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Journal of Pharmacy Research
ISSN NO: 0974-6943
The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified when, we/or author get comments from any readers. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry,  Nursing, Paramedical, prescription etc  fields).
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Journal Metrics for this Journal of Pharmacy Research (Source ID: 21100325431): 2015(SNIP) Source Normalized Impact Per Paper : 0.575; SCImago Journal Rank (SJR):0.787; Impact Per Publication : 0.789 CiteScore 2016: 0.93(Top level : Life Science)

Year SJR Cites per document Year Value
2014 0.607 Cites / Doc. (4 years) 2014 0.607
2015 0.787 Cites / Doc. (4 years) 2015 0.789
2016 0.926 Cites / Doc. (4 years) 2016 0.926
    Cites / Doc. (3 years) 2014 0.607
    Cites / Doc. (3 years) 2015 0.789
    Cites / Doc. (2 years) 2014 0.607
    Cites / Doc. (2 years) 2015 0.789
2016   Cites / Doc. (4 years) 2016 0.926
Cites Year Value
External Cites per document 2014 0.607
External Cites per document 2015 0.789
External Cites per document 2016 0.926
Cites per document 2014 0.607
Cites per document 2015 0.789
Cites per document 2016 0.926

Manuscripts Published

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-QA-0000208
Title: Derivative and ratio derivative spectroscopic methods for simultaneous determination of Cefalexin monohydrate and Kanamycin monosulphate using phenylisocyanate as derivatizing reagent
Category: Quality Assurance
Section: Research Article
Country: India
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Two simple, accurate, precise and economic spectrophotometric methods have been developed for simultaneous determination of Cefalexin monohydrate and Kanamycin monosulphate in their synthetic mixture by second order derivative and ratio derivative spectrophotometry. Kanamycin monosulphate lacks chromophore; and it cannot be detected by conventional detection system. Kanamycin monosulphate and Cefalexin Monohydrate both contain primary amine and were derivatized with Phenylisocyanate in presence of triethylamine at 70ºC for 10 minutes to yield corresponding stable N-aryl-N’-phenyl urea derivative. For second order derivative method, Zero cross point values at 250 and 242 nm were selected for the assay of Cefalexin Monohydrate and Kanamycin monosulphate, respectively. Calibration graphs were linear in the range 2-12 and 5-30 µg mL-1 for Cefalexin Monohydrate and Kanamycin monosulphate, respectively. For ratio derivative method, the peaks in the first order derivative of ratio spectra at 275 nm and 241 nm were selected to determine Cefalexin Monohydrate and Kanamycin monosulphate, respectively. Calibration graphs were linear in the range of 2-10 and 5-25 µg mL-1 for Cefalexin Monohydrate and Kanamycin monosulphate, respectively. The said methods were successfully applied for quantitative determination of both drugs in their synthetic binary mixtures. The proposed methods are simple, selective and suitable for the quality control application.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-PHDRS-0000207
Title: Evaluation of acute and subacute toxicity of aqueous extracts from Marrubium vulgare L in rodents
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Aim: The aim of this study was to investigate the safety of the aqueous extract of Marrubium vulgare by determining its pharmaco-toxicological effects after acute and sub-acute administration in mice and rats, respectively. Materials and methods: In acute study, a single administration of the aqueous extract of Marrubium vulgare was given orally to mice at doses ranging from 2 to 12 g/kg. General behaviour, adverse effects and mortality were determined for up to 14 days post treatment. In the subacute study, the extract was administered orally at doses of 0, 500, 1000, 2000 and 3000 mg/kg daily for 28 days to rats. Animal body weight was observed throughout the experimental period while hematological and biochemical parameters of blood as well as kidney and liver tissues histology were evaluated at the end of the experiment. Results: In the acute study in mice, none of the doses used induced mortality or significant behavioural changes. The sub-acute oral administration of the aqueous extract of Marrubium vulgare caused no noticeable change in the general behaviour of the rats, no significant changes in body weight, food intake and utilization of food in the treated rats. All hematological parameters measured remained within physiological range throughout the treatment period (28 days). Serum AST, ALT, ALP, glucose and triglycerides decreased significantly. Histological analysis did not show any liver or kidney alteration. Conclusion: it may be concluded that the aqueous extract of Marrubium vulgare does not appear to have significant toxicity. In view of the doses consumed empirically in traditional medicine in Morocco, there is a wide margin of safety for the therapeutic use of Marrubium vulgare.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-MB-0000206
