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Journal of Pharmacy Research
ISSN NO: 0974-6943
The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified when, we/or author get comments from any readers. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry,  Nursing, Paramedical, prescription etc  fields).
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Journal Metrics for this Journal of Pharmacy Research (Source ID: 21100325431): 2015(SNIP) Source Normalized Impact Per Paper : 0.575; SCImago Journal Rank (SJR):0.787; Impact Per Publication : 0.789 CiteScore 2016: 0.93(Top level : Life Science)

Year SJR Cites per document Year Value
2014 0.607 Cites / Doc. (4 years) 2014 0.607
2015 0.787 Cites / Doc. (4 years) 2015 0.789
2016 0.926 Cites / Doc. (4 years) 2016 0.926
    Cites / Doc. (3 years) 2014 0.607
    Cites / Doc. (3 years) 2015 0.789
    Cites / Doc. (2 years) 2014 0.607
    Cites / Doc. (2 years) 2015 0.789
2016   Cites / Doc. (4 years) 2016 0.926
Cites Year Value
External Cites per document 2014 0.607
External Cites per document 2015 0.789
External Cites per document 2016 0.926
Cites per document 2014 0.607
Cites per document 2015 0.789
Cites per document 2016 0.926

Manuscripts Published

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-MPS-0000402
Title: Antioxidant mechanism(s) of protective effects of Fenugreek 4-hydroxyisoleucine and trigonelline enriched fraction (TF4H (28%)) Sugaheal® against copper-ascorbate induced injury to goat cardiac mit
Category: Mechanism / Pathway Study
Section: Research Article
Country: India
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Sugaheal®, the 4-hydroxyisoleucine and trigonelline enriched fraction (TF4H (28%)) isolated from the seed of Trigonella foenum graecum (Linn.) is a well established antidiabetic drug. But the mechanism of its protection against diabetes is still unknown. Our experiments has revealed that this fraction (TF4H (28%)) Sugaheal® also possesses good antioxidant characteristics which is evident from its capacity to scavenge hydroxyl radical, superoxide anion radical, hydrogen peroxide and DPPH radical in chemically defined in vitro system.TF4H (28%) Sugaheal® also prevented the increase in lipid peroxidation level, protein carbonyl level and a concomitant decrease in reduced glutathione level in goat heart mitochondria against Cu-ascorbate induced oxidative stress in an in vitro system. These above facts are also confirmed by its inhibition of increase in Mn-SOD activity and induction of glutathione reductase (the reduced glutathione synthesizing enzyme) and Krebs cycle enzyme activity, which makes its antioxidant phenomenon.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-MB-0000226
Title: In vivo antiplasmodial activity of four folklore medicinal plants used among tribal communities of Western Ghats, Coimbatore, Tamil Nadu
Category: Microbiology
Section: Research Article
Country: India
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Four folklore medicinal plants Solanum trilobatum, Spathodea campanulata, Syzygium jambos and Tylophora indica leaf extracts were investigated for their antiplasmodial activity in Plasmodium berghei infected Swiss albino mice model. In the Peter’s four day test significant parasite suppression 97.72% (p<0.001) was observed in chloroquine (CQ) control group prolonging the mean survival time of animals for 32 days. An effective parasite suppression (p<0.01) of 72.72% and 63.63% was identified in TICLE and SJCLE respectively at 400 mg/kg b.wt, whereas the SJMLE exhibited 61.36% chemosuppression accomplishing a statistical significance of p

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PC-0000410
Title: Computer aided design and molecular docking studies on a series of 1,3-thiazolidine-2,4-diones as new class of 5-lipoxygenase inhibitors
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Molecular docking study was performed on a series of 25 1,3-thiazolidine-2,4-diones CHP1-CHP25 as potential 5-lipoxygenase (5-LO) inhibitors. The docking technique was applied to dock a set of representative compounds within the active site region of 3V99 (5-LO) using Molegro Virtual Docker v 4.0. For these compounds, the binding free energy (kcal/mol) was determined. The docking simulation clearly predicted the binding mode that is nearly similar to the crystallographic binding mode with 1.38Ao RMSD. Based on the validations and hydrogen bond interactions made by R substituents were considered for evaluation. The results avail to understand the type of interactions that occur between designed ligands with 3V99 binding site region and explain the importance of R substitution on sulfonylureachalcone basic nucleus.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PC-0000409
Title: Synthesis, characterization and biological evaluation of some new chalcones and 2-pyrazolines as potential cytotoxic agents
