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Journal of Pharmacy Research
ISSN NO: 0974-6943
The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified when, we/or author get comments from any readers. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry,  Nursing, Paramedical, prescription etc  fields).
Scopus Indexed (link http://www.scimagojr.com/journalsearch.php?q=21100325431&tip=sid&clean=0) 

Journal Metrics for this Journal of Pharmacy Research (Source ID: 21100325431): 2015(SNIP) Source Normalized Impact Per Paper : 0.575; SCImago Journal Rank (SJR):0.787; Impact Per Publication : 0.789 CiteScore 2016: 0.93(Top level : Life Science)

Year SJR Cites per document Year Value
2014 0.607 Cites / Doc. (4 years) 2014 0.607
2015 0.787 Cites / Doc. (4 years) 2015 0.789
2016 0.926 Cites / Doc. (4 years) 2016 0.926
    Cites / Doc. (3 years) 2014 0.607
    Cites / Doc. (3 years) 2015 0.789
    Cites / Doc. (2 years) 2014 0.607
    Cites / Doc. (2 years) 2015 0.789
2016   Cites / Doc. (4 years) 2016 0.926
Cites Year Value
External Cites per document 2014 0.607
External Cites per document 2015 0.789
External Cites per document 2016 0.926
Cites per document 2014 0.607
Cites per document 2015 0.789
Cites per document 2016 0.926

Manuscripts Published

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-BMB-00001550
Title: Aldose reductase inhibitors from the fruits of Physalis peruviana Linn.- An In silico Approach
Category: Biochemistry and Molecular Biology
Section: Research Article
Country: India
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Background: The fruits of Physalis  peruviana Linn. have been widely used in the traditional medicine for various ailments. Recently, we have reported that the oral administration of Physalis peruviana Linn. fruit extract improves insulin sensitivity and ameliorates hyperglycemia in high-fat diet low dose STZ-induced type 2 diabetic rats. Molecular docking, the technique primarily employed for predicting and analyzing the interactions between protein receptors and ligands, is now an integral aspect in drug discovery and development area. Hence, the present study was aimed to screen the identified phytoconstituents of Physalis peruviana L. fruit to identify the potent constituent attributing for its antidiabetic activity using insilico approach. Methods : HPLC analysis of the ethanolic extract of fruits was performed using Shimadzu HPLC system equipped with a diode array detector. Docking studies on the constituents were carried out using AutoDock 4.2 software against the aldose reductase receptor. Results: HPLC analysis indicated that the major flavonoids present in the fruit extract include kaempferol, myricetin, quercetin and rutin and their binding energy was found to be -12.89, -12.99,-13.31,  -14.84, respectively. The activity was comparable with fidarestat, a standard drug. Conclusion: The study indicates the presence of biologically active flavonoids in the fruit extract and their interactions with aldose reductase. The insilico data provide vital clues that can be used to design new molecules with improved activity for the successful treatment of diabetes mellitus.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-ND-00001551
Title: Effect of thermal processing on in vitro antioxidant potential of Capsicum (Capsicum annuum) of different ripening stages
Category: Natural Drugs
Section: Research Article
Country: India
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Background: Capsicum (Capsicum annuum) is a very important vegetable consumed in most parts of India, not only for they impart color or flavor in cuisines, but also for their pharmacological activities. The cooking processes are different in the different part of this country, and therefore, the present study was designed to analyze their in vitro antioxidant profile before and after thermal processing in water, i.e pressure cooking and microwave treatment, which resemble cooking processes. Methods: The assays performed included ABTS and DPPH radical decolorization assays, reducing power assay, determination of total polyphenols as well as monomeric anthocyanin contents and assay for ascorbic acid contents of the red, yellow and green coloured capsicum of different ripening stages. Results and Discussion: The study indicated that yellow capsicum was better among the three regarding antioxidant profile as well as polyphenolics and ascorbic acid contents. Monomeric anthocyanin content, however, was found to be least in this variant. Upon microwave treatment, red and yellow variants showed improvement in their antioxidant potentials. Pressure cooking showed deterioration in antioxidant potential in some assay protocols. Conclusion: The improvement of antioxidant profiles of red and yellow capsicum could well be correlated with increase in total polyphenolics contents upon thermal treatment, which indicated probable solublization of otherwise less soluble bioactives in water on thermal treatment. The study indicated a systematic approach to ascertain antioxidant quality of capsicum of different ripening stages before and after thermal treatment with water.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-PCS-00001553
Title: A new approach to investigate drug release from liquid crystalline monoolein dispersions
Category: Pharmaceutics
