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Journal of Pharmacy Research
ISSN NO: 0974-6943
The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified when, we/or author get comments from any readers. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry,  Nursing, Paramedical, prescription etc  fields).
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Journal Metrics for this Journal of Pharmacy Research (Source ID: 21100325431): 2015(SNIP) Source Normalized Impact Per Paper : 0.575; SCImago Journal Rank (SJR):0.787; Impact Per Publication : 0.789 CiteScore 2016: 0.93(Top level : Life Science)

Year SJR Cites per document Year Value
2014 0.607 Cites / Doc. (4 years) 2014 0.607
2015 0.787 Cites / Doc. (4 years) 2015 0.789
2016 0.926 Cites / Doc. (4 years) 2016 0.926
    Cites / Doc. (3 years) 2014 0.607
    Cites / Doc. (3 years) 2015 0.789
    Cites / Doc. (2 years) 2014 0.607
    Cites / Doc. (2 years) 2015 0.789
2016   Cites / Doc. (4 years) 2016 0.926
Cites Year Value
External Cites per document 2014 0.607
External Cites per document 2015 0.789
External Cites per document 2016 0.926
Cites per document 2014 0.607
Cites per document 2015 0.789
Cites per document 2016 0.926

Manuscripts Published

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000496
Title: Antihyperlipidemic Activity of methanolic extract of Garlic (Allium sativum L.) in Triton X-100 induced hyperlipidemic rats
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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The present study is designed to evaluate the effect of garlic extract on lipid profiles in Triton X-100 induced hyperlipidemia in male wistar rats. Three fractional extracts (AS1, AS2 and AS3) were obtained by column chromatography from methanolic extract of garlic. All three garlic extract significantly increased (p < 0.0001) plasma HDL-Cholesterol and decreased plasma TC, LDL-Cholesterol and TG levels as compared with hyperlipidemic control. Among three fractions, AS3 has more significantly reduced the plasma lipid levels than others AS1and AS2.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000507
Title: Simultaneous Estimation of Atorvastatin and Ramipril by First Derivative Spectrophotometric method
Category: Pharmaceutics
Section: Research Article
Country: India
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A simple, precise and economical procedure for the simultaneous estimation of Atorvastatin (ATV) and Ramipril (RAM) in tablet formulation has been developed. ATV is a anticholesteremic agent as well as hydroxymethylglutaryl-CoA reductase inhibitor and RAM is a antihypertensive agent belonging to category of angiotensin-converting enzyme inhibitor. The formulation of Atorvastatin (ATV) and Ramipril (RAM) (Stator-R 5) in a combination mixture is very good pharmacologically since it enhances the efficacy of the individual drugs. The present study involving the simultaneous estimation done by first derivative method. ATV has absorbance maxima at 246nm in methanol and RAM has absorbance maxima at 208nm in methanol. Both these drugs obey Beer’s law in the concentration range employed for the present method. The result of analysis has been validated statistically by recovery studies. The slope and intercept for ATV were -0.00183 and -0.001and for RAM were 0.0036 and -0.046 respectively as determined by the method of least squares. The results were found satisfactory and reproducible. The method was applied successfully for the estimation of ATV and RAM simultaneously in tablet dosage form without the interference of common excipients.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000510
Title: Natural excipients in controlled drug delivery systems
Category: Pharmaceutics
Section: Review Article
Country: India
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The emergence of controlled release (CR) technology as an effective way to enhance patient compliance and extend the life cycle of a drug has led to the need for novel ways of controlling the drug release profiles. Polymers present a logical and simple approach to control the release of drugs. Natural and synthetic polymers serve as key excipients in oral and parenteral CR formulations. The use of natural excipients for controlled deliver of the bioactive agents has been hampered by the synthetic materials. However advantages offered by these natural excipients are their being non-toxic, less expensive and freely available. The performance of the excipients partly determines the quality of the medicines. The traditional concept of the excipients as any component other than the active substance has undergone a substantial evolution from an inert and cheap vehicle to an essential constituent of the formulation. This article gives an overview of natural excipients which are used in controlled drug delivery systems.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-P'Col-0000519
Title: Anti-asthmatic activity of methanolic extract of leaves of Tamarindus Indica Linn.