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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This paper describes the synthesis, characterization and biological evaluation of some new chalcones and 2-pyrazolines. Since these classes of compounds were reported to possess various biological activities, all of these compounds were screened for their cytotoxicity i.e. anticancer potential.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PA-0000408
Title: Rational design and molecular docking studies on a series of 1,3,5-triazine-chalcone hybrids against selected antitubercular drug targets
Category: Pharmaceutical Analysis
Section: Research Article
Country: India
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In the current scenario of drug discovery and development there is an accumulation of diseases through more-complex pathological mechanisms, for which the classic ‘single target, single drug’ pattern has moderately or completely ineffective. In these conditions, drugs acting on various targets could offer greater efficacy profiles compared with single-target drugs. Hence in the present research as a part of our ongoing search for new chemical entities as potential antitubercular agents, we could design a series of 1,3,5-triazine-chalcone hybrid molecules to study their inherent mechanism against selected antitubercular drug target by using molecular docking studies.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PHDRS-0000407
Title: Hepatoprotective role of ethanolic extract of Phyllostachys nigra in experimental-induced hepatotoxicity
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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The purpose of the present study was to assess the Hepatoprotective potential of ethanolic extract of leaves of Phyllostachys nigra on Paracetamol-induced hepatotoxicity. Variable doses of 250 mg/kg and 500 mg/kg of EEPN were administered orally for evaluating their hepatoprotective effect on PCM-induced hepatotoxicity in rats. Both doses (250 mg/kg/p.o. and 500 mg/kg/p.o.) were found to reduce the hepatotoxicity in rats. The increase in the levels reduced glutathione (GSH), tissue nitrite/nitrate and decrease in lipid per oxidation showed the antioxidant activity of the Phyllostachys nigra. Pre-treatment with EEPN reduced the biochemical markers of hepatic injury like serum glutamate pyruvate transaminase (SGPT), serum oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), total bilirubin (TBIL) and total protein shows hepatoprotective activity. It was also found that EEPN at a dose of 500 mg/kg/p.o. was more potent as compared to EEPN (250 mg/kg/p.o.). The study validates scientifically the widely claimed use of Phyllostachys nigra as an ethanomedicine to treat hepatotoxicity.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PC-0000406
Title: New route for the synthesis of unsubstituted dibenzoyl methane and some derivatives of dibenzoyl methane and deoxybenzoins
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Aim: Dibenzoylmethane is a non-toxic, heat stabilizer and one of its derivative - avobenzone has found application as sunscreens. Deoxybenzoins are potent antioxidants and tyrosinase inhibitors with potential to be developed as food preservatives and cosmetic ingredients. In view of this it was worth synthesizing more derivatives from them. Method: Dibenzoylmethane and deoxybenzoins have been treated with dimethyl sulphoxide and acetic anhydride reagent under varying temperature conditions in order to get more derivatives. Result: The interaction of dimethyl sulphoxide and acetic anhydride reagent with dibenzoylmethane (1) gives (2) (3),(4 ) and with deoxybenzoins (5) and (6) gives (7) ,(9), (10), (11) (12) and (13). Conclusion: A number of compounds were synthesized which can have a number of applications.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PC-0000405
Title: Stability study of Gynura procumbens extracts using High Performance Liquid Chromatography
Category: Pharmaceutical Chemistry
Section: Editorial
Country: India
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Background: Gynura procumbens (Lour.) Merr. (Compositae) locally known as sambung nyawa is cultivated in Malaysia and have been traditionally used for various diseases. Accelerated stability studies normally conduct to investigate the chemical kinetics such as rate constant of the reaction, order of the reaction and shelf life of the subject. Objectives: This study was aimed to carry out accelerated stability studies on both 25% ethanolic-aqueous and 95% ethanolic extracts of G. procumbens. Methods: High performance liquid chromatographic method has been developed for the determination of marker compounds in the extracts which then were applied for the stability study. The stability study was conducted following International Council on Harmonization (ICH) guidelines. Samples were exposed to three different storage conditions. Results: The extracts were more stable at 25°C compared to other temperatures studied and the estimated shelf life (t90) of 25% ethanolic-aqueous extract of G. procumbens (25EAEGP) and 95% ethanolic extract of G. procumbens (95EEGP) were 15.91 and 14.08 months, respectively, at 25°C. The marker compounds followed the first order degradation and their degradation was found to be faster at higher temperature and humidity. Conclusion: Thus, for longer shelf life, it is recommended that G. procumbens extracts be stored at temperature of 25°C or below in a tightly closed, dry container.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PC-0000404
