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Journal of Pharmacy Research
ISSN NO: 0974-6943
The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified when, we/or author get comments from any readers. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry,  Nursing, Paramedical, prescription etc  fields).
Scopus Indexed (link http://www.scimagojr.com/journalsearch.php?q=21100325431&tip=sid&clean=0) 

Journal Metrics for this Journal of Pharmacy Research (Source ID: 21100325431): 2015(SNIP) Source Normalized Impact Per Paper : 0.575; SCImago Journal Rank (SJR):0.787; Impact Per Publication : 0.789 CiteScore 2016: 0.93(Top level : Life Science)

Year SJR Cites per document Year Value
2014 0.607 Cites / Doc. (4 years) 2014 0.607
2015 0.787 Cites / Doc. (4 years) 2015 0.789
2016 0.926 Cites / Doc. (4 years) 2016 0.926
    Cites / Doc. (3 years) 2014 0.607
    Cites / Doc. (3 years) 2015 0.789
    Cites / Doc. (2 years) 2014 0.607
    Cites / Doc. (2 years) 2015 0.789
2016   Cites / Doc. (4 years) 2016 0.926
Cites Year Value
External Cites per document 2014 0.607
External Cites per document 2015 0.789
External Cites per document 2016 0.926
Cites per document 2014 0.607
Cites per document 2015 0.789
Cites per document 2016 0.926

Manuscripts Published

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 7.
Article Id: JPRS-MB-00001374
Title: Antibacterial and antioxidant activities and phytochemical screening of aqueous methanol extracts of eight Nigerian medicinal and aromatic Plants
Category: Microbiology
Section: Research Article
Country: India
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Objective: This study investigates the antibacterial activity, antioxidant potential and major classes of phytochemicals of eight Nigerian plant parts used in combination by traditional healers in Nigeria to treat cancer. Methods: The antibacterial activity was estimated by determination of the zone of inhibition and minimum inhibitory concentrations (MICs) against fourteen species of bacteria. Results: The results indicated that the Xylopia aethiopica extract possessed the highest activity, active against 7 of the 10 Gram-positive bacterial strains and 3 Gram-negative bacteria (Proteus vulgaris, Klebsiella pneumoniea and Escherichia coli), with minimum inhibitory concentrations ranging from 0.313 to 1.0 mg/ml. The phytochemical screening revealed varied distribution of bioactive compounds such as alkaloids, tannins, saponins, flavonoids, terpenoids, and steroids in the powdered samples and their methanol extracts. In the antioxidant assay, Globimetula oreophila extract showed the strongest DPPH scavenging activity with an IC50 value of 0.38 ± 0.03 mg mL-1 and the highest FRAP value of 1100 ± 10.63 µmol. Fe2+ /g . Conclusions: This preliminary study shows that tested extracts demonstrated some level of antioxidant and antibacterial effects, thus suggesting that the plant parts may be used, after further characterization of active constituents and in-vivo studies, as possible natural antioxidant and antimicrobial agents to control various human diseases.

Cite this article as: Esther O. Faboro, Wisut Wichitnithad, Olatomide A. Fadare, David A. Akinpelu, Craig A. Obafemi, Antibacterial and antioxidant activities and phytochemical screening of aqueous methanol extracts of eight Nigerian medicinal and aromatic Plants,Journal of Pharmacy Research 2016,10(7),523-532.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 7.
Article Id: JPRS-PCS-00001327
Title: Formulation and Evaluation of Sildenafil Citrate Fast Dissolving Tablets Using Crospovidone and Croscarmellose Sodium Superdisintegrants
Category: Pharmaceutics
Section: Research Article
Country: India
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Aim: Sildenafil citrate is the drug of choice for the treatment of erectile dysfunction. Bioavailability of Sildenafil citrate is 41% of orally administered dose. The objective of the present study was to develop fast dissolving tablets of Sildenafil citrate and improve its bioavailability, reduce the dosing frequency and thereby maximizing the therapeutic effect of the drug. Method: Sildenafil citrate fast dissolving tablets were prepared by wet granulation method using a combination of superdisintegrants, crospovidone and croscarmellose sodium along with various other excipients in order to confer a therapeutic enhancement on the drug and provide organoleptic characteristics in the final dosage form. Compatibility of the drug with the excipients was investigated by FTIR studies. Fourteen formulations (F1-F14) were prepared and subjected to various pre-compression and post-compression parameters such as average weight, weight variation, hardness, thickness, friability, wetting time, in-vitro disintegration time, drug content, and in-vitro drug release. Results: The results of all the formulations were examined after subjecting to various evaluation parameters. Among all the formulations, batch F12 produced promising results in terms of very fast disintegration in 34.11 seconds and dissolving 100.6% in 45 minutes. Hence it was considered as the optimized formulation. Stability study and kinetic modeling were carried out on the optimized formulation, F12. The in-vitro disintegration time and in-vitro drug release of the optimized formulation were compared with the innovator product. Conclusion: Sildenafil citrate fast dissolving tablets were found to be a promising one when compared to the innovator product as they increase the bioavailability, rapid onset of action, avert the problems of swallowing and improve the patient compliance.