Category: Pharmacology
Section: Research Article
Country: India
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Tamarindus indica Linn. (Caesalpiniaceae), is a medicinal plant, used in folk medicine for treating asthma, dysentery, vaginal and uterine complaints, inflammation and variety of other condition. In this study methanolic extract of leaves of Tamarindus indica Linn at the doses of 175, 350 and 700 mg/kg, p.o., exhibited significant (p<0.01) mast cell stabilizing activity against clonidine induced mast cell degranulation in rats and significantly inhibited (p<0.01) the milk induced leucocytes and eosinophilia in mice. Clonidine, a α2 adrenoreceptors agonist induces dose dependent catalepsy in mice, which is inhibited by histamine H1 receptor antagonists. Prior treatment with methanolic extract of Tamarindus indica Linn at the dose of 250, 500 and 1000 mg/kg, p.o., significantly inhibited (p<0.01) the clonidine-induced catalepsy in mice. Thus the present study revealed that the methanolic extract of leaves of Tamarindus indica Linn exhibited significant antihistaminic, adaptogenic and mast cell stabilizing activity in the laboratory animals.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-P'Col-0000503
Title: Wound healing activity of the leaves of Tribulus terrestris (linn) aqueous extract in rats
Category: Pharmacology
Section: Research Article
Country: India
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The leaves of Tribulus terrestris(zygophyllaceae) are used traditionally in the folklore for the treatment of various kinds of wounds. The present study was undertaken to verify the effect of the leaves of T. terrestris on experimentally induced excision wound, incision wound and burn wound models in rats. Aloe vera was used as standard wound healing agent. A formulation of aqueous leaves extract in carbopol at 2.5% and 5% concentrations were prepared and applied to the wounds. In the excision and burn wound models, the animal showed significant reduction in period of epithelization and wound contraction (50%). In the incision wound model a significant increase in the breaking strength was observed. The results suggest that T. terrestris aqueous leaves extract applied topically possess wound healing activity.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000497
Title: The antiulcer activity of Pterospermum acerifolium bark extract in experimental animal
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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The role of ethanolic fraction of Pterospermum acerifolium bark extract on different experimental ulcer models in rats was investigated.The extract demonstrated significant antiulcer activity against aspirin, indomethacin & ethanol induced ulcerations, significant inhibition of gastric secretary volume, and total acidity in pylorus ligated rats were observed to occur with the extract.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-QA-0000523
Title: Validation of reverse phase high performance liquid chromatography method of metolazone and its determination in bulk drug and pharmaceutical dosage form
Category: Quality Assurance
Section: Research Article
Country: India
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To develop and validate a sensitive, selective, precise high performance liquid chromatographic (HPLC) method of analysis for metolazone both as bulk drug and formulations. A new sensitive and reproducible HPLC method was developed and validated for the determination of metolazone in tablets. The separation was achieved by using a JASCO HPLC system 2000 series comprising of JASCO PU 2080 plus pump, JASCO UV 2075 plus detector, Rheodyne manual injector fitted with 100 μl sample loop. The analytical column used was thermo C18 (250 X 4.6mm; 5μm) at ambient temperature using a mobile phase consisting of Acetonitrile: Water (50:50). The method was validated for accuracy, precision, linearity, reproducibility and robustness and statistical comparison was performed by analysis of variance. The developed HPLC technique is precise, specific, accurate and stable. Statistical analysis proves that the method is reproducible, selective and suitable to be applied for analysis of metolazone in commercial pharmaceutical dosage form for routine quality control application.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000509