Title: Macrolide resistance and biofilm formation in Streptococcus pyogenes infection
Category: Microbiology
Section: Research Article
Country: India
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Streptococcus pyogenes is a notorious human pathogen causing a wide range of infection from mild pharyngitis to life threatening infections. Biofilm formation of S. pyogenes is an innate property during the pathogenesis of invasive infections. Aim: This study is aimed to isolate Streptococcus pyogenes from respiratory tract infections and to evaluate the macrolide resistance and biofilm formation capacity among them. Materials and methods. A total of 71 Streptococccus pyogenes were isolated. Antibiotic sensitivity testing was done for all isolates; Double disk diffusion test was done for all macrolide resistant strains with Erythromycin and clindamycin. All isolates were screened for biofilm formation by Tissue culture plate method. Result: Out of 71 Streptococcus pyogenes isolated, all strains were completely sensitive to Penicillin, Amoxicillin, Cephalothin and cefuroxime but showed 28.1% resistance to Macrolides,11.26% to Clindamycin, 12.7% to Ofloxacin and 17% to Chloramphenicol. Among 71isolates,25 (35.2%) isolates were positive for biofilm formation by Tissue culture plate method. Conclusion: There is high rate of macrolide resistance among Streptococcus pyogenes isolate in this locality. Biofilm formations more prevalent among Macrolide sensitive strains, which is may be the reason for having chronic infection of Streptococcus pyogenes .

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-ORS-0000205
Title: Effect of amla extract on bacterial infection in the Silkworm, Bombyx mori L.
Category: Others ( Related Science )
Section: Research Article
Country: India
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During silkworm - Bombyx mori rearing, larvae frequently acquire bacterial infection leading to mortality. Growing infection needs to be controlled in order to increase the silk production. In a view, an induced flacherie by Bacillus subtilis infection was controlled by amla aqueous extract showing antibacterial action in both In vitro and In vivo experiments. Suggestively, 10% amla extract spraying in an intermittent period during rearing resulted in an improved defensive mechanism as evidenced from the study to improve silk productivity.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-PCS-0000204
Title: Formulation, Characterization and in vitro release of Proliposomes for topical delivery of Aceclofenac.
Category: Pharmaceutics
Section: Research Article
Country: India
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Background: Patients with rheumatic disease are usually prescribed with non-steroidal anti-inflammatory drugs which causes increased gastrointestinal complications. The aim of the current study was to formulate and characterized a vesicular type of drug carrier system such as proliposomal, system for transdermal delivery of aceclofenac in order to avoid the problems associated with oral administration. Methods: Aceclofenac proliposomes were prepared by Xiao et al., method by film-deposition. So prepared proliposomes were characterized for their size, surface morphology, drug content, percent encapsulation efficiency, drug retained in skin, in vitro permeation studies and stability studies. Drug-excipient interaction was studied by FT-IR. Results: The results revealed no interaction between drug and the excipients, SEM photography confirmed spherical shaped proliposomes of aceclofenac with an average size of about 35µm. The maximum drug content and percent entrapment efficiency were found to be 96.23±0.6, 79.5±1.92 respectively. Maximum drug permeation was seen in F4 formulation where the lecithin concentration was optimum. The prepared liposomes were found to be stable at lower temperatures. Conclusion: To conclude, aceclofenac loaded liposomes seem to be promising controlled delivery system.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-PA-0000203
Title: HPLC method development and its validation for the determination of Triflusal in pharmaceutical formulations
Category: Pharmaceutical Analysis
Section: Research Article
Country: India
