ or ‘Daun Laksa’ in Malaysia, often used in culinary and salads, is an important medicinal plant having its application in the traditional system of medicine in treating swelling and inflammation, diarrhoea and excessive bleeding, sores, ulcers and wounds. Earlier reported biological activities of the plant include algaecide,antidiabetic, antibacterial and antifungal, antioxidant and anticancer activities. In spite of several uses of this plant, there are no reports on the pharmacognostic studies about this plant in the literature. Methods: In the present paper, we report some pharmacognostic studies on the leaves using standard recommended procedures. Results and Discussion: The transverse section of the leaves showed the characteristics of a dorsiventral leaf with numerous oil glands present in the mesophyll region. Presence of uniseriate multicellular covering trichomes and calcium oxalate crystals (rosettes) were identified in the powder microscopy. The preliminary phytochemical screening of different extracts revealed presence of steroids, terpenoids, flavonoids, tannins and phenolic compounds, carbohydrates, mucilages, proteins and amino acids respectively in the plant. Conclusion: The findings of the study can be useful in establishing pharmacognostic standards for the plant.

Cite this article as:Gouri Kumar Dash, Zahida Binti Zakaria,Pharmacognostic studies on Persicaria odorata (Lour.) Sojak,Journal of Pharmacy Research 2016,10(6),377-380.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PB-00001314
Title: Green synthesis of silver nanaoparticles from leaf extract of Cascabela thevetia, physicochemical characterisation and antimicrobial activity
Category: Pharmaceutical Biotechnology
Section: Research Article
Country: India
View Article

Background: Since there is a great commercial demand for biosynthesis of silver nanoparticles (AgNPs) which have wide applications in medicine and agriculture, the present work biosynthesis of AgNPs have been designed Objective: The present study involves the synthesis, characterisation and antimicrobial activity of Cascabela thevetia leaf extract mediated AgNPs. Materials and methods: The synthesized AgNPs were characterized by UV-visible spectrophotometer, X-Ray diffractometer (XRD), Fourier transform infra red analysis (FTIR), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM). Antimicrobial activity of the green synthesized nanoparticles was tested against Gram positive bacteria, Gram negative bacteria and selected pathogenic fungi Results: The leaf mediated silver nanoparticles has shown a single absorption spectrum at 438nm in UV-Visible spectrophotometer. The synthesized green AgNPs are crystalline in nature and mostly spherical in shape with an average size of 14.35nm. AgNPs have shown significant activity against both Gram positive and Gram negative bacteria as well as selected pathogenic fungi. Conclusion: The AgNPs of Cascabela thevetia have exhibited antimicrobial property on selected human pathogenic bacteria and fungi.

Cite this article as: Bodaiah Bonigala, Usha Kiranmayi M, Vijayalakshmi M, KRS Sambasiva Rao, Ravi Varma A, Sudhakar Poda,Green synthesis of silver nanaoparticles from leaf extract of Cascabela thevetia, physicochemical characterisation and antimicrobial activity,Journal of Pharmacy Research 2016,10(6),410-418.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-P'Col-00001285
Title: Gastroprotective effect of Fenugreek 4-hydroxyisoleucine and trigonelline enriched fraction (TF4H (28%)) Sugaheal® against indomethacin induced ulcer in male wistar rats.
Category: Pharmacology
Section: Editorial
Country: India
View Article

