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Journal of Pharmacy Research
ISSN NO: 0974-6943
The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified when, we/or author get comments from any readers. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry,  Nursing, Paramedical, prescription etc  fields).
Scopus Indexed (link http://www.scimagojr.com/journalsearch.php?q=21100325431&tip=sid&clean=0) 

Journal Metrics for this Journal of Pharmacy Research (Source ID: 21100325431): 2015(SNIP) Source Normalized Impact Per Paper : 0.575; SCImago Journal Rank (SJR):0.787; Impact Per Publication : 0.789 CiteScore 2016: 0.93(Top level : Life Science)

Year SJR Cites per document Year Value
2014 0.607 Cites / Doc. (4 years) 2014 0.607
2015 0.787 Cites / Doc. (4 years) 2015 0.789
2016 0.926 Cites / Doc. (4 years) 2016 0.926
    Cites / Doc. (3 years) 2014 0.607
    Cites / Doc. (3 years) 2015 0.789
    Cites / Doc. (2 years) 2014 0.607
    Cites / Doc. (2 years) 2015 0.789
2016   Cites / Doc. (4 years) 2016 0.926
Cites Year Value
External Cites per document 2014 0.607
External Cites per document 2015 0.789
External Cites per document 2016 0.926
Cites per document 2014 0.607
Cites per document 2015 0.789
Cites per document 2016 0.926

Manuscripts Published

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PA-0000336
Title: Analytical Method Development and Validation for the Estimation of Ursodeoxycholic Acid using RP-HPLC.
Category: Pharmaceutical Analysis
Section: Research Article
Country: India
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The Present work is undertaken with an aim to develop and validate an accurate, precise and rapid method for the estimation of Ursodeoxycholic Acid in tablet and bulk dosage form. The method is simple, accurate and sensitive. The separation was achieved on a gradient high pressure liquid chromatograph (Jasco, RI 2031 Plus) pumps Jasco PU 2080 Plus, IR detector column oven (Jasco), system controller (Jasco), a disposable guard column LC-18 (PELLIGUARD)TM , LC-18, 2 cm, supelco, inc., Bellefonte, and a Reverse Phase C-18 (phenyl) Column (25 cm x 4.6 mm) i.d., particle size 5 µm) was used The HPLC system was equipped with the software class, Borwin (Jasco). Flow Rate : 1.0 ml/min Injection Volume : 10µl Run Time : 15 min, Detector temp: 40 oC. The method was validated for specificity, precision, linearity, and accuracy. The recovery range for baclofen is in the range of 99.0–102.0% and the method can be successfully applied for the routine analysis of the drug substance.

Cite this article as: Soni Varinder, Parminder Kumar, Saini Gurjeet,Gagan shah, R.K Dhawan,Analytical Method Development and Validation for the Estimation of Ursodeoxycholic Acid using RP-HPLC,Journal of Pharmacy Research 2015,9(1),46-53.

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-MPS-000083
Title: Benzoic acid, one of the major components of aqueous bark extract of Terminalia arjuna protects against Copper-Ascorbate induced oxidative stress in human placental mitochondria through antioxidant m
Category: Mechanism / Pathway Study
Section: Research Article
Country: India
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Background: Very recently, we have shown that a number of compounds isolated from plants and their parts having medicinal importance are capable of providing protection against copper-ascorbate induced oxidative stress mediated damages in isolated mitochondria in vitro. The results demonstrated that these compounds appear to be potentially effective in ameliorating mitochondrial dysfunction associated with oxidative stress. In the present study, we investigated, in individual experiments, the protective effects of the aqueous bark extract of Terminalia arjuna (TA) or benzoic acid, as its major constituent, against copper-ascorbate induced oxidative stress mediated damages to mitochondria obtained from human placenta, in vitro. Methods: In this study, the antioxidant property of TA was established by hydroxyl radical, superoxide radical and nitric oxide scavenging activities. The composition of the aqueous bark extract of TA was analyzed by GC-MS analysis. Human placental mitochondria were used as a model organelle to investigate whether the aqueous bark extract of TA or benzoic acid protects against copper-ascorbate-induced oxidative stress, in vitro, by spectro-photometrically measuring the levels of biomarkers of oxidative stress, the activities of antioxidant enzymes and the enzymes associated with energy metabolism, and evaluating the status of morphology of mitochondria through studies using scanning electron microscopy. Results: In separate experiments, aqueous bark extract of TA or benzoic acid were found to protect against copper-ascorbate-induced alterations in the level of biomarkers of oxidative stress in a dose-dependent manner. Moreover, the activities of antioxidant enzymes also were protected from being altered by aqueous bark extract of TA or benzoic acid. The alterations in conjugated diene level, tryptophan and di-tyrosine fluorescence, mitochondrial swelling, thiolase activity and NADH autofluroscence, mitochondrial membrane potential and morphology were found to be protected when the mitochondria were co-incubated with copper-ascorbate and the aqueous bark extract of TA or with copper-ascorbate and benzoic acid in a dose-dependent manner. Although the aqueous bark extract of TA or benzoic acid independently can protect the placental mitochondrial from copper-ascorbate-induced oxidative stress mediated damages but the degree of protection provided by benzoic acid was found to be more compared to the aqueous bark extract of TA. Conclusion: Based on these findings it can be concluded that the aqueous bark extract of TA containing benzoic acid as its major constituent may serve as a potential medicinal resource for future use in situations associated with oxidative stress.