Title: Ethyl caffeate ether derivatives as future potential drug
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Ethyl (E)-3-(3,4-dihydroxyphenyl)prop-2-enoate were treated with various alkyl halides in presence of K2CO3 and acetone to obtain their ether derivatives. Further methyl and ethyl ethers were also obtained using DMS and DES respectively. The structure of these compounds were confirmed by IR, NMR and Mass Spectroscopy. All the compounds give satisfactory elemental analysis. Few of them showing promising Antibacterial activity.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PCS-0000403
Title: Development of promising fast dissolving tablets of Stiripentol: a novel antiepileptic drug
Category: Pharmaceutics
Section: Research Article
Country: India
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Stiripentol (STP) is a novel orphan drug which is used as adjunctive therapy for the treatment of severe myoclonic epilepsy in infancy (SMEI, Dravet syndrom). Due to sudden onset of epileptic attack, it is necessary to formulate antiepileptics into such a delivery system, which provides immediate relief. For elderly and pediatrics, dysphagia (difficulty in swallowing) is a common complain. Mouth dissolving tablets formalize an innovative dosage form that conquer the swallowing problems and provide a quick onset of action. The purpose of the present research work was to develop and optimize the formulation of fast dissolving tablets of Stiripentol. Fast dissolving tablets of Stiripentol were prepared by direct compression method using different types of superdisintegrants such as croscarmellose sodium and sodium starch glycolate. The powders were evaluated for angle of repose, compressibility index and hausner's ratio. The prepared tablet formulations were evaluated for hardness, friability, wetting time, drug content, disintegration, water absorption ratio, weight uniformity and the drug release. The results revealed that the formulation F9 which containing a combination of 6% sodium starch glycolate and 15% croscarmellose sodium as superdisintegrants has good dissolution profile and rapid release with shortest disintegration time. It can be concluded that the fast dissolving tablets of the poorly soluble drug, stiripentol, showed enhanced dissolution which could lead to improved its bioavailability and effectiveness.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-CPRMS-0000401
Title: Quality of life in families of children with autism spectrum disorder in India
Category: Clinical Pharmacy and Related Medical Science
Section: Research Article
Country: India
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Background: Autism Spectrum disorder is a complex developmental disability which is fast growing in nature across the globe. Disabilities in children cause not only financial burden but also leads to incomplete state of wellbeing in physical health, psychological health and social health of the families. Aims of this study were to evaluate the QOL in parents of Children with autism, physically disabled as compared to a control group; to compare the QOL of parents of children with autism as per the severity of condition. Methods: The sample consisted of 140 parents (73 mothers and 67 fathers) of 54 Children with autism, 38 children with physical disabilities and 48 healthy children. Diagnostic assessment scales Indian Scale for Assessment of Autism and Functional Ambulation Classification system were used and Quality of life was assessed by the WHOQOL-BREF questionnaire. Results: One way ANOVA and Post Hoc test were used to identify the significant difference of quality of life scores. Parents with Children with Autism Spectrum disorder showed significantly lower quality of life compared with parents of healthy children and parents of children with physical disabilities (p<0.01) in all the four domains of WHOQOL-BREF. Little differences were observed in between physical disability group and normal group in Physical domain and psychological domain, however significant difference was found in social and environmental domain of WHOQOL-BREF. Conclusion: Compared with parents of children with physical disability and normal children, the parents of children with autism spectrum disorder had shown significant impairment in all the four domains (Physical, Psychological, Social and Environmental) measured by WHOQOL-BREF. These findings would help the health care professionals to develop appropriate supportive groups and psycho social intervention programs for the families of children with autism spectrum disorder.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PCS-0000230
Title: Preparation of nanoparticles of Candesartan cilexetil by use of supercritical fluid technology and their evaluation.
