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Journal of Pharmacy Research
ISSN NO: 0974-6943
The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified when, we/or author get comments from any readers. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry,  Nursing, Paramedical, prescription etc  fields).
Scopus Indexed (link http://www.scimagojr.com/journalsearch.php?q=21100325431&tip=sid&clean=0) 

Journal Metrics for this Journal of Pharmacy Research (Source ID: 21100325431): 2015(SNIP) Source Normalized Impact Per Paper : 0.575; SCImago Journal Rank (SJR):0.787; Impact Per Publication : 0.789 CiteScore 2016: 0.93(Top level : Life Science)

Year SJR Cites per document Year Value
2014 0.607 Cites / Doc. (4 years) 2014 0.607
2015 0.787 Cites / Doc. (4 years) 2015 0.789
2016 0.926 Cites / Doc. (4 years) 2016 0.926
    Cites / Doc. (3 years) 2014 0.607
    Cites / Doc. (3 years) 2015 0.789
    Cites / Doc. (2 years) 2014 0.607
    Cites / Doc. (2 years) 2015 0.789
2016   Cites / Doc. (4 years) 2016 0.926
Cites Year Value
External Cites per document 2014 0.607
External Cites per document 2015 0.789
External Cites per document 2016 0.926
Cites per document 2014 0.607
Cites per document 2015 0.789
Cites per document 2016 0.926

Manuscripts Published

Journal: Journal of Pharmacy Research , Volume: 7, Issue: 1.
Article Id: JPRS-PC-00001321
Title: Evaluation of in vitro anthelmintic activities of novel 1,2,3 - benzotriazole derivatives synthesized in ultrasonic and solvent free conditions
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Objectives: To determine anthelmintic activity of the synthesized novel 1,2,3- benzotriazole derivatives under ultrasonication in solvent free conditions. Methods: Newer“1-(1Hebenzo[d][1,2,3]triazole-1-carbonyl) derivatives” (5Ae5P) were synthesizedby using“1Hebenzo[d][1,2,3]triazole”(1)as the startingmaterialunder ultrasonicated and solvent -free conditions. The resulting products were isolated and characterized by melting points and spectral studies. All the products were assayed for anthelmintic activity against Pheretima posthuma using albendazole and mebendazole as reference compounds. Results: All the newer 1,2,3 e benzotriazole derivatives synthesized by ultrasound activation in solventefree condition were obtained in moderate to good yields in the range of 71e82%. The data interpretation of the spectral values with reference to standard values confirmed the structures of the synthesized compounds. Out of the sixteen synthesized derivatives, four compounds (5B, 5F, 5J and 5N) showed anthelmintic activity in dose-dependent manner giving shortest time of paralysis and death with different concentrations of the derivatives. Among these four derivatives, 5J showed superior activity. Conclusion: Out of the sixteen synthesized derivatives, four compounds (5B, 5F, 5J and 5N) containing p-nitrophenyl substituent attached to azo group of benzotriazole moieties exhibited equal or comparable anthelmintic activity with reference to albendazole. The superior activity of compound 5J might be due to attachment of additional p-nitrophenyl substituent to the cyano group.

Journal: Journal of Pharmacy Research , Volume: 7, Issue: 1.
Article Id: JPRS-PA-00001320
Title: Application of a novel UPLC-MS/MS method for the pharmacokinetic/bioequivalence determination of atorvastatin and ezetimibe in human plasma
Category: Pharmaceutical Analysis
Section: Research Article
Country: India
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Aims: The aim of this study is to determine the pharmacokinetic parameters of atorvastatin calcium (AT) and ezetimibe (EZ) in human volunteers following the administration of a single oral dose of two drug products containing both active ingredients. Materials and methods: A novel, sensitive and selective ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of AT and EZ in human plasma, following administration of a single dose comprising AT 40 mg and EZ 10 mg, using a non-blind, twotreatment, two-period, randomized, crossover design, in twenty four healthy human volunteers. Atorvastatin, ezetimibe and the internal standard etilefrine (IS) were extracted from plasma and analyzed on a reversed-phase C18 column under gradient conditions. Results: The ion transitions monitored in multiple reaction-monitoring mode were 440.4, 271.25, 164.02 m/z derived from 559.57, 408.43, 182.12 m/z for AT, EZ and IS respectively. Calibration curves were generated over the range of 0.1e20 ng mL1 for both drugs with values for coefficient of correlation greater than 0.999. The parametric point estimates and the 90% confidence intervals for ln-transformed AUC0et, AUC0eN, and cmax, were within commonly accepted bioequivalence range of 80e125% range, thus the results reveal that the bioequivalence between the two drug products could be concluded. Conclusion: The method is very simple and allows obtaining a very good recovery of the analytes. The validated UPLCeMS/MS method has been applied to a pharmacokinetic/ bioequivalence study.