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An HPLC method has been developed and validated for the determination of triflusal in bulk drug and pharmaceutical formulations which is simple, selective & sensitive. Triflusal is estimated from its formulation by HPLC on a Kromasil C18 column by using acetonitrile: orthophosphoric acid (40:60 v/v) as mobile phase at a flow rate of 1.0 ml/min with detection at 237 nm. The specificity, limit of detection, limit of quantification, linearity, accuracy, robustness and precision are found to be acceptable over the concentration ranges of 40-60ppm for triflusal. Triflusal was subjected to stress conditions of hydrolysis, oxidation and thermal degradation and was found to be unstable in all these stress conditions. The optimized methods proved to be specific, robust and accurate for the quality control of triflusal in bulk drug and its pharmaceutical formulations.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-P'Col-0000202
Title: Evaluation of estrogenic activity of methanolic extract of Mikania scandens
Category: Pharmacology
Section: Research Article
Country: India
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Objective: To evaluate estrogenic activity of methanolic extract of Mikania scandens aerial parts in female albino rats. Methods: The estrogenic activity was determined using vaginal cornification and uterine weight methods. Ovariectomised female rats were treated with methanolic extract at dose of 400mg/kg body weight alone as well as with ethinyl estradiol (0.02mg/kg body wt) continuously for seven days by oral administration. On the 8th day, all animals were sacrificed and uterine weight, vaginal cornification, diameter of uterus, thickness of endometrium were observed. Cholesterol and total protein were estimated by using Liebermann-Burchard and Folin-Lowry method respectively. Results: The methanolic extract exhibited significant (p< 0.05) estrogenic activity as evidenced by increase in the uterine weight, diameter of uterus, thickness of endometrium compared to control. A significant increase (p< 0.05) in uterine cholesterol and total protein level in treated rats was also observed. Conclusions: Methanolic extract possesses estrogenic effect which may be attributed to the presence of steroids like stigmasterol and â-sitosterol.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-MB-0000201
Title: A study of biofilm formation in multidrug resistant organisms.
Category: Microbiology
Section: Research Article
Country: India
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The most challenging problem in the management of bacterial infections is the multi-drug resistant organisms (MDRO) that are compounded by biofilm formation. The present study is designed to know the antibiotic resistance patterns of MDRO and their association with biofilm. Various clinical samples such as urine (n=48), pus (n=28), sputum (n=160 and blood (n=8) were processed by standard procedure for identification of isolates. Antibiotic susceptibility testing was done to know the resistance pattern. Biofilm production was identified by microtitre plate stained with 0.1% crystal violet and 0.1% safranine staining. 84 samples yielded growth (isolates n=86). 75 MDR isolates were identified of which 30 were MDR GPC and 45 were MDR GNB. 28 of the 75 MDRO were strong biofilm producer using 0.1% safranine staining. The present study shows a significant association of MDR isolates from pus with biofilm formation.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-PC-0000200
Title: Synthesis and evaluation of anti-inflammatory activity of some 1, 2, 3, 4- Tetrahydropyrimidines
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Twelve [4, 6 (4- substituted aryl)-2-oxo-1, 2, 3, 4-tetrahydropyrimidine-5-yl] propionic acid derivatives have been synthesized in a two-step reaction. In the first step benzene or substituted benzene react with glutaric anhydride in presence of aluminum trichloride (Friedel Craft reaction) to obtain 5-(substituted phenyl)-5-oxo pentanoic acid. Second step involves synthesis of [4, 6 (4-substituted aryl)-2-oxo-1, 2, 3, 4-tetrahydropyrimine-5 yl propionic acid by reaction between 5-(substituted phenyl)-5-oxo pentanoic acid, urea and substituted aldehydes (Biginelli reaction). Their structures are confirmed by IR, 1H NMR. T.L.C. of synthesized compounds performed in chloroform: ethanol (3:1) solvent system. The anti-inflammatory activity of all compounds has been recorded on the basis of reference standard Indomethacin. All the compounds showed tendency to cause a fall in oedema and showed anti-inflammatory activity. The anti-inflammatory data shows that use of anisole in first step plays important role in the activity. Anti-inflammatory activity of all compounds was taken by Carrageenan induced rat paw oedema as described by Winter et al. on albino rats.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-PCS-0000199
Title: Development and Validation For The Simultaneous Estimation of Sparfloxacin and Dexamethasone by Reverse Phase High Performance Liquid Chromatographic Method
Category: Pharmaceutics
Section: Research Article
Country: India