Cite this article as: Sandeep Kumar Reddy Adena, Kasi Viswanadh M, Ramya Krishna P, Anusha Induri, Formulation and Evaluation of Sildenafil Citrate Fast Dissolving Tablets Using Crospovidone and Croscarmellose Sodium Superdisintegrants,Journal of Pharmacy Research 2016,10(7),484-492.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 7.
Article Id: JPRS-PB-00001356
Title: Partial Purification, Characterization and Optimization of Anti-Leukemic Enzyme L-Asparaginase from Mangrove Soil Actinobacteria
Category: Pharmaceutical Biotechnology
Section: Research Article
Country: India
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Background: Marine biosphere is the earth’s richest actinobacterial habitat which is potent for the production of L-asparaginase, an enzyme that catalyzes the hydrolysis of L-asparagine. Method: An aggregate of 45 actinomycetes strains were isolated from a mangrove ecosystem situated in konaseema estuaries of Godavari River at Pallam village of Coastal Andhra Pradesh, of which 3 strains were recorded positive for the production of L-asparaginase. Isolation process was carried out using a selective ISP-2 medium. The potent strain was identified based on polyphasic taxonomical studies including 16S rDNA gene sequence analysis and designated as JRS-27. Optimization for maximum production of L-asparaginase was executed by stabilizing the physico-chemical parameters. Protein purification was done partially through ammonium sulphate precipitation followed by dialysis, gel filtration chromatography by Sephadex G-100 and Ion exchange chromatography by CM Sephadex C-50. Results: The partially purified L-asparaginase enzyme showed a specific activity of 670.04 IU/mg protein, 94.37 fold purity with a yield of 60%. The enzyme gives optimum activity in the culture medium with pH 8 and temperature 35°C as well as with different nitrogen and carbon sources. Conclusion: The enzyme produced was very specific for L-asparagine but not to L-glutamine. This is the first report on the production of L- asparaginase by JRS-27 from backwaters of konaseema mangroves.

Cite this article as: Ravi Varma A, Sushma Kanapala, Naga Sai Babu V, Bodaiah B and Sudhakar Poda,Partial Purification, Characterization and Optimization of Anti-Leukemic Enzyme L-Asparaginase from Mangrove Soil Actinobacteria,Journal of Pharmacy Research 2016,10(7),502-511.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 7.
Article Id: JPRS-P'Col-00001364
Title: Comparative Assessment of In-Vitro Anthelminthic Studies of Some Plants From Indian Origin
Category: Pharmacology
Section: Research Article
Country: India
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Objective: Helminthiasis is a now global problem mostly in poor countries. The main purpose of the present research is to investigate in-vitro anthelmintic activity of some plants of Indian origin. Methods: Different concentrations (25, 50, 100 mg/mL) of all the five plant (Areca catechu, Tamarindus indica Linn, Daucus Carota L., Nigella sativa Linn, Ocimum tenuiflorum ) extracts diluted with 3% Tween 80 in normal saline were used for the anthelmintic activity. Albendazole was used as a reference standard and normal saline has been treated as control. Results: The result was expressed in terms of time in minutes to report paralysis time and death time of earthworms. Ethanolic extracts of all the plant parts have exhibited a dose dependent inhibition of spontaneous motility i.e. Paralysis of Earthworms. At the concentration of 100mg/mL all the extracts except Daucus Carota L. showed significant result (p<0.05) against earthworms. Conclusions: The ethanolic extracts of all the five ethno botanicals have been supported their folklore use and possess the anthelmintic activity. The present research also established a more potent candidature of Ethanolic extracts of seed of Tamarindus indica Linn. as compared to others. This study can be extended for the isolation of condensed Tannin which is particularly responsible for the activity.