Title: Phytochemical, pharmacological and phytopharmaceutics aspects of Sesbania grandiflora (Hadga) : A review
Category: Pharmacognosy and Herbal Drugs related study
Section: Review Article
Country: India
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Sesbania grandiflora (Linn) belonging to family Leguminosae contains plenty of sterols, saponins and tannins which are responsible for its various pharmacological properties. The various parts of the plant like roots, bark, leaves, flower and fruit are known to possess different pharmacological properties. The information published about chemical composition and biological activity of Sesbania grandiflora of different genera is reviewed here. The available data on the biological action of individual constituents is presented. The perspectives of research and therapeutic use of Sesbania grandiflora as an excipient, hydrophilic matrix material, natural polysaccharide, binding agent and as a drug in ophthalmic dosage form are discussed here in terms of phytopharmaceutics.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000525
Title: Development of validated HPTLC method for quantification of Jatamansone in Jatamansi oil
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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In the present study an attempt has been made to develop a new, simple, sensitive, precise and robust high-performance thin layer chromatographic (HPTLC) method for the quantitative estimation of jatamansone in a steam distilled extract of rhizomes of Nardostachys jatamansi (Family: Valerianaceae). Analysis of jatamansone was performed on TLC aluminium plates pre-coated with silica gel 60F-254 as stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of petroleum ether-ether (3:1, v/v) at room temperature (25±2oC). Camag TLC scanner III was used for spectrodensitometric scanning and analysis of the plate in absorbance mode at 285 nm. The system was found to give compact spots for jatamansone (Rf value of 0.43+ 0.04). The data for calibration plots showed good linear relationship with r2 = 0.9980 in the concentration range of 250-1500ng/spot with respect to peak area. Jatamansone was found to be 20.32% in jatamansi oil. The present method was validated for precision and recovery. The limits of detection and quantification were determined. Statistical analysis of the data showed that the method is reproducible and selective for estimation of jatamansone.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000524
Title: Magnetic microsphere: as targeted drug delivery
Category: Pharmaceutics
Section: Review Article
Country: India
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Magnetic microspheres as an alternative to traditional radiation methods which use highly penetrating radiation that is absorbed throughout the body. Its use is limited by toxicity and side effects. The aim of the specific targeting is to enhance the efficiency of drug delivery & at the same time to reduce the toxicity & side effects. The present paper reviews the mechanism, preparation and applications of magnetic microspheres.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000522
Title: Evaluation of ethanolic extract of root of Bergenia ligulata for hepatoprotective, diuretic and antipyretic activities
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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The Ethanolic extract of the roots of Bergenia ligulata were assessed for Hepatoprotective, Diuretic and Antipyretic activities in albino rats that was compared with standard drugs. Evaluation of the Hepatoprotective activity was done by measuring the levels of Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Alkaline Phosphatase (ALP) and Total Bilirubin levels. Evaluation of the Diuretic activity was done by measuring the volume of urine collected at the end of 5 hrs and Na+, K+ and Clconcentrations in the urine. Rectal temperature was recorded at a time interval of 0, 30 min, 1h, 2h, 3h after drug administration for evaluation of Antipyretic activity. The Ethanolic extract of the roots of Bergenia ligulata was found to produce significant activities.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000520
Title: Development and characterization of Stavudine microspheres prepared using different polymers.