Section: Research Article
Country: India
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Background: Many methods have been described to investigate the in vitro drug release from liquid crystalline monoolein dispersions, which appear to be of limited suitability due to the use of aqueous release media. In order to better mimic the environment encountered by the drug after administration, multilamellar vesicles (MLV) were used as a lipophilic compartment to which the transfer of a drug model was measured.  Methods:The detection of the substances transferred from the monoolein dispersions into the lipophilic compartment was performed using a centrifugation technique. Monoolein dispersions from monoolein and poloxamer 407 were prepared and loaded with the lipophilic drug model porphyrin.  Results and discussion: The transfer of porphyrin from the donor monoolein vesicles to the acceptor MLV was faster than from monoolein cubic particles and the final amount transferred from monoolein vesicles was higher than from monoolein cubic particles. With both donors the transfer stopped at a level less than the theoretical value. This difference in the rate and amount transferred might be attributed to the sponge like structure of the cubic particles. In addition, the cubic bicontinuous phase has a unique structure where the monoolein bilayer (internal structure) is extending in three dimensions, with a high specific bilayer/water interfacial area. Conclusion: Monoolein dispersions containing cubic particles or vesicles can very successfully be used as a drug carrier and the centrifugation technique is a suitable technique to study the transfer of porphyrin from the donor monoolein dispersions to the lipophilic acceptor MLV.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-ND-00001554
Title: Antioxidant, anticancer activities and a novel alkaloid of the aerial parts of Ricinus communis
Category: Natural Drugs
Section: Research Article
Country: India
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Background: The 50 % ethanolic crude extracts of the Egyptian variety of the aerial parts of the Ricinus communis was biologically in vitro tested for its antioxidant and anticancer activities and chromatographically investigated for its phyto-constituents as well. Methods: Antioxidant activity was calculated using 2.2-diphenyl-1-picryl hydrazyl (DPPH) radicals where the half maximal scavenging capacity (SC50) values for each tested sample was estimated. While, human breast carcinoma cells MCF-7 was used to evaluate the cytotoxic effect of the samples under test using the Sulphorhodamine B assay (SRB). Chromatographic investigation and structure elucidation was achieved by extensive chromatographic (CC and PC) and spectroscopic methods; 1D- (1H, 13C) and 2D-NMR spectra (HSQC, HMBC) as well as ESI-MS analysis. Results: Twelve natural bioactive compounds were isolated, among which a novel pure natural compound was isolated and identified as 1-(ethoxymethyl)-4-methoxy-2-oxo-1,2-dihydropyridine-3-carbonitril. The extract exerted significant antioxidant effects (SC50 58.7 μg/ml), as well as showed promising cytotoxic effect (IC50 17.9 μg/ml). Moreover, some isolated compounds were biologically investigated whereby the novel compound gave the highest cytotoxic activity with IC50 14.6 μg/ml. Conclusion: This present investigation revealed that the active 50% ethanolic extract rich in bioactive constituents could be used in pharmaceutical aspects as antioxidant and anticancer agents after further studies.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-BMB-00001555
Title: A Study of Prevalence of Extended Spectrum β- Lactamase (ESBLs) in Community & Hospital samples
Category: Biochemistry and Molecular Biology
Section: Research Article
Country: India
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Background: The increasing trend of multidrug resistance in microbes is a very serious problem worldwide. Production of ESBL is one of the most important mechanisms of resistance found among most of the Enterobacteriaceae members. Extended spectrum β -lactamase (ESBLs) are plasmid mediated enzymes conferring broad resistance to penicillin, cephalosporin and monobactams. A study was undertaken to know the occurrence of ESBLs in bacteria from clinical samples and their susceptibilities to various generation of β -lactam drugs. Methods: Clinical samples were collected from various hospitals and private pathological laboratories of Rajkot city. Bacterial strains were identified according to standard microbiological investigations approved by CLSI guideline.  Result and discussion: Out of 275 clinical samples collected from various hospitals and laboratories, 156 samples have been shown significant growth and out of those 69 samples were found to be â-lactam resistant strains. Strains which were 2nd generation and onwards â-lactam resistant were selected for detection of ESBLs. Over all 57.97% resistance was found to be due to ESBLs. Conclusion: ESBL is increasingly prevalent in the hospitals as well as community. This causes a serious therapeutic challenge in the future.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-PC-00001557
Title: Homology modeling and docking studies between Lanosterol 14 α-demethylase of Candida albicans and benzimidazole derivaties.