Cite this article as: Mousumi Dutta, Aindrila Chattopadhyay,Arnab Kumar Ghosh, Utpal Roy Chowdhury, Debajit Bhowmick, Biswajyoti Guha, Tridib Das, Benzoic acid, one of the major components of aqueous bark extract of Terminalia arjuna protects against Copper-Ascorbate induced oxidative stress in human placental mitochondria through antioxidant mechanism(s): an in vitro study,Debasish Bandyopadhyay1, #,*Journal of Pharmacy Research 2015,9(1),64-88.

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-ORS-000078
Title: Endothelin-1 in Periodontal Diseases: A Mini-Review
Category: Others ( Related Science )
Section: Review Article
Country: India
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Endothlin-1 (ET-1) is a 21 amino acid peptide and is a potent vasoconstrictor produced by endothelial cells. It plays a role in the development of diseases such as hypertension and atherosclerosis. Reports have suggested the identification of ET-1 in chronic periodontitis and gingival overgrowth. Thus, there is a need to appraise the role of ET-1 in periodontal disease. Further research is needed to confirm its use as a biomarker and to incorporate endothelin-antagonists in periodontal therapy.

Cite this article as: Dr. Waleed Khalid, Dr. Sheeja S.Varghese, Dr. Reema Lakshmanan3, Dr. Sankari M., Dr.N.D. Jayakumar,Endothelin-1 in Periodontal Diseases: A Mini-Review,Journal of Pharmacy Research 2015,9(1),31-34.

 

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PC-000077
Title: ICP – OES investigations of heavy metal contents in cosmetic products
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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A variety of chemicals especially heavy metals used in cosmetics as ingredients has different health effects. The level of heavy elements present viz., arsenic, cadmium, chromium, mercury, nickel, lead, cobalt, copper and zinc in twenty different cosmetic samples such as Deodorant (One – D 1), Eyeshades (Three – E 1 – 3), Face cream (Three – F 1 – 3), Hair dye (Two – H 1 – 2), Kohl (One – K 1), Lipstick (Four – L 1 – 4), Soap (One – S 1), and Talcum Powder (Five – P 1 – 2; R 1 – 3) were studied. All the samples were digested by suitable protocols and the composition of heavy metals analyzed, quantified on an ICP-OES. The results show that considerable amount of such elements were present in the test samples. As high as 0.523 ppm of arsenic is found in hair dye. Chromium varies from 0.011 ppm to 0.421 ppm, nickel between 0.015 ppm to 0.139 ppm.1.458 ppm of lead found as maximum in hair dye and minimum of 0.05 ppm found in eye shade. The samples viz., lipstick, soap, deodorant and powder contain lead at BDL. The amount of cobalt ranges between 0.01 ppm and 0.044 ppm. Copper is found between 0.014 ppm and 0.086 ppm. All the test samples contain considerable quantity of zinc at levels between 0.108 ppm and 126.6 ppm. The investigated results indicate that prolonged use of cosmetics pose threat to human health and the environment.