Journal: Journal of Pharmacy Research , Volume: 7, Issue: 1.
Article Id: JPRS-PB-00001308
Title: Phytochemical composition changes in untreated stem juice of Tinospora cordifolia (W) Mier during refrigerated storage
Category: Pharmaceutical Biotechnology
Section: Research Article
Country: India
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Background: Juice of Tinospora cordifolia is well reported for its immuno-modulatory and adaptogenic properties. Importance of the plant is well understood as its stem juice was widely used during the Swine flu break in India as an immuno-modulator. On the contrary, there are no data available on the shelf-life of juice. Therefore, effects of cold storage on the phytochemical profiles of T. cordifolia stem juice were analyzed. Methods: Juices of mature stems of the T. cordifolia were prepared and stored at 0 C. The juice samples were analysed on 0, 15 and 30 days intervals using UPLC-QTOFMS. The juice samples were resolved on C-18 (4.6  250 mm, 1.8 mm) column at 40 C. The optimized method provided a good linear relation (r  0.9213 for all the internal standards), satisfactory precision (RSD values less than 2.1%) and good recovery (97.3-101.2%). Results and discussion: Initial analysis of MSn data showed presence of 14,101 molecular features in the samples. Variation in the molecular features analysed using principal component analysis and discrimination models showed 99% variation across the samples analysed on 0, 15 and 30 day. Jatrorrhizine, mangoflorine, menisperine, columbamine, berberine and tinosporoside along with other most abundant metabolites were degraded 70-80% over a period of 15 days and 99% in 30 days. High degradation rate of metabolites constituents was observed may be due to the enzymes present in the juice or oxidative reactions. Therefore, it is recommended to use the fresh juice of T. cordifolia and stability of its herbal formulation should be analysed carefully.

Journal: Journal of Pharmacy Research , Volume: 7, Issue: 1.
Article Id: JPRS-MB-00001322
Title: Disodium edetate as a conjugation inhibitor: A novel approach to curb growing conjugal transfer of vanA in gram-positive bacteria
Category: Microbiology
Section: Research Article
Country: India
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Objective: The aim of the present study was to identify the vanA gene among clinical isolates of vancomycin-resistant Staphylococcus aureus (VRSA). Thereafter, transfer of vanA gene through conjugation from vanA positive VRSA to a vancomycin-sensitive S. aureus was evaluated. Next, we examined the effect of various concentrations of chemicals including ethylenediaminetetraacetic acid (disodium edetate), ethylene glycol tetraacetic acid (EGTA) and boric acid on conjugation. Methods: A total of fourteen clinical isolates of VRSA were analyzed for the presence of vanA gene using previously reported primer by polymerase chain reaction (PCR). The vanA positive isolate of VRSA served as donor and vancomycin-sensitive S. aureus (vanA negative) served as recipient. Conjugation was carried out according to the broth mating method in the absence and presence of various concentrations of chemicals. Results: The vanA gene was detected in eight of the clinical isolates of VRSA. Findings of our study revealed that vanA gene was successfully transferred in-vitro from VRSA donor to vancomycin-sensitive recipient S. aureus by a broth making procedure suggesting possibility of horizontal gene transfer (HGT). Of the evaluated chemicals on conjugation, disodium edetate and EGTA found to be inhibiting the conjugal transfer of vanA gene from donor to recipient. Interestingly, it was observed that disodium edetate at a concentration of 10 mM and above strongly inhibited conjugal transfer of vanA gene from donor to recipient while EGTA inhibited the same at 120 mM. However, boric acid failed to prevent conjugal transfer of vanA gene from donor to recipient. Conclusion: Bacteria transfer antibiotic resistance from one gram-positive species of bacteria to other bacterial species and thus generating multi-drug resistant bacterial strains. From above study, it can be conclude that disodium edetate at 10 mM and above exhibited a potential effect on the inhibition of transfer of vancomycin resistant gene vanA from vancomycin-resistant S. aureus to vancomycin-sensitive S. aureus. Therefore, the inhibition of conjugation process by 10 mM EDTA can be potentially a novel approach to combat spreading of antibiotic resistant gene.