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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  1. Background and aim: Candida albicans is one of the most important causes of life threatening fungal infections in immunocompromised patients. The currently available antifungal drugs suffer from toxicity, greatest potential drug interactions with other drugs, insufficient pharmacokinetics properties, and development of resistance. Thus, development of new antifungal agents with optimum pharmacokinetics and less toxicity is urgent task. Lanosterol 14-α-demethylase is the target of azole antifungal agents. In the search for new azole antifungal, we have rationally designed benzimidazole derivatives as inhibitor of Lanosterol 14-α-demethylase of  Candida albicans. Method: This study describes building 3D model structure of cytochrome P450 Lanosterol 14-α-demethylase of  C.albicans (CACYP51) form Saccharomyces cerevisiae YJM 789 as template. The reliability of the models was assessed by Ramachandran plots and Profile-3D analysis. Molecular docking identified the binding mode of the benzimidazole derivatives with modeled CACYP51. Results and discussion: In the docking studies, we confirmed that all twelve compounds interact with the CACYP51, and imidazole (N-3) - heme coordination, hydrogen bonding, π- stacking and hydrophobic interactions. The derivatives (11e, 11k, 11l) with N-1 methyl, C2 asymmetric carbon and carbonylpiperazinyl side chain of benzimidazole were found to have significant interaction with active site of the receptor and docking score.Conclusion: Based on the results of this study, we conclude that the structural model of C. albicans can be used in benzimidazole derivatives optimization, virtual screening and de novo inhibitor design for the discovery of new antifungal agents.
Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-P'Col-00001558
Title: Comparative Analysis of Apoptotic Activity From Extracts of Saponin-rich Indian Medicinal Plants
Category: Pharmacology
Section: Research Article
Country: India
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Natural products have been regarded as an important source that could contribute to potential chemotherapeutic agents. In particular, plant derived compounds have gained importance in anticancer therapy. In our study, we investigated the inhibitory effects of different Indian medicinal plant extracts (Bacopa monerri, Tribulus terrestris, Asparagus racemosus and Nelumbo nucifera) on cancer cells which are known to be highly saponin producing. Saponins are a diverse group of compounds widely distributed in the plant kingdom, which are usually characterized by their structure containing a steroidal or triterpenoid aglycone and one or more sugar chains. In the present investigation, methanolic and ethanolic saponin rich plant extracts were compared for its cytotoxic effect on cancer and normal cell lines. The extracts also have been shown to induce apoptosis and cell cycle arrest in cancer cells which implies its anticancer efficacy.  Based on the current findings, it can be concluded that extracts from Nelumbia nucifera and Bacopa moneri have cytotoxic and apoptotic activity towards cancer cells which indicates the need of further investigation of these extracts for cancer therapeutics.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-PA-00001559
Title: Simultaneous equation method for the estimation of Atorvastatin calcium and Amlodipine besylate in bulk and in combined tablet dosage form using UV spectophotometry
Category: Pharmaceutical Analysis
Section: Research Article
Country: India
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A simple, accurate and precise method was developed for the simultaneous estimation of Atorvastatin Calcium (AVC), Amlodipine Besylate(AMB) and in their combined dosage form by UV Spectrophotometric Method. Both drugs Atorvastatin Calcium and Amlodipine Besylate are soluble in methanol. The wave length at which maximum absorption takes place for Atorvastatin Calcium and Amlodipine Besylate were found to be 240 nm and 234.9 nm respectively. Calibration curves were linear with correlation coefficient 0.998 and 0.999 over the concentration range of 5-30ìg/mL for Atorvastatin Calcium and 3-5ìg/mL for Amlodipine Besylate respectively. The developed methods were validated in terms of Accuracy, Precision, Limit of Detection and Limit of Quantification according to ICH guidelines. The LOD for AVC and AMB are 0.17ìg/mL and 0.05ìg/mL and the LOQ for AVC and AMB are 0.51ìg/mL and 0.165 ìg/mL. Precision for both the drugs were indicated by %RSD and found to be within the acceptance limits. Thus the method can be successfully applied in routine work for the determination of Atorvastatin and Amlodipine in combined dosage form.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-P'Col-00001560
Title: Aqueous bark extract of Terminalia arjuna protects against phenylhydrazine induced oxidative damage in goat red blood cell membrane protein, phospholipid asymmetry and structural morphology: a flow cy
Category: Pharmacology
Section: Research Article
Country: India