Category: Pharmaceutics
Section: Research Article
Country: India
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The objective of present study was to encapsulate the anti retroviral drug in various polymers in order to provide the sustained release and to minimize or reduce the dose dependent side effects as well as to improve patient compliance. Stavudine microspheres were prepared by solvent evaporation method using three different polymers viz. ethylcellulose, cellulose acetate and Eudragit S100. The prepared microspheres were evaluated for encapsulation efficiency, particle size analysis, surface morphology and in-vitro drug release study. The in-vitro drug release was performed in both simulated gastric and simulated intestinal fluid. The resultant microspheres were discrete and free flowing. Mean particle size of microspheres was in range of 110-145 μm for different formulations. The encapsulation efficiency was in range of 37-56% and found to be dependent upon the polymer with highest encapsulation was obtained in case of cellulose acetate. The FTIR and DSC confirmed the stable character of stavudine in drug loaded microspheres. The in-vitro drug release data was fitted into various kinetic models in order to find out the release mechanism.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000518
Title: Formulation and evaluation of mucoadhesive beads of glipizide using 23 factorial design
Category: Pharmaceutics
Section: Research Article
Country: India
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Glipizide is a second-generation sulfonylurea that can acutely lower the blood glucose level in humans and is typically prescribed to treat type II Diabetes [Non–Insulin Dependent Diabetes Mellitus]. Its short biological half-life [3.4±0.7 hours] necessitates that it be administered in two or three doses of 2.5 to 10 mg per day and the problem associated with gastrointestinal disturbances, attempts have been made to develop sustained release product. In the present investigation mucoadhesive beads of Glipizide were formulated employing sodium alginate, carbapol 974P and SCMC. The prepared formulations were evaluated for physical parameter, mucoadhesive properties, in vitro wash-off test and in vivo test by Folin-wv method. In-vitro drug release study was performed using USP XXI Basket apparatus and examined in simulated gastric fluid (Buffer pH 1.2 from 0 to 2 hours) and intestinal fluid (Buffer pH 7.4 from 2 to 8 hours). From Yates Algorithm it has been found that the carbapol 974P polymer is having more mucoadhesive property.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000516
Title: Antifertility activity of ethanolic extract and its n-hexane and benzene fractions of Heliotropium indicum leaves on Albino rats
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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With the folkrolic support, Heliotropium indicum (Boraginaceae) was identified, collected and authenticated. The leaves of H. indicum plant are separated, shade dried and extracted with ethanol then fractionated with n-hexane and benzene. Acute toxicity studies carried out according to OECD guidelines no.423 and oral doses of 200 and 400 mg/kg body weight are fixed. Albino rats are used for the study. Ethanolic extract and its fractions are subjected for contraceptive studies using the models like Anti-implantation, Abortifacient. Sperm motility an invitro study was carried out using 1%,2.5%,5% and 10% concentrations of extract and its fractions at various time intervals like 0,15,30,60,90,120 and 150 seconds respectively. Present study has shown better anti implantation activity with n-hexane rather than benzene fraction and ethanolic extract. In case of abortifacient model, higher dose of 400mg/kg body weight shown better activity. Similarly in sperm motility an invitro study ,better activity observed at 10% concentration.Study reveals that plant has better abortifacient activity and moderate antiimplantation and sperm motility.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000517
Title: Development of dissolution medium for carvedilol tablets
Category: Pharmaceutics
Section: Research Article
Country: India