Cite this article as: Rajagopal Mayildurai, Alagunambi Ramasubbu, Nanjan Velmani,ICP – OES investigations of heavy metal contents in cosmetic products,Journal of Pharmacy Research 2015,9(1),27-30.

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-P'Col-000076
Title: Evaluation of In vitro Antioxidant Activity of Aeschynomene indica
Category: Pharmacology
Section: Research Article
Country: India
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Background: A number of Indian medicinal plants have been used for thousands of years in the traditional system of medicine. Methods: The whole plant of Aeschynomene indica was successively extracted using soxhlet apparatus with solvents as petroleum ether (600C-800C), chloroform, acetone, ethyl acetate and ethanol. The antioxidant activity was studied by using 2,2-diphenyl-p-picryl hydrazyl (DPPH) and free radical scavenging activity by hydrogen peroxide scavenging activity. Results and Discussion: The extracts showed various yields, the ethyl acetate and ethanolic extract yields are 12.64% and 14.08% respectively. The TLC based qualitative analysis showed the presence of flavonoids of and phenolic compounds. The flavonoids from the ethyl acetate and ethanolic extracts have showed good responses towards antioxidant activity comparing with the other remaining extarcts. Conclusion: The IC50 for ethyl acetate and ethanolic exracts were found to be 66.108±0.402 and 108.28±0.621 μg/ml respectively in DPPH method. The IC50 for ethyl acetate and ethanolic extracts were found to be1255.16±0.362 and 177.01±0.462μg/ml respectively in Hydrogen Peroxide method.

Cite this article as: M. Prathibha Bharathi ,D.Muralidhara Rao, Evaluation of In vitro Antioxidant Activity of Aeschynomene indica,Journal of Pharmacy Research 2015,9(1),21-26.

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PC-000075
Title: In silico screening of 3,4 dihydropyrimidones as focal adhesion tyrosine kinase inhibitors
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Credible role  in metastasis and over expression in invasive tumors made Focal adhesions tyrosine kinase  [FAK] a target for the development of anticancer drugs. Cancers of the thyroid, prostate, cervix, colon, rectum, oral epithelium and ovary have shown increased in FAK levels. In search for new drugs structure-based design is an important technique applied currently in order to speed  up the lead finding and optimization In this study, Molecular modeling and docking analysis were used to predict and understand interactions between Focal Adhesion Kinase and twenty four compounds of 3,4 dihydropyrimidones. 3BZ3 atomic coordinates of Focal Adhesion Kinase was retrieved from protein data bank (PDB). All ligands were drawn by software chemsketch . Molegro virtual docker program that predicted interactions in terms of Dock score. The approach is applicable in engineering 3D structures of enzymatic models, and studying interactions of active site residues with ligands show that the three compounds: it is concluded that that 5-(1H-benzimidazol-2-yl)-4-(4-hydroxy-3-methoxyphenyl)-6-methyl-3,4-dihydropyrimidine-2(1H)-one, 5-(1H-benzimidazol-2-yl)-4-(4-hydroxyphenyl)-6-methyl-3,4-dihydropyrimidin-2(1H)-one and 5-(1H-benzimidazol-2-yl)-4-(1H-indol-3-yl)-6-methyl-3,4-dihydropyrimidin-2(1H)-thione could be a potent anticancer target molecule against Focal adhesions tyrosine kinase which may be worth for further clinical trials.

Cite this article as: Gopinathan Narasimhan,Chitra Krishnan,In silico screening of 3,4 dihydropyrimidones as focal adhesion tyrosine kinase inhibitors,Journal of Pharmacy Research 2015,9(1),15-20.

 

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PC-000074
Title: Synthesis and preliminary evaluation of some 2-amino-n’-[substituted]-4,5,6,7- tetrahydro-1-benzothiophene-3-carbohydrazide as antimicrobial agents.
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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A new series of 2-amino-N’-[substituted]-4,5,6,7-tetrahydro-1-benzothiophene-3-carbohydrazide 2(a-i) were synthesized using appropriate synthetic route. The entire test compounds were assayed in vitro for antibacterial activity against two different strains of Gram-positive (S. aureus, B. subtilis and S. faecalis), Gram-negative (E. coli, P. aeruginosa and S. typhi) bacteria and the antifungal activity against (C. albicans, A. flavus and A. niger) fungi. The minimum inhibitory concentration (MIC) was determined for test compounds and for reference standards. The test compounds showed significant antibacterial and antifungal activity against the microbial strains used, when tested in vitro.