Cite this article as: Shambaditya Goswami, Kushal Nath Mishra, Ashutosh Mishra, Ajay Pratap Singh, Pradeep Singh , Prashant Singh,Comparative Assessment of In-Vitro Anthelminthic Studies of Some Plants From Indian Origin, Journal of Pharmacy Research 2016,10(7),514-518.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 7.
Article Id: JPRS-ND-00001345
Title: Isolation, Identification and anti oxidant and anti-inflammatory activities of Tridax Procumbens flower extracts
Category: Natural Drugs
Section: Research Article
Country: India
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Background: The present study was aimed to isolate Quercetin-3-O-α-L-rhamnopyranoside from flowers ethyl acetate extract of Tridax procumbens Linn for the first time. Methods: The structure of isolated bioactive compound was confirmed by using various spectral data like UV, FT-IR, 1H-NMR, 13C-NMR analysis. In vitro antioxidant activity of petroleum ether, ethyl acetate and methanol extracts was evaluated by using DPPH radical with reference to ascorbic acid. The results show that the petroleum ether, ethyl acetate and methanol extracts has a promising antioxidant activity measured by DPPH method with IC50 = 92.17 ± 0.01, 82.08 ± 0.60, 99.73 ± 0.20 respectively. Moreover, the anti-inflammatory activity of the petroleum ether, ethyl acetate and methanol extracts with reference to diclofenac sodium was evaluated in carrageenan induced hind paw edema. Results: These extract at dose of 200, 300 mg/kg produced significant inhibition of oedema by above mentioned methods. Conclusion: The results of the present investigation concluded that the flowers ethyl acetate extract of T. procumbens is a good source of Quercetin-3-O-α-L-rhamnopyranoside which harbors significant antioxidant and anti-inflammatory activities.

Cite this article as: R. Manivannan and S.Saravanan,Isolation, Identification and anti oxidant and anti-inflammatory activities of Tridax Procumbens flower extracts,Journal of Pharmacy Research 2016,10(7),502-506.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 7.
Article Id: JPRS-PB-00001340
Title: Molecular targets of Cinnamomum zeylanicum and its bio-active compound Cinnamaldehyde for anti-tumor activities against oral cancer
Category: Pharmaceutical Biotechnology
Section: Research Article
Country: India
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Background: Cinnamomum zeylanicum (CZE) and Cinnamaldehyde (Cin) which is its bioactive component have recently been exploited for their versatile therapeutic potentials including anti-diabetic and anti-inflammatory. Methods: Here, we demonstrated their elaborate anti-tumor activities in oral squamous cell carcinoma (OSCC) cell lines namely SSC-4 and KB. Tumor growth inhibition was studied by MTT assay and the effects on cell cycle and apoptosis by propidium iodide (PI)/annexin-V binding assay and subsequent flow cytometry. Finally, the different protein targets of these two compounds underlying these above cell processes were studied through western blotting. Results: We found that both CZE and Cin inhibited tumor cell proliferation in a dose dependent manner. The IC50 values were found to be 270 and 125 µg/ml for CZE) and 300 and 180 µM for Cin in SCC-4 and KB cell lines at 48 h respectively. Further, both CZE and Cin arrest tumor cell proliferation at G2/M phase of cell cycle by down-regulating cyclin D1 expression and induce apoptosis by up-regulating P53 protein along with inhibiting NF-κB. We also showed anti-angiogenic effects of CZE extracts on these cells as it minimizes vascular endothelial growth factor (VEGF) expression while no such effect was seen in case with Cin. Conclusion: Overall, we provide evidences that propose pharmaceutical implication of CZE extract and Cin for the prevention and/or treatment of oral cancer by targeting P53, cyclin D1, VEGF and NF-κB.

Cite this article as: Vijaya Bharti, Poonam Gaur, Satya N Das, Molecular targets of Cinnamomum zeylanicum and its bio-active compound Cinnamaldehyde for anti-tumor activities against oral cancer, Journal of Pharmacy Research 2016,10(7),493-501.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 7.
Article Id: JPRS-PCS-00001335
Title: Method Development and Validation of Acyclovir in Rabbit Plasma by RP-HPLC
Category: Pharmaceutics
Section: Research Article
Country: India
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Background:A simple, sensitive and rapid high performance liquid chromatographic method was developed and validated for Acyclovir from rabbit plasma. Method: Chromatographic separation and detection was carried out on a SHISEIDO C18 (250 x 4.6 mm, 5μ) column using 0.2M di-potassium hydrogenorthophosphate buffer pH 7 (pH 5 adjusted with ortho phosphoric acid) and Acetonitrile in theratio of 40:60with a flow rate of 1 ml/min at 254 nm. Results:Retention times of drug and Internal Standard (IS) were found out to be 4.05 min and 5.50 min respectively. The method was linear in the concentration range of 1-6mg/ml. The regression coefficient value was found to be 0.998. Conclusion: The proposed method was validated by performing linearity, recovery, specificity, robustness, LOD/LOQ and interday/intraday precision. The LOD and LOQ values were found to be 0.001093 and0.003012 μg/ml.