Background: Sugaheal®, the 4-hydroxyisoleucine and trigonelline enriched fraction (TF4H (28%)) isolated from the seed of Trigonella foenum graecum (Linn.) is a well established antidiabetic drug. Several experiments have revealed that Sugaheal® also possesses good antioxidant characteristics. Gastric ulcer generally develops due to long term use of Non-Steroidal Anti Inflammatory Drugs (NSAIDs), consumed as pain killers. It is well established that oxidative stress and free radical mediated injury play a major role in generation of gastric ulcer. This influenced us to investigate the ameliorative role, if any, of Sugaheal® against indomethacin (IMN), a classical NSAID, induced gastric damage. Objective: Evaluation of the protective effect of Sugaheal® against indomethacin induced gastric ulceration. Methods: Indomethacin was orally administered in male Wistar rats to generate gastric ulcers. These rats were orally fed with aqueous solution of Sugaheal® prior to indomethacin administration. Oxidative stress biomarkers, activities of antioxidant enzymes, activities of mitochondrial enzymes and gastric tissue morphology were studied through histological analysis. Results: From those obtained data, it was seen that, indomethacin treatment altered all the above mentioned parameters whereas; Sugaheal® pre-treatment prevented those deleterious changes. Biochemical and histological data supported these findings. Conclusion: Hence it can be said that besides being a potent anti-diabetic drug, Sugaheal® also possess the potentiality to be used as an antiulcer drug against indomethacin induced gastric ulcer.

Cite this article as : Nirajan Ghosal, Syed Benazir Firdaus, Shamreen Naaz, Sudeshna Paul, Arnab Kumar Ghosh, Aindrila Chattopadhyay, Vishwaraman Mohan, Prasad Thakurdesai, Sunil Bhaskaran, Sanjib Pattari, Debasish Bandyopadhyay, Gastroprotective effect of Fenugreek 4-hydroxyisoleucine and trigonelline enriched fraction (TF4H (28%)) Sugaheal® against indomethacin induced ulcer in male wistar rats, Journal of Pharmacy Research 2016,10(6),351-364.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-P'Col-00001319
Title: Orally administered aqueous bark extract of Terminalia arjuna protects against adrenaline-induced myocardial injury in rat heart through antioxidant mechanisms: an in vivo and an in vitro study
Category: Pharmacology
Section: Research Article
Country: India
View Article

Objective: The present study is aimed to evaluate the cardioprotective effects of the most effective dose of aqueous bark extract of Terminalia arjuna (TA) on adrenaline- bitartrate induced myocardial damages in male albino rats. Methods: After sacrifice of rats, the left ventricular portion of heart tissues were used for determination of biomarkers of oxidative stress, activities of antioxidant enzymes, Kreb’s cycle enzymes and respiratory chain enzymes by using standard methods. Results: Treatment of rats with adrenaline - bitartrate induced alterations in the activities of serum lactate dehydrogenase total (LDH -T), lactate dehydrogenase-1(LDH -1), serum glutamate oxaloacetate transaminase (SGOT), tissue and serum nitric oxide (NO) concentration. Moreover, it caused elevation in the level of lipid peroxidation and protein carbonylation, a decrease in glutathione content as well as altered the activities of antioxidant enzymes and the enzymes of Kreb’s cycle and respiratory chain. Tissue histomorphological studies also showed considerable damage following adrenaline treatment. Pre-treatment of rats with aqueous bark extract of TA significantly protected against these myocardial damages. Conclusion: The present studies suggest that the effective dose of aqueous bark extract of TA may be beneficial in ameliorating adrenaline-induced oxidative stress mediated myocardial injury.

Cite this article as: Sanatan Mishra , Shamreen Naaz , Arnab K. Ghosh, Sudeshna Paul, Nirajan Ghosal, Mousumi Dutta, Debasish Bandyopadhyay, Aindrila Chattopadhyay,Orally administered aqueous bark extract of Terminalia arjuna protects against adrenaline-induced myocardial injury in rat heart through antioxidant mechanisms: an in vivo and an in vitro study, Journal of Pharmacy Research 2016,10(6),454-478.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PCS-00001326
Title: Development and validation of RP-HPLC method for estimation of Tapentadol hydrochloride
Category: Pharmaceutics
Section: Research Article
Country: India
View Article