Category: Pharmaceutics
Section: Research Article
Country: India
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Candesartan cilexetil is a poorly soluble antihypertensive drug with 15% bioavailability. Various methods are reported to improve the solubility of poorly soluble drugs, one of which is use of supercritical fluids for micronisation of drugs. In the present study, we tried the suitability of supercritical antisolvent precipitation method for preparation of nanoparticles of drug to improve the solubility and dissolution . Nanoparticles were prepared by using different solvents at different temperatures and studied for percentage yield, particle size, solubility and dissolution in different medias such as water , 0.1 N HCl,phosphate buffer pH 6.8 and medium suggested by office of generic drugs. Mean particle size of nanoparticles was found to be around 1776 nm with an advantage of narrow particle size distribution but yield was found to be very low. Considerable increase in saturation solubility was observed as compared to that of pure drug in all medias mentioned above.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-P'Col-0000229
Title: Neuroprotective Effects of Cromakalim on Cerebral Ischemia-Reperfusion (IR) Injury In Streptozotocin (STZ) Induced Type-I Diabetic Rats
Category: Pharmacology
Section: Research Article
Country: India
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Objective: To evaluate neuroprotective effects of cromakalim in diabetic rats. Methods: Wistar rats were randomly assigned to 6 groups (n = 36): Normal control, diabetic (STZ) control, diabetic Sham operated, ischemia reperfusion (IR) control, IR-diabetic control and IR-diabetic with cromakalim. Cromakalim 10 mg/kg intraperitonially (i.p.) was administered daily for 28 days in middle cerebral artery occluded rats treated with STZ. At 24 hours post-surgery, neurological score, brain hemisphere weight difference, lipid peroxidation, Na+/K+ ATPase pump activity and super oxide dismutase level were measured. Results: Following cerebral ischemia-reperfusion injury along with STZ, neurological score, brain hemisphere weight difference, malondialdehyde (MDA) levels were significantly high while Na+/K+ ATPase pump activity and super oxide dismutase(SOD) levels were significantly lower in diseased animals (P < 0.05). Treatment with cromakalim significantly reduced neurological score, brain hemisphere weight difference, malondialdehyde levels while raised Na+/K+ ATPase pump activity and super oxide dismutase levels (P < 0.05). Conclusion: Hence of results our study demonstrated the neuroprotective potential of cromakalim in cerebral IR injury in type I diabetic rats.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-ND-0000228
Title: Quantification of Amentoflavone in two species of Biophytum DC. (Oxalidaceae) using HPLC
Category: Natural Drugs
Section: Research Article
Country: India
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Biophytum is an important medicinal herb used both in traditional systems of medicine as well as in Ayurveda. The present investigation is concerned with the quantification of potential bioactive compound, amentoflavone, in ethanol extracts of two species of Biophytum, Biophytum veldkampii Shanavas et al. and Biophytum reinwardtii (Zucc.) Klotzsch., using HPLC. The study revealed highest amount of amentoflavone (17.18 mg/gm extract) in B. reinwardtii stem compared to all other samples studied. This was followed by B. veldkampii root (3.74 mg/gm) and B. reinwardtii root (3 mg/gm). In remaining parts, it was quantified to be less than 2 mg/gm. On the whole, these two species are rich sources of amentoflavone, the potent bioactive compound. Since both the species are very common in almost all habitats with moist soils such as wastelands, fields, forest slopes, large scale isolation of amentoflavone appears to be an economically viable possibility.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-MB-0000227
Title: In vitro inhibition of selected human fungal pathogens with various seed extracts of Anamirta cocculus (Linn.)