Cite this article as: Swapna Velivela, Konde Abbulu, Vinyas Mayasa, Nikunja B Pati, Method Development and Validation of Acyclovir in Rabbit Plasma by RP-HPLC,Journal of Pharmacy Research 2016,10(7),509-513.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 7.
Article Id: JPRS-P'Col-00001386
Title: Effect of Aspirin and Sorbitrate on Trace Element Status in the Patients of Ischemic Heart Disease and Myocardial Infraction
Category: Pharmacology
Section: Research Article
Country: India
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Aim: The effect of drugs aspirin and sorbitrate on the capability to change trace element level of serum and its relative change in enzyme activity for aspartate transaminase, alkaline phosphatase, ceruloplasmin and lactate dehydrogenase was analyzed for the patients of Ischemic heart disease (IHD) and myocardial infraction (MI). Material and Methods: Patients in the age group of 40 to 55 years were treated with the aspirin (300 mg) and sorbitrate (10 mg) and samples were analyzed along with control population for trace elements and enzyme activities. Results: With the treatment of the aspirin and sorbitrate no significant change in the trace element level was detected in both MI and IHD patients along with that no change was observed in the enzyme activity in treated patients even though the trace elements and enzyme activity remained abnormal when compared to control. Conclusion: Treatment of aspirin and sorbitrate patients of MI and IHD showed no significant effect on the trace element level along with enzyme activity but it has been recorded that enzymes remained dependent on trace elements for their activities in every condition.

Cite this article as: Vijay D. Gavhale, N R Akarte, Rajita A Lonare,Effect of Aspirin and Sorbitrate on Trace Element Status in the Patients of Ischemic Heart Disease and Myocardial Infraction,Journal of Pharmacy Research 2016,10(7),519-522.

Journal: Journal of Pharmacy Research , Volume: 10, Issue: 7.
Article Id: JPRS-ND-00001330
Title: Phytochemical Screening and Evaluation of Polyphenols, Flavonoids and Antioxidant Activity of Prunus cerasoides D. Don Leaves
Category: Natural Drugs
Section: Research Article
Country: India
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Background: Natural antioxidants have the ability to protect organisms from damage caused by free radical-induced oxidative stress. A lot of research has been carried out worldwide for finding natural antioxidants of plant origin. The present study was aimed to evaluate the phytochemical composition, total phenolic and flavonoid contents and antioxidant activity of P. cerasoides leaves. Methods: Extracts of P. cerasoides leaves were phytochemically examined for presence of different phytochemicals by standard methods. Total phenolic content of the various extracts was determined spectrometrically using Folin-Ciocalteu method, total flavonoid content by reported method and antioxidant efficacy by following DPPH radical scavenging protocol. Results: Phytochemical screening showed the presence of alkaloids, steroids, terpenoids, flavonoids, phenolics, tannins, saponins, glycosides, carbohydrate, protein and amino acids in different extracts of P. cerasoides leaves. Methanol extract recorded the maximum number of chemical constituents. Presence of flavonoids and phenolic constituents were mostly found in extracts obtained with solvents of medium and high polarity. The total phenolic content (6.26±0.033 mg GAE/g of extract), total flavonoid content (3.86±0.016 mg QE/g of extract) and free radical scavenging (antioxidant) activity (IC50, 56.00±0.242 μg/ml) were highest in methanol extract. Total phenolic content had positive correlation with antioxidant capacity. Conclusions: The present study established leaves of P. cerasoides as rich sources of phenolic compounds and natural antixodants. The study further provided scientific basis to traditional medicinal claims of the plant.

Cite this article as: Nino Joseph, Nishat Anjum and Y.C. Tripathi,Phytochemical Screening and Evaluation of Polyphenols, Flavonoids and Antioxidant Activity of Prunus cerasoides D. Don Leaves,Journal of Pharmacy Research 2016,10(7),502-508.