OBJECTIVE: To develop a simple, novel, sensitive, precise and specific RP-HPLC method for the determination of Tapentadol hydrochloride. METHOD: The chromatographic separation was achieved on C18 column (250 mm × 4.6 mm inner diameter, 5 μm particle size) as a stationary phase using potassium dihydrogen phosphate (0.2M):Methanol (pH 5, adjusted with ortho phosphoric acid) as mobile phase in 40: 60 ratio at detection wavelength 215 nm in an isocratic mode at a flow rate of 1 ml/min. RESULTS: The calibration curve for tapentadol hydrochloride was linear from 1 to 50 μg/ml. The correlation coefficient (r2) value was found to be 0.9908. Precision study showed % RSD value less than 2% in all selected concentrations. The % recoveries of tapentadol hydrochloride are in the range of 100.49-102.85 %. The limit of detection and limit of quantification for Tapentadol hydrochloride were found to be 1.4827 and 0.4893 μg/ml respectively. CONCLUSION: Considering the specifications of this method, the system was found to be suitable for rapid, direct routine analysis and stability of tapentadol in bulk drugs.

Cite this article as: Nikunja B Pati*, Vinyas Mayasa, Swapna Velivela, Dr. Gupta VRM,Development and validation of RP-HPLC method for estimation of Tapentadol hydrochloride,Journal of Pharmacy Research 2016,10(6),479-483.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PC-00001289
Title: Characterization of AuNPs in enhancing efficiency of quantitative Polymerase Chain Reaction [qPCR]
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
View Article

Background: Gold nanoparticles (AuNPs) are useful material in diagnostic applications. AuNPs provide attractive feature for quantitative polymerase chain reaction (qPCR) enhancement ability because they have excellent heat transfer property. In this study, we evaluated the role of AuNPs in improving the efficiency of PCR using complement Factor H related protein 1 (cfhr1) as gene of interest. Methods: AuNPs were prepared following a previously reported citrate reduction protocol in M.A. Hayat book with slight modifications using chloroauric acid as source of gold. Results and discussion: We found that the sensitivity of qPCR technique was enhanced with the help of AuNPs. We have standardized the appropriate size (51.51 and 33.2 nm) and concentration (0.7 nM) of AuNPs, to optimize the qPCR assay for cfhr1 gene detection. We find that the PCR sensitivity is modulated by the size of the AuNPs. It is observed that, AuNPs of larger sizes are less efficient as compared to lower size. Conclusions: We also state that AuNPs can cause PCR inhibition when added at higher concentration in PCR master mix. These results demonstrate that appropriate concentrations and size of AuNPs seems to be the key factor of enhance qPCR.

Cite this article as: Bahadur Singh Gurjar, Satyajit Rath, Anita Kamra Verma,Characterization of AuNPs in enhancing efficiency of quantitative Polymerase Chain Reaction [qPCR],Journal of Pharmacy Research 2016,10(6),327-333.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PCS-00001312
Title: Development and Evaluation of Solid Self Double Emulsifying Drug Delivery System(SSDEDDS) : A Novel Approach to Enhance Bioavailability of BCS Class III Drugs
Category: Pharmaceutics
Section: Research Article
Country: India
View Article

Background: Even after having a good therapeutic importance few drugs may not be utilize effectively because they may lack either in solubility or permeability. BCS class III drugs have high solubility but low permeability thus bioavailability of these drugs is always low. The present study is based on the development of Self Double Emulsifying Drug Delivery System (SDEDDS) to enhance bioavailability of such drugs. Methods: Four formulation of the w/o emulsions was developed by one step emulsification procedure containing a fixed proportion of ranitidine and different amount of phospholipids. Ranitidine was dissolved in distilled water the pH was adjusted to 6.0±0.5 by a pH meter. Then, the drug aqueous solution was added to the oil phase which consisted with oleic acid, Span 80 and bean phospholipids under moderate magnetic stirring. Hard gelatine capsules (size 00) were manually filled with 890 mg of each formulation (F1–F5), resulting in each capsule containing 40 mg ranitidine. Results: Formulations were evaluated for weight variation, drug content, viscosity, globule size, visual grading, Dissolution & stability. The prepared formulation were complies the test as per the standard. The dissolution study revealed the sustained release of ranitidine from the formulation and enhances in vitro bioavailability. Conclusion: The present studies have clearly demonstrated the potential utility of SDEDDS for formulating ranitidine with sustained release in vitro and improved oral bioavailability in vivo. The SDEDDS readily released the lipid phase to form fine water-in-oil-in-water double emulsions, with a sustained release of ranitidine.