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Background: Phenylhydrazine(PHX) is one of the most investigated intracellular free radical generating probes which promote oxidative damage in erythrocytes and mimics the â thallasemia model. Terminalia arjuna (TA) bark, an indigenous plant used in ayurvedic medicine in India, primarily as a cardiotonic is also used in treating diabetes, anemia, tumors, hypertension,cancer,renal and hepatic disorder.TA has the hypocholesterolemic, hypolipidemic, antimutagenic, anti inflamatory, radioprotective and anti dysenteric activity. It is also used as anti coagulant and antioxidant. Objective: To evaluate the effect of aqueous bark extract of Terminalia arjuna (TA), against phenylhydrazine induced oxidative damage, in vitro, in goat red blood cell (RBC) membrane protein, phospholipid asymmetry and structural morphology. Methods: Oxidative stress biomarkers and activities of antioxidant enzymes were determined by biochemical analysis. RBC membrane protein damage was evaluated using SDS-PAGE followed by densitometric analysis. Phospholipid asymmetry of the RBC membrane was determined by thin layer chromatography followed by phosphate estimation. Iron concentration was estimated by using atomic absorption spectrophotometry (AAS). Morphological analysis of the RBCs were performed using scanning electron microscopy (SEM) and flow cytometry. Results: Aqueous bark extract of  TA protected the RBCs from phenylhydrazine (PHZ) induced oxidative damages in vitro as evident  from its effect on lipid peroxidation level, reduced glutathione and protein carbonyl content, and activities of antioxidant enzymes – superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx) and glutathione reductase(GR) .Membrane protein damage, phospholipid traslocation, and morphological alterations of RBCs induced by PHZ were also found to be protected   by aqueous bark extract of  TA. Conclusion: The present study indicated that the aqueous bark extract of  TA, protects against PHZ induced oxidative stress mediated damages in goat RBCs in vitro, possibly through its antioxidant activity and its  bioactive constituents may contribute toward such protection.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-BMB-00001561
Title: Therapeutic effect of Thymoquinone on 7, 12 dimethyl benz(a) anthracene (DMBA) induced experimental breast cancer
Category: Biochemistry and Molecular Biology
Section: Editorial
Country: India
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Breast cancer is one of the leading causes of morbidity and mortality among women. The incidence of breast cancer is lowest in less developed countries. The higher incidence is attributed to delayed first child birth, shorter duration of breast feeding, family history of breast cancer, early menarche, and late menopause. Westernization of lifestyle, changes in dietary habits, indiscriminate exposures to exogenous estrogens are often considered to be reasons for the higher incidence reported in developing countries. Bioactive principles of plant origin have been reported for their anticancer potential. Thymoquinone, the main active ingredient of the volatile oil isolated from the Nigella  sativa seeds has been shown to possess a wide array of pharmacological effects. In the absence of systemic studies in the literature, the present study was aimed to evaluate the chemotherapeutic potential of thymoquinone in 7, 12-dimethyl benz(a)anthracene (DMBA) induced mammary carcinoma in rats. Oral treatment of Thymoquinone (25 mg/kg BW) to breast tumor bearing rats daily for four weeks was found to be effective against DMBA induced mammary gland carcinogenesis in female Wistar rats. The increased levels of DNA and RNA were found to be decreased upon treatment with Thymoquinone. The increased activities of AST, ALT, ALP, ACP, 5’-ND, gamma-GT, LDH and AHH in serum of control and experimental breast cancer rats were significantly decreased to near normal levels. Also, the levels of enzymatic antioxidants such as SOD, CAT, GPx and non-enzymatic antioxidants such as GSH, Vitamin C, Vitamin E were improved upon treatment with thymoquinone.  Thus, the results of the present study clearly indicate that thymoquinone suppresses DMBA induced breast cancer in rats.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 12.
Article Id: JPRS-PHDRS-00001562
Title: Polyphenolic compounds and evaluation of antidiabetic activity of Ruprechtia salicifolia
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Objectives: This study explored the constitutive polyphenols isolated from leaves of Ruprechtia salicifolia (R. salicifolia) and evaluated its antidiabetic activity. Methods: Chromatographic separation for 80% MeOH leaf extract of R. Salicifolia was performed to isolate polyphenolic compounds, structures of the isolated compounds were established by different chromatographic and spectral techniques UV, MS, 1H and 13C NMR. Antidiabetic activity was evaluated through surcase enzyme inhibitory activity assay. Results: Six known phenolic compounds were isolated for the first time from this species including; querectin 3-O-α-L-glucuronopyranoside12,2,3-(S)-hexahydroxydiphenoyl-(α/β)Dglucopyranose 13, 1-O-galloyl-4,6-(S)-hexahydroxydiphenoyl-β-D-glucopyranose (strictinin) 14, 4-O-methyl-ellagic acid 15, kampferol 16 and ellagic acid 17. 80% MeOH leaf extract and the tested compounds showed a significant sucrase enzyme inhibitory activity. Conclusion: R. salicifolia contains phenolics of antidiabetic activity giving it a great potential as a medicinal natural drug.