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Carvedilol is a non-selective beta blocker indicated in the treatment of mild to moderate congestive heart failure, and it is listed in class 2 of biopharmaceutic classification of drugs. Carvedilol is a poorly water-soluble and highly permeable drug. In the present study a new dissolution medium was developed, as there is no official dissolution medium available in the literature. The composition of the dissolution medium was selected on the basis of solubility data at 37°C. Purified water and 0.1 N HCl containing 1% SLS showed increase in solubility and satisfying sink condition. The discriminating power of the selected two different dissolution medium (1 % sodium lauryl sulphate in water and in 0.1 N HCL) was evaluated for two commercial Carvedilol tablets. The rate of dissolution for commercial formulations was high in purified water compared to HCl containing SLS. It is concluded that 1% sodium laural sulphate in water is suitable dissolution medium for carvedilol.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000515
Title: Solubility Enhancement and Evaluation of Diacerein Using Cyclodextrin as Hydrophilic Carriers
Category: Pharmaceutics
Section: Research Article
Country: India
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Cyclodextrins, hydrophilic carriers, are cyclic polysaccharides which have property of forming inclusion complex with various guest molecules with suitable polarity and dimensions. They have been used extensively for solubility enhancement of hydrophobic drug/waterinsoluble drugs. Diacerein is an oral agent described as 4,5-Bis (acetyloxy)-9,10,dioxo-2-anthracenecarboxyllic acid. Diacerein is anthraquinone compound designed to treatment of osteoarthritis. The purpose of this research was to enhance the solubility and therefore dissolution of Diacerein by complexation with ß-cyclodextrin (ßCD) and hydroxyl propyl ß-cyclodextrin (HP ßCD).This study represents the formulation of inclusion complex with ßCD and HP ß CD by kneading method and their characterization and evaluation of improvement in dissolution profile. Phase solubility studies were performed according to method reported by Higuchi and Conners. Phase solubility studies indicated complex with possible stoichiometry of 1:1 Diacerein and for both ßCD and HPßCD. The inclusion complexes were characterized by FTIR, X ray diffraction and differential scanning colorimetry studies. The characterization confirmed inclusion of ring of Diacerein within nonpolar cavity of ßCD and HPßCD. When compared to pure drug and physical mixtures, remarkable enhancement in invitro drug release profile in PH 6.8 phsphate buffer was obtained in both inclusion complexes of ßCD and HP ßCD, especially with HP ßCD. Complexation of Diacerein with hydrophilic carriers like ßCD and HP ßCD offers a major enhancement in drug solubility.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000514
Title: Isolation and identification of anethole from Pimpinella anisum L. fruit oil. An antimicrobial study
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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The present study was conducted to isolate the most important bioactive compound from Pimpinella anisum L. fruit oil. The plant essential oil was extracted via steam distillation. Anethole was separated using a separatory funnel and detected on thin layer chromatography TLC plates in comparison with standard anethole that, served as positive control. Moreover, infrared FTIR spectrometer and High Performance Liquid Chromatography HPLC analysis were used to confirm the purity and identification of anethole. The isolated material was investigated for its antimicrobial activity against seven selected pathogenic microorganisms. The Gram-positive bacteria were Staphylococcus aureus, Bacillus cereus; and Gram-negative bacteria included; Escherichia coli, Proteus mirabils, Klebsiella pneumoniae, Pseudomonas aeruginosa; and the yeast Candida albicans. Anethole at different concentrations (1:1, 1:5, 1:10, 1:20) was active against all tested bacteria except for P. aeruginosa, and the highest inhibitory effect was observed against B. cereus (zone of inhibition: 22.3 mm) using the disc diffusion method. The minimal inhibitory concentration MIC of anethole was determined using a broth microdilution method in 96 multi-well microtiter plates. MIC values ranged from 31.2–500.0 μg/ml, and the most promising results were observed against S. aureus, B. cereus and E. coli (MIC 31.2 μg/ml) while, P. mirabilis and K. pneumonia ranked next (MIC 62.5 μg/ml). Furthermore, anethole achieved considerable antifungal activity against the yeast C. albicans (zone of inhibition range: 10.6–17.2 mm; MIC: 500.0).