Cite this article as:Sachin M. Kolhe, Arun T. Patil, Pradeep P. Bawane, Jineetkumar B. Gawad,Development and Evaluation of Solid Self Double Emulsifying Drug Delivery System(SSDEDDS) : A Novel Approach to Enhance Bioavailability of BCS Class III Drugs,Journal of Pharmacy Research 2016,10(6),403-409.

 

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PC-00001324
Title: Multidrug Resistance Reversal Acridones: Pharmacophore Modeling and 3D QSAR Studies
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
View Article

In the current investigations, we have performed pharmacophore modeling and built a 3D QSAR model for a series of substituted acridone derivativeswith multidrug resistance reversal activity. An efficient pharmacophore has been identified from a data set of 32 molecules and the identified pharmacophore hypothesis consisted of two hydrogen bond acceptors, one hydrogen bond donor, and three aromatic rings i.e., AADRRR. A powerful 3D-QSAR model has also been constructed by employing Partial Least Square regression analysis with a regression coefficient of 0.95 (R2) and Q2 of 0.79, and Pearson-R of 0.84.

Cite this article as:M Amareswararao, Y Rajesh Babu, VVSR Prasad,Multidrug Resistance Reversal Acridones: Pharmacophore Modeling and 3D QSAR Studies,Journal of Pharmacy Research 2016,10(6),450-453.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PC-00001311
Title: Synthesis, and anti-cancer activity of some novel 3-(piperazin-1-yl)pyrazin-2-amine derivatives
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
View Article

Aim: Synthesis of some new 2-aminopyrazine derivatives for the preliminary studies on cytotoxicity against breast cancer cells. Methods: The starting materials for the synthesis of new 2-aminopyrazine derivatives (PP1-18) are aryl-piperazines and 3,5-dibromo-2-aminopyrazine , which were prepared by the known literature procedure and condensed to get the new derivatives. The structure of newly synthesized compounds were characterized by spectral data and studied for their cytoxicity activity against breast cancer lines. Results: Some compounds showed moderate cytotoxicity on breast cancer cells and some have non toxic. Conclusions: In this article we reported the synthesis of a new (PP1-18) 2-aminopyrazine derivatives. The cytotoxicity studies showed that compounds were moderately toxic and some are non-toxic Hence the fact that the compounds prepared in this study are chemically new and not related to current medication and further work is clearly warranted.

Cite this article as:Sadagopan Sekar, Nagaraja Naik, Ajjanna M Sridhara , Shivanna Yogish,Synthesis, and anti-cancer activity of some novel 3-(piperazin-1-yl)pyrazin-2-amine derivatives,Journal of Pharmacy Research 2016,10(6),419-425.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-CPRMS-00001282
Title: Storage and Disposal of Medicines In Home Among Students
Category: Clinical Pharmacy and Related Medical Science
Section: Research Article
Country: India
View Article

Background: The awareness and importance of storage and disposal of drugs among consumers remained/s underreported and less focused topic. Therefore, attempt found essential and made to explore storage and disposal of medicine at home in society. Purpose: To study storage and disposal of medicines in home among students. Method: This was an observational, prospective self-administered cross sectional survey conducted among students of the Pharmacy, Nursing, Arts and Commerce College, Kadi Sarva Vishwavidyalaya (KSV), Sector 23, Gandhinagar. The survey was conducted with help of predefined questionnaire in pre and post education session. Only close-ended questions were designed to get the required information with maximum possible options in short period of time. Finally a leaflet (summary form) with instructions for storage and disposal of medicine in home was provided to students. Result: A total of 372 respondents replied usable responses in our study. The mean age of respondents was 21 years. The most of students rated common places of storage were kitchen, bedroom and bathroom. The respondents used to throw their unwanted or expired medication in dustbin as they were. Conclusion: We conclude that knowledge about storage and disposal at home among students were inadequate. Drug-use assessments are necessary to increase their awareness about safe and proper storage and disposal methods.