Category: Microbiology
Section: Research Article
Country: India
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Objective: To screen the antifungal activity of seed extracts of Anamirta cocculus (Linn.) against twelve pathogenic fungi. Methods: The endosperms from the seeds were extracted with petroleum ether, benzene, chloroform, acetone, methanol, ethanol, water and a mixture of methanol and ethyl acetate (1:3) solvents by using Soxhlet apparatus for organic solvents and cold extraction procedure for aqueous solvent. The antifungal activity of all the solvent extracts was evaluated against selected fungal strains of Aspergillus spp., Fusarium spp., Botrytis cinerea, Trychophyton mentagrophytes, Epidermophyton floccosum and four Candida spp. The zone of inhibitions and minimum inhibitory concentrations (MICs) of the extracts were determined by running the experiments in triplicates. Results: All the extracts showed significant levels of antifungal activities (zone of inhibition 5±0.3 to 16±0.4mm). The most active extract against all the tested fungal strains was methanol extract with 16±0.4mm growth inhibition zone against A. niger at 100 mg/mL concentration. MICs of the extracts also revealed methanol extract as the most potent one with a lowest inhibitory concentration of 30 mg/mL against A. niger. Conclusions: The findings of the present study indicated that the seed extracts of A. cocculus could be an alternative to synthetic fungicides and may be used in developing novel types of natural fungicides.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PC-0000216
Title: Triazolo aspirins inhibit proliferation of C6 glioma cell lines in vitro
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Background: 1, 2, 3 triazole based molecules are emerging as promising anticancer drugs. Aspirin inhibits cell proliferation and induces apoptosis in various cancer cell lines. We have synthesized novel conjugates incorporating both aspirin and triazole ring systems. Gliomas are the most devastating intracerebral neoplasms that occur due to transformation of astrocytes. Deregulation of NF-κB signaling pathway adds to the enriched glioma persistence, proliferation and chemo resistance. Hence, the present study was carried out to test the efficacy of these compounds against the growth of C6 glioma cell lines in vitro. Methods: Triazolo aspirins were synthesized by regioselective Cu (I)-catalyzed azide alkyne cycloaddition. The structures of these compounds were characterized by IR, 1HNMR, 13C NMR, mass spectrometric data and micro analysis. The compounds were tested for their efficacy to inhibit the growth of C6 glioma cell lines in vitro. Results: Among the triazolo aspirins investigated, dioxoisoindolin-butyl-triazole (abbreviated as TIBMAB) showed significant activity with IC-50 of 100±60 ìM. The anti-proliferative activity through MTT and Clonogenic assay indicated decreased viability, FACS studies showed cell cycle G2/M-phase arrest, TUNEL studies exhibited that many cells were undergoing apoptotic cell death, immunofluorescence studies displayed ability of TIBMAB to inhibit the TNF-α induced translocation of p65 into nucleus, western blot analysis confirmed TIBMAB induced apoptosis through activation of caspase-3 along with enriched PARP cleavage and inhibited the TNF-α induced translocation of NF-κB into nucleus. Conclusion: TIBMAB showed substantial inhibition of C6 glioma cell line proliferation and decreased cell survival partly by inhibiting the NF-κB signaling.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PB-0000215
Title: Enzymatic function prediction for the hypothetical proteins of V. cholerae O395 by conserved domain analysis
Category: Pharmaceutical Biotechnology
Section: Research Article
Country: India
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Vibrio cholerae is a bacterium known for spreading cholera disease in humans. This study investigated enzymatic function in the hypothetical proteins coded by V. cholerae. Four conserved domain analysis web tools used as CDD BLAST, INTERPROSCAN, PFAM, and CATH along with PS2 protein structure prediction server. These web tools successfully predicted the function and structure of hypothetical proteins. The results of V. cholerae hypothetical proteins were documented for coding enzymes. Further investigation is needed to understand the role of these hypothetical proteins in metabolic pathway which will be useful for bio-controlling.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PB-0000214