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000512
Title: Bioadhesive-floating matrix tablet of salbutamol sulphate using response surface methodology: optimization and in vitro evaluation
Category: Pharmaceutics
Section: Research Article
Country: India
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The purpose of this research was to develop and optimize Bioadhesive-Floating Drug Delivery system (BFDDS) for salbutamol sulphate, which is absorbed from the upper part of GIT. A 23 full factorial design was applied to systematically optimize the drug release profile and bioadhesive force, with total polymer-to-drug ratio (X1), polymer-to-polymer (X2) and polymer grade (X3) as independent variables. The percentage drug release after 8 h, time required for 50 % drug dissolution (t50%) and bioadhesive force (f) were kept as dependent variables. Hydroxypropyl methyl cellulose (HPMC) of different viscosity grades and Carbopol 940P (CP940) were used in formulating the gastroretentive bioadhesive-floating drug delivery system. Response surface plots were drawn, and optimum formulations were selected by grid search method. Regression analysis and numerical optimization were preformed to identify the best formulation. It was found that HPMC, Carbopol and their interaction had significant impact on the release and bioadhesive properties of the delivery system. The decrease in the release rate was observed with an increase in the viscosity of the polymeric system but at higher concentration HPMC; different grades of HPMC (K4M and K100M CR) did not significantly affect the release profile. Kinetic treatment of dissolution profiles revealed that drug release ranges from anomalous transport to super case (II) type. The observed difference in the drug release and the bioadhesive properties of BFDDS could be attributed to the difference in the basic properties of three polymers (HPMC K4M, K100M CR and CP940) due to their water uptake potential and functional group substitution. The predicted values agreed well with the experimental values, and the results demonstrate the feasibility of the model in the development of bioadhesive-floating drug delivery system.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000501
Title: In vitro antioxidant and anticholinesterase activity of Acorus calamus and Nardostachys jatamansi rhizomes
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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The present study evaluated the free radical scavenging and anticholinesterase activity of the methanolic extracts of Acorus calamus (ACME) and Nardostachys jatamansi (NJME) rhizomes in vitro. In addition, total phenolics (TP) were also estimated. NJME contained significantly higher (p =0.05) phenolics (37 μg GAE/mg) than ACME (23μg GAE/mg). Consequently, NJME exhibited significantly higher (p =0.05) radical scavenging activity than ACME and BHT, a synthetic antioxidant. Further, the IC50 values were 704, 237 & 335 μg/ml for ACME, NJME and BHT respectively. In case of anticholinesterase activity also NJME exhibited significantly higher (p =0.05) activity with lower IC50 value than ACME. However, the anticholinesterase activity of both ACME and NJME were significantly lower (p =0.05) than neostigmine, a standard drug wherein, neostigmine exhibited significantly lower (p =0.05) IC50 value than ACME and NJME. Furthermore, a significant correlation between the total phenolic content, antioxidant and anticholinesterase activities of both the extracts indicating that total phenolics might be responsible for the observed antioxidant and anticholinesterase activities.This is the first report in this direction.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-P'Col-0000500
Title: Neuropharmacological evaluation of ethanolic extract of leaves of Bryophyllum pinnatum.
Category: Pharmacology
Section: Research Article
Country: India
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Neurpharmacological effects of ethanolic leaf extract of Bryophyllum pinnatum studied using animal models. The extract found to produce a profound decrease in exploratory activity in a dose-dependent manner. It also showed a marked sedative effect as evidenced by a significant reduction in gross behaviour and potentiation of pentobarbitone-induced sleeping time. Extract produced minor anticonvulsant effect by delaying seizure produced by strychnine and picrotoxin. The totality of these effects showed that the extract possesses depressant action on the central nervous system.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000499
Title: Evaluation of Antinociceptive Activity of Roots of Glycyrrhiza Glabra Linn.