Cite this article as: Patel Denis Pankajkumar, Sneha Chacko, Bhatt Sandipkumar Prakashkumar, Deshpande Srikalp S.,Storage and Disposal of Medicines In Home Among Students,Journal of Pharmacy Research 2016,10(6),343-350.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-P'Col-00001309
Title: Melatonin protects against lead acetate induced oxidative stress-mediated changes in morphology and metabolic status in rat red blood cells: a flow cytometric and biochemical analysis
Category: Pharmacology
Section: Research Article
Country: India
View Article

Background: Lead is an abundantly occurring heavy metal known to be toxic in higher quantities for humans and other animals. Moreover, lead exposure has previously been shown to cause damage to red blood cell. However, a detailed study with a plausible mechanism is still lacking. The pineal hormone, melatonin, is well known for its antioxidant and free radical scavenging properties. Thus, we examined the effect of melatonin pre-treatment on lead acetate-induced toxicity in rat RBCs in vivo as well as in vitro and tried to find out the mechanism behind such protection. Main methods: Rats were injected intra-peritoneally with lead acetate (15mg/kg/day) for seven consecutive days in presence/absence of melatonin (10mg/kg body weight). RBCs isolated from whole blood were haemolysed and was used for measuring changes in biochemical parameters and altered red blood cell morphology. Results & Discussion: Rats injected intra-peritoneally with lead acetate (15mg/kg/day) for seven days exhibited an altered status of lipid peroxidation level, reduced glutathione content, protein carbonyl and oxidized glutathione levels along with inhibition of superoxide dismutase and catalase activities in RBCs indicating generation of oxidative stress. Data obtained using light microscopy, scanning electron microscopy and flow cytometry indicate deterioration of RBC morphology along with marked alterations in granularity. Atomic absorption spectrophotometric analysis revealed lead accumulation and reduction in iron and zinc levels along with decreased carbonic anhydrase and met haemoglobin reductase activities in RBCs isolated from lead acetate-treated rats. An increase in osmotic fragility and marked changes in the activities of the glutathione metabolising enzymes, hexokinase, glucose-6-phosphate dehydrogenase, aldolase, lactate dehydrogenase and acetylcholine esterase in RBC were also observed following lead acetate treatment. Rats pre-treated with melatonin (10mg/kg body weight) displayed restoration of these altered activities, suggesting its ameliorative action against lead acetate toxicity. Additionally, in vitro studies indicated that lead acetate-induced alterations of RBC enzyme activities are time and concentration-dependent and when co-incubated with melatonin, these changes were restored. Conclusion: The present study demonstrates the potential ability of melatonin to provide protection against lead acetate-induced injury to RBCs through its antioxidant properties in addition to removal of non-competitive inhibition of some of the enzymes.

Cite this article as: Debosree Ghosh, Sudeshna Paul, Shamreen Naaz, Debajit Bhowmik, Mousumi Dutta, Arnab K. Ghosh, Syed Benazir Firdaus, Aindrila Chattopadhyay, Russel J. Reiter , Debasish Bandyopadhyay,Melatonin protects against lead acetate induced oxidative stress-mediated changes in morphology and metabolic status in rat red blood cells: a flow cytometric and biochemical analysis,Journal of Pharmacy Research, 2016,10(6),381-402.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-P'Col-00001318
Title: Anti ulcer potential of ethanolic extract of Syzygium alternifolium leaves on Albino rats.
Category: Pharmacology
Section: Research Article
Country: India
View Article