Title: A calibrated protocol for direct regeneration of multiple shoots from in vitro apical bud of Ocimum basilicum - An Important Aromatic Medicinal Plant
Category: Pharmaceutical Biotechnology
Section: Research Article
Country: India
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The chief purpose of the present investigation was to develop a repeatable protocol for rapid clonal multiplication using in vitro apical bud of Ocimum basilicum for commercial purpose and mass cultivation of diseases free plants. Plant regeneration from cultured in vitro apical bud explants of Ocimum basilicum was obtained by direct shoot development on Murashige and Skoog’s basal medium supplemented with BAP (8.88 µM) and Kinetin (9.28 µM). MS + BAP (8.88 µM) was found to be the most suitable medium for initiation and multiplication of shoots from apical bud. Regenerated shoots were grown on the same medium for further development, 74 days old culture when sub cultured on the same medium exhibited large number of healthy multiple shoots of 6-8 cms in height .These 15-20 healthy multiple shoots developed roots on the same media thus avoiding an additional step of in vitro rooting. Complete plants thus obtained were transferred to sterilized soil in plastic pots for 4-6 weeks and then to field.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PHDRS-0000213
Title: Free radical scavenging & Iron chelating capacity of Psidium guajava and Persea americana leaf extract: A comparative study
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Background: Oxidative stress is caused due to the discrepancy between reactive oxygen and nitrogen species with antioxidant system in the body. Free radicals which are produced endogenously destroy lipids, proteins and DNA biomolecules which results in cancer, ageing, autoimmune, cardiovascular and neurodegenerative disorders. Phytochemical constituents of plants such as Phenolic compounds, Flavonoids, Saponins and alkaloids act on free radicals which reduce oxidative stress induced complications. Present study was designed to compare the Free radical scavenging and iron chelating capacity of Psidium guajava and Persea americana leaf extracts. Methods: Ability to scavenge Hydroxyl radicals which was produced by Fenton reaction as described by Naskar et al26, using qurecetin as a standard. Iron chelating capacity of plant extract was determined by modified method described by Dinis et al25, using Disodium EDTA as standard. Results: At 10 µg concentration Psidium guajava and Persea americana displayed 77.92 ± 1.8 & 65.99 ± 4.17 % hydroxyl radical scavenging activity respectively, as compared to standard Qurecetin which displayed 89.84 ± 0.89 % scavenging activity. A dose dependent increase in scavenging activity was observed in the crude extracts. Chelating activity of plant extract was also observed to be increased in a dose dependent manner. At 10 µg concentration Psidium guajava and Persea americana displayed 57.49 ± 0.9 & 54 ±2.3% iron chelating activity respectively, as compared to standard Disodium EDTA that displayed 75.44 ± 0.33 % iron chelating activity. Conclusion: The results obtained from present in vitro study indicates that Psidium guajava and Persea americana are potential source of natural antioxidants. Psidium guajava leaf extract exhibited better in vitro antioxidant activity when compared to Persea americana.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PC-0000212
Title: Structural Elucidation of Potential Impurities of Raloxifene Hydrochloride by LC/ESI-MS and NMR#
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Three impurities were detected during the impurity profile study of raloxifene hydrochloride by reverse phase gradient high performance liquid chromatographic method (HPLC) whose area percentage ranged from 0.06 to 0.15%. The product ion spectrum of these impurities and Raloxifene was recorded in LCMS and the fragmentation patterns of these impurities were observed and these three impurities displayed identical molecular ion at m/z 488. A thorough study was undertaken to characterize these impurities. They were isolated from enriched mother liquors by preparative HPLC. Based on the spectral data (1H,13C NMR, 1D NOE, 2D NMR and Mass), the structure of these impurities 1-3 were characterized as 1-(2-(4-(6-hydroxy-2-(4-hydroxyphenyl)benzo[b]thiophene-3 carbonyl) phenoxy) ethyl)-1-methylpiperidin-1-ium (Impurity-1), [6-Hydroxy-2-(4-methoxy phenyl)-benzo[b]thiophen-3-yl]-[4-(2-piperidin-1-yl-ethoxy) phenyl]-methanone (Impurity-2) and [2-(4-hydroxy-phenyl)-6-methoxy-benzo[b]thiophen-3-yl]-[4-(2-piperidin-1-yl-ethoxy)-phenyl]-methanone (Impurity-3).