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In the present study an RP-HPLC method was developed and validated for the simultaneous determination of Sparfloxacin and Dexamethasone. The chromatographic system was equipped with SUPELCO 516 C18 column and UV detector set at 254 nm, in conjunction with a mobile phase of 0.05 M Potassium Dihydrogen Phosphate buffer and Acetonitrile in the ratio of 50:50 (pH 4, adjusted with orthophosphoric acid) at a flow rate of 1.0 ml/min and the injection volume set at 20 µl with 10 minutes of runtime. The described method was linear over a concentration range of 5-45 µg/ml for the simultaneous estimation of Sparfloxacin and Dexamethasone with a with good linearity response of 0.9996 and 0.9979 respectively. The retention time of Sparfloxacin was 4.127 min ± 0.16 and of Dexamethasone was 5.727 min ± 0.10. The results of analysis were validated. The results of the study showed that the proposed RP-HPLC method was simple, rapid, precise and accurate, which is useful for the routine determination of Sparfloxacin and Dexamethasone in bulk drug and in pharmaceutical dosage form.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-PCS-0000198
Title: Formulation and In Vivo Evaluation of Immediate Release Glimepiride Coated Pellets Using 32 Full Factorial design by Novel Liquid Layering Technology
Category: Pharmaceutics
Section: Research Article
Country: India
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The aim of the present study was to develop immediate release liquid coated pellets of glimepiride with hydroxy propyl cellulose-LF as hydrophilic polymer and PVP K30 as binder. In this study, a 32 full factorial design was employed to optimize the formulation of pellets. In order to optimize formulations, the amount of HPC LF and the amount of PVP K 30, were chosen as independent variables. The prepared pellets were evaluated for drug content, particle size, subjected to Scanning Electron Microscopy (SEM), FT-IR & Differential Scanning Calorimetry (DSC) and evaluated for in vitro release. The drug content was in the range of 98.78% to 99.72%. The mean particle size of the drug-loaded pellets was in the range 735-775 ìm. Rapid drug release was observed in F9 as 99.47 using HPC LF and PVP K 30 with 3% and 1% concentration. The optimized formulation (F9) shows highest percent of drug release 99.96 by the end of 8 min, when compared with the marketed product (DIAPRIDE 2 mg) i.e., 70.29 within 8 min. SEM photographs confirmed that the prepared formulations were spherical in nature with a smooth surface. The compatibility between drug and polymers in the drug-loaded pellets was confirmed by FT-IR & DSC studies. In vivo bioavailability studies indicated significance difference between Glimepiride coated pellets and pure drug. Therefore, the present Glimepiride coated pellets is considered to be potentially useful for the treatment where improved patient compliance and convenience is expected.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-NDDS-0000197
Title: Liposomes for controlled drug delivery: drugs of the future
Category: Novel Drug Delivery Systems
Section: Review Article
Country: India
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The objective of drug delivery systems is to deliver a drug effectively, specifically to the site of action, in a controlled manner and to achieve greater efficacy and minimize the toxic effects compared to conventional drugs. Liposomes are the leading drug delivery systems for the systemic (intravenous) administration of drugs. The real breakthrough developments in the area during the past 15 years have resulted in the approval of several liposomal drugs, and the appearance of many unique biomedical products and technologies involving liposomes. There are now liposomal formulations of conventional drugs that have received clinical approval and many others in clinical trials that bring benefits of reduced toxicity and enhanced efficacy for the treatment of cancer and other life-threatening diseases.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-PC-0000196
Title: Synthesis of novel Triazolo Thienopyrimidine derivatives as antimicrobial agents – A physicochemical study
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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In this study, a series of novel 2-Methyl-4-(1-methyl-1H-[1,2,3] triazol-4-yl-methoxy)-5,6,7,8-tetrahydra-benzo[4,5]thieno[2,3-d]pyrimidine derivatives (IVa-e) were synthesized by treating 2-Methyl/Phenyl-4-prop-2-ynyloxy-5,6,7,8-tetrahydro benzo[4,5] thieno [2,3-d] pyrimidine (IIIa/b) with alkyl azides in the presence of copper sulphate and dimethylsulphoxide. By treating 2-Methyl-5,6,7,8,-tetrahydrobenzo[b]thieno [2,3-d]pyrimidine-4-one(II) and its derivatives with propargyl bromide in the presence of potassium carbonate in acetone results in the formation of O-substituted compound-III and its derivatives. The compound-II and its derivatives were synthesized by treating 2-amino-thiophene -3-carboxylic acid ethyl ester (I) with alkyl/aryl nitrile in the presence of dry HCl and 1,4-dioxane. The newly synthesized compounds (IVa-e) were purified, separated, and characterized by TLC, IR, and Mass spectra. These representative analogues were screened for invitro antimicrobial activity. The compounds exhibited significant antibacterial and antifungal activities.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-MAP-0000194