Background: The fruit juice of Syzygium alternifolium is used to cure stomach ache and ulcers, while fruit pulp and alcoholic extract of seeds posses anti-diabetic properties. The leaves of plants are claimed for anti-ulcer properties by folklore medicine. Objective: To investigate the antiulcer activity of Ethanolic extract of Syzygium alternifolium (EESA) leaves by Pylorus ligation (Shay rat model), stress ulcers by cold water immersion, Indomethacin induced ulcers, ethanol induced mucosal damage (cytoprotective activity) in albino rats. Materials and methods: The different concentrations of EESA 1gm/kg and 2gm/kg and Ranitidine as standard 150mg/kg and saline (control) were administered orally to albino rats (n=6 animals per group). The antiulcer effect was investigated by measuring ulcer index, determination of total acidity, acid volume and pH of gastric juice. Results: The EESA showed significant (p<0.001) reduction in the ulcer index, reduction in the volume of gastric contents. Total acidity and PH was increased as compared to control group in all the models. Conclusion: The results of the study reveal that the EESA has antiulcer activity which supports the claims in folklore medicine.

Cite this article as: D. Priyadarshini, Saroj Kumar Sahoo*, G. Soundarya, Ch. Kishore Kumar, K. Usha Rani,Anti ulcer potential of ethanolic extract of Syzygium alternifolium leaves on Albino rats,Journal of Pharmacy Research 2016,10(6),442-449.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-P'Col-00001274
Title: Evaluation of antiatherosclerotic and antihyperlipidemic effect of Sirolimus and Mycophenolate mofetil in high fat diet induced atherosclerosis in rats
Category: Pharmacology
Section: Research Article
Country: India
View Article

Background: Atherosclerosis is a chronic inflammation of the vascular wall typified by the accumulation of lipid and macrophage derived foam cells. As the endothelial cells become activated and adhesive, monocytes stick to them, pass between them, and enter the intimal layer of the vessel wall. With the use of Immunomodulators. Majority of immunomodulators like Sirolimus and Mycophenolate mofetil are being used in renal transplant patients. Some of clinical evidences indicated that use of immunomodulators causes hyperlipidemia and increases the chances of atherogenesis. Whereas in preclinical studies various researchers reported that immunomodulators reduces the chances of atherogenesis. So because of non uniform results in clinical and preclinical studies, this study was designed with a purpose of evaluating role of Sirolimus and Mycophenolate mofetil in atherosclerosis. Methods: Sirolimus and Mycophenolate mofetil at 3 different doses have been evaluated in High Fat diet (1% Cholesterol) induced atherosclerosis in rats. Atorvastatin 2 mg/kg was used as standard treatment. Up to day 28 High fat diet was administered and then from day 29 to day 56 treatment and High fat diet was administered. Biochemical estimations of Serum lipid profile, C Reactive Protein, Malondialdehyde and histopathology of aorta was performed. Results and Discussion: There was significant reduction in Triglyceride, LDL cholesterol, Total Cholesterol, Atherogenic Index, HMG to mavelonate, C reactive protein and MDA level was observed with treatment of Cyclosporine and ashwagandha. There was significant elevation in HDL cholesterol level and was observed with treatment of Sirolimus and Mycophenolate mofetil. Conclusion: Sirolimus and Mycophenolate mofetil effectively reduce the risk of development of atherosclerosis.