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PHDRS-0000211
Title: Some herbal drugs used for treatment of diabetes
Category: Pharmacognosy and Herbal Drugs related study
Section: Review Article
Country: India
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Diabetes mellitus is a dreadful disease found in all parts of the world and is becoming a serious threat to mankind health. Diabetes mellitus is a group of metabolic diseases characterized by high blood sugar (glucose) levels that result from defects in insulin secretion, or action, or both. The World Health Organization (WHO) has listed 21,000 plants, which are used for medicinal purposes around the world. Herbal medicines have been highly esteemed source of medicine throughout the human history. Alternative to synthetic agents, plants provide a potential source of hypoglycemic drugs and are widely used to prevent diabetes. In the present review, an attempt has been made to summarize some of the herbal plants having anti-diabetic activity which are beneficial for the mankind.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-PP-0000210
Title: Optimization of SFE extraction of three sesquiterpenes from Syringa pinnatifolia Hemsl using orthogonal test design and subsequent isolation by HSCCC chromatography
Category: Pharmacognosy and Phytochemistry,
Section: Research Article
Country: India
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Objective: In the present study, we investigated the Optimization of SFE extraction of three sesquiterpenes from Syringa pinnatifolia Hemsl using orthogonal test design and subsequent isolation by HSCCC chromatography. Methods: An orthogonal L9(3)4 test design was applied to select the optimum extraction parameters including pressure, temperature, modifier and sample particle size on yield using an analytical-scale SFE system. The crude extract from preparative SFE was successfully isolated and purified by preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane–chloroform–methanol–water (10:11:11:10, v/v) by increasing the flow-rate of the mobile phase stepwise from 1.2 to 2.2 ml/min after 120 min. Results: The process was scaled up by 80 times using a preparative SFE system under the optimized conditions of 20 Mpa of pressure, 30 °C of temperature, 40-60 mesh of sample particle size and modified CO2 with 20% methanol. The target compounds isolated and purified by HSCCC were analyzed by high-performance liquid chromatography (HPLC). The separation produced total of 50.8 mg of 15-norcadina-2-en-5,9-diol at 99.0% purity, 35.4 mg of guai-9-en-4â-ol at 98.5% purity and 39.0 mg of 14,15-dinorguai-1,11-dien-9,10-dione at 98.8% purity from 200 mg of the crude extract in one-step separation. Conclusion: The sesquiterpenes were isolated from Syringa pinnatifolia Hemsl may serve as a potential source of useful drugs in the near future.

Journal: Journal of Pharmacy Research , Volume: 8, Issue: 6.
Article Id: JPRS-QA-0000209
Title: Drug Impurity Profiling: A Scientific Approach
Category: Quality Assurance
Section: Review Article
Country: India
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There is an ever increasing interest in impurities present in APIs. Now days, not only purity profile but also impurity profile has become mandatory according to various regulatory authorities. In the pharmaceutical world, an impurity is considered as any other inorganic or organic material, or residual solvents other than the drug substances, or ingredients, arise out of synthesis or unwanted chemicals that remains with APIs. The impurity may be developed either during formulation, or upon aging of both APIs and formulated APIs in medicines. The presence of these unwanted chemicals, even in small amount, may influence the Identity, Strength, Safety, Purity, Quality and Efficacy of the pharmaceutical products. The different Pharmacopoeias, such as the British Pharmacopoeia (BP), United States Pharmacopeia (USP), and Indian Pharmacopoeia (IP) are slowly incorporating limits to allowable levels of impurities present in the APIs or formulations. As per ICH, Impurity profiling is a group of analytical activities, with aim of isolation, structure elucidation, identification and quantitative determination of organic and inorganic impurities and residual solvents in bulk drugs & pharmaceutical formulations. Impurity is any component of the drug substance or product that is not the chemical entity of it, or is any substance coexisting with the original drug, like starting materials or intermediates or that is formed, due to any side reactions. The ICH has published guidelines on impurities in new drug substances (ICH, Q3A), products (ICH, Q3B), and residual solvents (ICH, Q3C). Impurity profiling study has been in the limelight in the recent pharmaceutical scenario and its importance is increasing day-by-day.

Cite this article as: Gauri P. Jadhav, Veena S. Kasture, Sarita S.Pawar, Anuja R. Vadgaonkar, Ashish P. Lodha,Snehal K. Tuse, Harshal R. Kajale, Santosh A.Borbane,Drug Impurity Profiling: A Scientific Approach,Journal of Pharmacy Research 2014,8(6),696-706.