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Present work was done to investigate the antiinflammatory and antinociceptive activity of aqueous and ethanolic extracts of Glycyrrhiza glabra by using different pain models in swiss albino mice of either sex. Antinociceptive activity was evaluated at 50-200mg/kg i.p. in mice using various experimentally induced pain models like Acetic acid induced abdominal constrictions, Formalin induced hyperalgesia and Tail flick method. Aqueous and ethanolic extract of Glycyrrhiza glabra inhibited Acetic acid induced abdominal constriction, with maximal effect 57.33 and 57.53% inhibition respectively at 200 mg/kg. In Formalin induced paw licking model, both extract exhibited more pronounced antinociceptive effect in inflammatory phase than in neurogenic phase (maximal effect were 43.24% and 40.93% for aqueous and ethanolic extract, respectively, at 200 mg/kg i.p). A dose dependant response was observed in Tail flick method but statistically significant maximal response was seen in mice at the dose of 200 mg/kg i.p. From the results it was concluded that both extracts exhibited anti-nociceptive activity by central and peripheral mechanism.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000498
Title: Quantification of Harmaline content in Passiflora foetida by HPTLC technique
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Passiflora foetida is commonly known as Stinking passion flower belongs to the family Passifloraceae. The aim of the study is to estimate one of the chemical constituents harmaline by High Performance Thin Layer Chromatagraphic technique using precoated silica F 254 Plates (Merck). The amount of harmiline was estimated by comparing the peak area of standard and that present in the extract. The harmaline content present in the extract was estimated to be 0.75% w/w.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-P'Col-0000527
Title: Antilithiatic effect of Achyranthus Aspera Linn. leaves extract on ethylene glycol induced nephrolithiasis
Category: Pharmacology
Section: Research Article
Country: India
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The ethanolic extract of leaves of “ Achyranthus aspera linn”. was screened for antilithiatic activity in male albino wister rats and result were summarized based on ionic changes in urine. Lithiasis (stone formation) induced by oral administration of 0.75% ethylene glycolated water to adult albino Wister rats for 28 days . The ionic chemistry of urine was altered by ethylene glycol , which elevates the urinary concentration of ions .eg. calcium , oxalate , phosphate , thereby contributing renal stone formation . Supplementation with ethanolic extract of leaves of. “ Achyranthus aspera linn”. Significantly reduce the elevated urinary calcium and oxalate ion concentration in the urine, confirming the stone inhibitory effect . Also it elevates the urinary concentration of magnesium, which is considered as one of the inhibitor of crystallation . The high urine creatinine level observed in ethylene glycol treated rats was also reduced following treatment with extract . The increased deposition of stone forming constituents in the kidneys of calculogenic rats was also significantly lowered by curative and preventive treatment using ethanolic extract . The histopathological findings also showed signs of improvement after treatment with ethanolic extract . all the observations provided the basis for the conclusion that the ethanolic extract of leaves of “ Achyranthus aspera linn”. is endonned with antilithiatic activity.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000526
Title: Preformulation and thermodynamic study of rizatriptan benzoate nasal gel formulation
Category: Pharmaceutics
Section: Research Article
Country: India
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Preformulation study was aimed for designing Rizatriptan benzoate nasal gel using Pluronic PF-127 to achieve improvement in bioavailability and patient compliance. In the present work, aqueous PF-127 gels (20-24 % w/w) containing drug, Sorbitol, PEG-6000, and sodium chloride were prepared by cold method. Effect of drug and gel additive on thermodynamic properties of phase transition at gelation (T1), gel-melting (T2) is reported. Gelation temperature (T1) decreased with increase in Pluronic concentration while gel-melting temperature (T2) increased. The Linearity was observed only for semi logarithmic plot of PF-concentration and 1/T2 for Sorbitol and PEG, which shows significant interaction of both at gel melting. Enthalpy of both transitions remains unchanged with drug indicating no interaction of drug with polymer.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000502
Title: Evaluation of Seed Flour of Vigna mungo (L.) based Sustained Release Matrix Tablets of Diclofenac Sodium
Category: Pharmaceutics
Section: Research Article
Country: India
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This study concerns the evaluation of seed flour of Vigna mungo or black gram as sustained release matrix forming material in tablet formulation. The physicochemical properties of seed flour of black gram such as swelling index, hydration capacity, powder porosity, loss on drying, pH and viscosity were studied. Matrix tablets were prepared by wet granulation technique using isopropyl alcohol as a granulating agent. Diclofenac sodium was used as a model drug. Sustained release matrix tablets of diclofenac sodium, were developed by using different drug: polymer ratios, such as BF1 (1:1), BF2 (1:2), BF3 (1:3) and BF4 (1:4). All the lubricated formulations were compressed using flat faced punches. Compressed tablets were evaluated for uniformity of weight, content of active ingredient, friability, hardness, thickness, in vitro dissolution using paddle method, and swelling behavior. All the formulations showed compliance with pharmacopoeial standards. Among different formulations, F1 showed sustained release of drug for 12 hours with 92.44% release. Tablets prepared with Hydroxypropylmethylcellulose (HPMC 50cps) and Xanthan gum as matrix forming material for the comparative study. The dissolution profiles of the HF2 and XF1 matrix tablets were close to the profile obtained by seed flour of black gram based matrix tablets BF1. The selected formulation (BF1) was subjected to stability studies for three months at 45° temperature with RH 75±5%, and showed stability with respect to release pattern. The kinetic treatment showed that the release of drug follows zero order model and anomalous diffusion for BF1 and XF1 while the drug release of HF2 was best explained by Higuchi’s model and anomalous diffusion. It is concluded that seed flour of black gram possess substantial matrix forming property that could be used for sustained drug delivery.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-P'Col-0000504
Title: Diuretic activity of Eupatorium odoratum Linn.