Cite this article as: Gajjar A.V., Deshpande S.S.,Evaluation of antiatherosclerotic and antihyperlipidemic effect of Sirolimus and Mycophenolate mofetil in high fat diet induced atherosclerosis in rats,Journal of Pharmacy Research 2016,10(6),319-326.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PHDRS-00001304
Title: In vitro anti-inflammatory screening of stem bark of Cordia obliqua Willd. in raw 264.7 cell lines
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
View Article

Background:Inflammation is a protective response that is initiated either after injury, physical and chemical damage, or infection by microorganism, but persistent inflammation may cause chronic disesases. The present study was made to assess the in vitro anti-inflammatory activity of ethanolic extract of stem bark of Cordia obliqua Willd. The plant belongs to family Boraginaceae, is medium sized deciduous tree. The traditional uses of bark are anthelmintic, constipating, cooling, depurative and used in vitiated conditions of kapha, pitta and leprosy. Method: Antiinflammatory studies were conducted by analyzing inhibition of cycloxygenase(COX), 5-lipoxygenase(5-LOX), myeloperoxidase and cellular nitrite levels on cultured RAW 264.7 cell lines and compared with standard diclofenac sodium. Estimation of secondary metabolites like flavanoids, and carotenoids were conducted. Antioxidant activity screening of the extract was also carried out by DPPH, nitric oxide and iron chelating assays. Result and Discussion: IC 50 value of the extract by COX/ 5-LOX inhibition was found out as 84.32µg/ml, 21.26 µg/ml respectively. Diclofenac showed COX/ 5-LOX inhibitions at the IC 50 values of 2.84 &12.14 µg/ml. The extract showed its ability to reduce myeloperoxidase and cellular nitrite levels also. Antioxidant activity screening showed that the extract is a potent source of antioxidants. Results revealed that the extracts are having anti-inflammatory activity, by inhibiting COX, 5-LOX, myeloperoxidase and nitric oxide due to the presence of flavanoids and carotenoids through antioxidation. Mechanism of action of the traditional claim of C.obliqua bark may be due to the COX/ 5-LOX inhibition, and the dual inhibition can reduce the common adverse effects of NSAIDs. Conclusion: The present study demonstrates that stem bark of Cordia obliqua Willd is a potent anti-inflammatory agent.

Cite this article as: Bindu AR, NA Aleykutty, Jyoti Harindran,In vitro anti-inflammatory screening of stem bark of Cordia obliqua Willd. in raw 264.7 cell lines,Journal of Pharmacy Research 2016,10(6),370-376.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PC-00001316
Title: Design, synthesis and anti-malarial activity of coumarin fused quinoline derivatives
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
View Article

Novel coumarin containing quinoline derivatives were synthesized by convergent synthesis in which quinoline derivatives and coumarin were separately synthesized and finally fused together, 2-cholro-3-formyl quinoline derivatives were synthesized by reacting various substituted acetanilide with Vilsmeier-Haack reagent (DMF & POCl3). Later 3-acetyl coumarin was synthesized by treating salicylaldehyde with ethyl acetoacetate in the presence of piperidine using grindstone method. Finally 3-acetyl coumarin and 2-cholro-3-formyl quinoline derivatives were condensed by dissolving in ethanol in presence of 40% KOH. This reaction was called as crossed Aldol or Clasein Schimdt condensation and it was done by microwave assisted technique. The formed product was called as Chalcones. All synthesized compounds were identified by melting point and TLC, as well as characterized by IR, 1H-NMR spectroscopy and Mass spectrometry. The synthesized and characterized compounds were screened for anti-malarial activity. The anti-malarial activity of selected derivatives was performed by In vitro schizonticidal testing method (ISM) using plasmodium falciparum as test organism and chloroquine phosphate as standard. Results revealed that some compounds showed equipotent anti-malarial activity and rest of the compounds showed mild to moderate when compared to the standard. Based on the results, it had been concluded that the derivative 5d showed effective anti-malarial activity due to the presence of nitro group at para position compared to other derivatives.