Journal: Journal of Pharmacy Research , Volume: 7, Issue: 1.
Article Id: JPRS-PC-00001328
Title: Synthesis of novel 2,4-bis(substituted phenoxy)-6- (phenylthio) pyrimidine analogs and their antimicrobial activities
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Background/aims: Pyrimidines have a long and distinguished history extending from the days of their discovery as important constituents of nucleic acids to their current use in medicinal chemistry. As a part of project devoted to the development of pyrimidine analogs as antimicrobial agents we have focused our attention on synthesis of C5/C6 substituted pyrimidine derivatives, specifically C6 substituted pyrimidine analogs which have emerged as potent molecule in recent years. Methods: 2,4-Bis(substituted phenoxy)-6-(phenylthio)pyrimidines were prepared in five steps starting from barbituric acid. Initial reaction of barbituric acid (1) with POCl3 in presence of N,N-dimethylaniline under reflux furnished 2,4,6-trichloropyrimidine (2), which on hydrolysis with aq. NaOH under reflux yielded 6-chlorouracil (3). 6-Chlorourail on treatment with thiophenol in dry pyridine generated 6-phenylthiouracil (4), chlorination of 4 using excess POCl3 under reflux afforded the key synthon 2,4-dichloro-6-(phenylthio) pyrimidine (5). Aromatic nucleophillic substitution reaction of 5 with oxygen nucleophiles like sodium phenoxides provided the desired targeted compounds 6aeg in 62e86% yield. Structural assignments of the synthesized compounds were based on their IR, 1H NMR, mass and analytical data. The antimicrobial evaluation of newly synthesized compounds was carried out by cupeplate method. Results: The investigation of antimicrobial screening reveals that the compounds 5, 6b, 6c and 6f showed good activity against fungal strains comparable to the standard drug Flucanazole. Remaining compounds exhibited moderate activity against bacterial and fungal strains compared to standard drug. Conclusion: We have developed a facile methodology which avoids the use of moisture sensitive reagents like organolithiums, diphenyl disulphide, etc. The C6 substituted pyrimidine analogs can be considered for further studies as potent antifungal agents.

Journal: Journal of Pharmacy Research , Volume: 7, Issue: 1.
Article Id: JPRS-PCS-00001406
Title: Generic industry’s perceptions of generic medicines policies and practices in Malaysia
Category: Pharmaceutics
Section: Research Article
Country: India
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Objectives: Post-patent entry of generic medicines has been shown to reduce overall drug expenditure and increase access to medicines. However, the implementation of progeneric policies and practices are needed to create incentives for generic medicines production by the generic industry. This study assesses the views of the Malaysian generic drug manufacturers on existing policies and generic demand-sides practices in Malaysia. Methods: Data was gathered by using a mail survey approach. The questionnaire was mailed to all the members (N ¼ 26) of the Malaysian Organization of Pharmaceutical Industries (MOPI) licensed to manufacture prescription medicines in Malaysia. Results: Usable response rate was 53.8% following four successive mailings. Majority of the respondents (64.3%) were dissatisfied with generic prescribing in Malaysia, while majority of the respondents (57.1%) were satisfied with generic dispensing. Fifty-percent of the respondentswere dissatisfied with generic public awareness and equal proportions (21.4%) were either very dissatisfied or unsure. A majority of the respondents (69.2%) were dissatisfied with generic medicines education and information to healthcare professionals in Malaysia. The relationship between respondents’ perceived level of generic public awareness and generic prescribing was positive and significant (rs =0.59, p ¼ 0.03). Government policies and regulationswere perceived to be fairly effective in promoting generic medicines in Malaysia by 42.9% and 35.7%of the respondents respectively.Apositive and significant relationshipwas observed between respondents’ scores on government policies and regulations (rs = 0.55, p ¼ 0.04). Conclusions: Overall, the generic industry perceived generic dispensing in Malaysia to be somewhat satisfactory. However, generic prescribing, generic public awareness and education of healthcare professionals on generics need to be enhanced to foster generic uptake in Malaysia. The generic industry expressed ambiguous perceptions on effectiveness of government policies and regulations in promoting generic medicines in Malaysia.

Journal: Journal of Pharmacy Research , Volume: 7, Issue: 1.
Article Id: JPRS-PCS-00001407
Title: RP-HPLC determination of vildagliptin in pure and in tablet formulation
Category: Pharmaceutics
Section: Research Article
Country: India
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Aim: The present work aims to a simple, rapid and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of vildagliptin in pure form and in tablet dosage form using Agilent XDB C18, 150  4.6 mm, 5 mm, column. Methods: The mobile phase consists of 0.1 M Phosphate buffer and acetonitrile in the ratio of 85:15% v/v, and was pumped at rate of 1.0 mL/min. The detection was carried out at 210 nm and the calibration curve was linear in the concentration range of 10e150 mg/mL. Results: The method was statistically validated for its linearity, precision, accuracy, stability, specificity, LOD and LOQ. Due to its simplicity, rapidness, high precision and accuracy, the proposed RP-HPLC method may be used for determining vildagliptin in pure form and in tablet formulation. Conclusion: The proposed method was found to be simple, precise, accurate and rapid for determination of vildagliptin from pure form and tablet dosage form.