Category: Pharmacology
Section: Research Article
Country: India
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The diuretic activity for the infusions of Eupatorium odoratum Linn at 10, 20 and 30 % was evaluated in albino rats. Urinary excretion of water, pH, density, conductivity and Na +, K + and Cl - content were investigated in saline-loaded rats. The extract showed a dose-dependent decrease diuretic effect, but augmented significantly with respect to the control group for the urinary excretion of water and sodium. Furthermore, a potassium-sparing effect at 10 and 20 % was showed. The diuretic effect does not seem to be related to the potassium content of the starting material. The results justify the use of E.odoratum as diuretic agent by the Malaysian traditional medicine.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PHDRS-0000505
Title: Pharmacognostical studies on the Leaves of Alstonia scholaris R. Br.
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Alstonia scholaris R. Br. (Apocynaceae) is popularly known as “Saptaparni” or “Devil’s tree”, which is used in the traditional systems of medicine. The leaf is considered one of the important plant organs for the treatment of various disorders such as Beriberi disease, congestion of liver, in ulcer, dropsy, and stomachic pains. The current study has therefore been carried out to provide requisite pharmacognostic details. Morphological and anatomical aspects as well as differential microchemical response have been worked out to identify the diagnostic features of the leaf. Some of the diagnostic characters noted are verticillate arrangement of the leaves at node, dark green color of the upper surface, coriaceous nature, papillose abaxial epidermis and giant stomata. Physical constant values involving moisture content, ash and extractives as well as qualitative and quantitative estimation of various phytochemicals have been studied. The presence of lipid, saponin, tannin, alkaloid, phenol, steroid, flavonoid, and some other chemical constituents are recorded.

Journal: Journal of Pharmacy Research , Volume: 2, Issue: May
Article Id: JPRS-PCS-0000506
Title: Studies on colon targeted drug delivery system for tinidazole in the treatment of amoebiasis
Category: Pharmaceutics
Section: Research Article
Country: India
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Matrix tablet containing various proportion of guar gum were prepared by wet granulation technique using starch as a binder. All the formulation were evaluated for hardness, drug content uniformity, stability study, and were subjected to in-vitro drug release studies. The amount of tinidazole released from the matrix tablet at different time interval was estimated by UV method.Colon targeted matrix tablet of tinidazole containing 40% guar gum released 7% of tinidazole in the physiological environment of stomach ( 0.1N HCl) & small intestine (phosphate buffer 7.4 pH) & 91 % in the physiological environment of colon(phosphate buffer 6.8 pH). When the dissolution study was continued in simulated colonic fluids (rat caecal content medium) the matrix tablet containing 40% guar gum released another 98% of tinidazole after degradation into 2-3 pieces at the end of 24 h study.The result of the studies showed that colon targeted matrix tablet containing 40% of guar gum was most likely to provide targeting of tinidazole for local action in the colon. The colon targeted matrix tablet of tinidazole showed no change either in physical appearance, drug content or in dissolution pattern after storage at 400C / 75% RH for 2 month. IR spectrum showed no interaction between tinidazole & guar gum.