Cite this article as:Geetha Pushpalatha , N. Pramod , G. Mahaboob Basha, M. Deepa, P. Neelaphar, BHM Jayakumar swamy,Design, synthesis and anti-malarial activity of coumarin fused quinoline derivatives,Journal of Pharmacy Research 2016,10(6),437-441.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-P'Col-00001273
Title: Evaluation of antiatherosclerotic and antihyperlipidemic effect of Cyclosporine and Ashwagandha in high fat diet induced atherosclerosis in rats
Category: Pharmacology
Section: Research Article
Country: India
View Article

Background: Atherosclerosis is a chronic inflammation of the vascular wall typified by the accumulation of lipid and macrophage derived foam cells. As the endothelial cells become activated and adhesive, monocytes stick to them, pass between them, and enter the intimal layer of the vessel wall. With the use of Immunomodulators. Majority of immunomodulators like cyclosporine, Sirolimus and Mycophenolate mofetil are being used in renal transplant patients. Some of clinical evidences indicated that use of immunomodulators causes hyperlipidemia and increases the chances of atherogenesis. Whereas in preclinical studies various researchers reported that immunomodulators reduces the chances of atherogenesis. So because of non uniform results in clinical and preclinical studies, this study was designed with a purpose of evaluating role of cyclosporine and ashwagandha in atherosclerosis. Method: Cyclosporine and Ashwagandha at 3 different doses have been evaluated in High Fat diet (1% Cholesterol) induced atherosclerosis in rats. Atorvastatin 2 mg/kg was used as standard treatment. Up to day 28 High fat diet was administered and then from day 29 to day 56 treatment and High fat diet was administered. Biochemical estimations of Serum lipid profile, C Reactive Protein, Malondialdehyde and histopathology of aorta was performed. Results: There was significant reduction in Triglyceride, LDL cholesterol, Total Cholesterol, Atherogenic Index, HMG to mavelonate, C reactive protein and MDA level was observed with treatment of Cyclosporine and ashwagandha. There was significant elevation in HDL cholesterol level and was observed with treatment of Cyclosporine and ashwagandha. Conclusion: Cyclosporine and ashwagandha effectively reduce the risk of development of atherosclerosis.

Cite this article as: Gajjar A.V., Deshpande S.S.,Evaluation of antiatherosclerotic and antihyperlipidemic effect of Cyclosporine and Ashwagandha in high fat diet induced atherosclerosis in rats,Journal of Pharmacy Research 2016,10(5),334-342.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 6.
Article Id: JPRS-PCS-00001301
Title: Formulation and Evaluation of Taste Masked Spherical Agglomerates of Telmisartan
Category: Pharmaceutics
Section: Research Article
Country: India
View Article

Oral administration of bitter drugs, using novel solid dosage forms essentially require an acceptable degree of taste masking. Telmisartan is chosen as the model drug as it is bitter in taste and has poor water solubility and low bioavailability. Telmisartan is an orally active non peptide angiotensin II receptor antagonist belonging to BCS class II with an aqueous solubility of 9.9 µg/ml and log P of 7.7. One of the major formulation problems with this drug is the bitter taste which gives rise to patient compliance problems when it is given as conventional dosage forms. In the present study taste masking of telmisartan was achieved by preparing taste masked spherical agglomerates using a pH-sensitive polymer, Eudragit RS-100, and polymers like chitosan and carbopol. Taste assessment of these prepared agglomerates was done by both spectrophotometric taste evaluation technique and panel testing. Compressed tablets of taste masked agglomerates which rapidly disintegrated in the oral cavity were prepared using oxidized starch as directly compressible filler and sodium starch glycolate as a super-disintegrant. These were subsequently evaluated for various pharmacopoeial tests, drug release, and disintegration time in the oral cavity. Sensory taste evaluation was carried by panel testing in 20 healthy human volunteers. Results indicate successful formulation of taste masked oral fast disintegrating tablets which disintegrated in the oral cavity in about 30 s and possessed good taste.

Cite this article as:Varinder Soni,Gagan Shah,Sandeep Rahar,Shaina Sharma, Formulation and Evaluation of Taste Masked Spherical Agglomerates of Telmisartan,Journal of Pharmacy Research 2016,10(6),365-369.