Title: Phenolic Metaboloites and Antioxidant Activity of Codiaeum variegatum CV. spirale
Category: Medicinal and Aromatic Plants
Section: Research Article
Country: India
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The methanol extract of Codiaeum variegatum cv. spirale yielded five flavonoids; apigenin, orientin, vitexin, isovitexin and vicenin-2 along with phenolic acids known as caffeic and r-coumaric acids. Structures of the isolated compounds were determined by using chromatography, MS and 1H, 13C NMR spectroscopy. The antioxidant activity of the main isolated compounds; apigenin, vitexin and isovitexin was investigated using the DPPH, total reduction capability and inhibition of lipid peroxidation assay. Vitexin exhibited the highest DPPH radical scavenging antioxidant activity (IC50 = 8.95 μg ml-1). In addition, vitexin and isovitexin showed a significant reducing power (RP50 = 8.65 and 9.27 μg ml-1, respectively). While, apigenin was found to be the active compound against the lipid peroxidation process (IC50 = 31.82 μg ml-1).

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-MB-0000193
Title: Detection of CTX-M, CTX-M 15, TEM and SHV type Extended Spectrum Beta lactamase genes among Gram negative clinical strains isolated from Government hospital, Mangalore.
Category: Microbiology
Section: Research Article
Country: India
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Aim: This study was carried out to evaluate the existence of the CTX-M, CTX-M 15, TEM and SHV genes in ESBL producing strains of Gram negative clinical isolates from Government Hospital, Mangalore, Karnataka (India). Materials and Methods: A total of 75 isolates were collected including family Enterobacteriaceae, Acinetobacter spp and Pseudomonas spp.The isolates were phenotypically confirmed as ESBL producers which were further evaluated for CTX-M, CTX-M 15, TEM and SHV genes by multiplex PCR. Results: Of the 75 isolates, PCR assay revealed that 51(68%), 3(4%), 21(28%) and22 (29.3%) were positive for CTX-M, CTX-M 15, TEM and SHV genes respectively. Conclusion: Molecular identification of the genes encoding beta lactamases would be essential for a reliable epidemiological investigation of their transmission in hospitals and antimicrobial resistance.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 5.
Article Id: JPRS-ND-0000190
Title: Total phenolic content and free radical scavenging activity of Pterocarpus marsupium heartwood & Tribulus terrestris dry fruits: An in vitro comparative study
Category: Natural Drugs
Section: Research Article
Country: India
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Background: Majority of the disorders like diabetes, atherosclerosis, cancer and neurodegenerative diseases are linked to oxidative stress. Antioxidants derived from plant sources offer resistance against the oxidative stress by way of their free radicals scavenging & lipid peroxidation inhibitory actions. Present study was designed to compare the free radical scavenging activity in relation to the total phenolic content of P. marsupium heartwood & T. terrestris dry fruits. Methods: Total phenolic content of the extracts was determined by using Folin-Ciocalteu method following a slightly modified method of Singleton et al by comparing with standard Gallic acid. The ability to scavenge 1, 1-diphenyl-2-picryl-hydrazyl(DPPH) radical is measured by a decrease in the absorbance at 517 nm as described by Hou et al comparing with standard Ascorbic acid. Results: Phenolic contents ranging from 45 to 160 and 5 to 25mg of Gallic acid equivalents (GAE) per gram of extract were present in P. marsupium & T. terrestris respectively. At 100ìg concentration P. marsupium & T. terrestris produced 85.9 & 67.5% of DPPH scavenging activity respectively and the standard ascorbic acid(10ìg) produced 90% scavenging activity. Conclusion: The present study concludes that both the plant extracts have shown significant in vitro free radical scavenging activity. Among them, aqueous extract of P. marsupium heartwood exhibited better activity which can be attributed to the presence of high phenolic content. Hence these herbal constituents are of adjuvant importance in the management of various free-radical mediated pathological conditions.