Cite this article as:V. A.Jagtap and Y. S. Agasimundin,Synthesis and preliminary evaluation of some 2-amino-n’-[substituted]-4,5,6,7-tetrahydro-1-benzothiophene-3-carbohydrazide as antimicrobial agents. Journal of Pharmacy Research 2015,9(1),10-14.

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PA-000073
Title: A validated new stability indicating densitometric method for quantitative analysis of glipizide in tablets
Category: Pharmaceutical Analysis
Section: Research Article
Country: India
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A sensitive, precise, specific, linear, and stability-indicating high performance thin-layer chromatographic (HPTLC) method has been established and validated for quantitative analysis of one of the antidiabetic agents, the sulfonylurease glipizide in active pharmaceutical ingredient (API) and in tablet formulation, using toluene: methanol (8:2; v/v) as mobile phase. Chromatographic separation was achieved on precoated silica gel 60F254 plates with 0.2 mm thickness. Detection and quantification were performed by classical densitometry using a UV detector at 230 nm. Compact spots were obtained for glipizide (RF 0.35± 0.02, mean ± SD). Calibration plots were constructed in the range 100-700 ng per band and were linear with good calibration coefficients (r =0.9997 ±0.01, mean ± SD) for glipizide. The limit of detection and quantification were 100 and 300 ng per spot for glipizide. Glipizide was subjected to the International Conference on Harmonization (ICH) prescribed hydrolytic (acid, base, and neutral), oxidative, photolytic, and thermal stress conditions. Among all the above mentioned conditions, the drug was found to be susceptible to some stress conditions. However, the degradation products were well resolved from the pure drugs with significantly different RF values.

 

Cite this article as:Lobhe G. A, Shah Amol, Singhvi Indrajeet, A validated new stability indicating densitometric method for quantitative analysis of glipizide in tablets Journal of Pharmacy Research 2015,9(1),5-9.

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PP-000072
Title: Phytochemistry of some medicinal plants from Western Ghat region
Category: Pharmacognosy and Phytochemistry,
Section: Research Article
Country: India
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Medicinal plants have bioactive compounds which are used for curing of various human diseases. Phytochemicals have two categories i.e., primary and secondary constituents. Primary constituents have chlorophyll, proteins sugar and amino acids. Secondary constituents contain terpenoids and alkaloids. Medicinal plants have antifungal, antibacterial and anti-inflammation activities. The present study involves five different medicinal plants Acacia nilotica, Morus alba, Morus nigra, Momordica charantia, Luffa cylindrical locally available in Gaganbavada Taluka. The results of the phytochemical analysis of these medicinal plants showed that the terpenoids, phlobatannins, reducing sugar, flavonoids and alkaloids were found to be present in these mentioned medicinal plants. The phytochemical analysis of the plants is very important commercially and has great interest in pharmaceutical companies for the production of the new drugs for curing of various diseases. It is expected that the important phytochemical properties recognized by our study in the indigenous medicinal plants of Gaganbavada Talukawill be very useful in the curing of various diseases of this region.

Cite this article as:S. K. Mengane and S. S. Kamble,Phytochemistry of some medicinal plants from Western Ghat region, Journal of Pharmacy Research 2015,9(1),1-4.

 

 

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-QA-000080
Title: Development and validation of an UV spectrophotometric method for simultaneous determination of cilnidipine and chlorthalidone
Category: Quality Assurance
Section: Research Article
Country: India
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A simple, accurate and precise dual wavelength spectrophotometric method was developed for simultaneous determination of Cilnidipine and Chlorthalidone in combined pharmaceutical dosage form. The principle for dual wavelength method is the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest. The wavelengths selected for determination of Cilnidipine are 271.83 nm and 278.34 nm, whereas, the wavelengths selected for determination of Chlorthalidone are 233.83 nm and 250.0 nm. Methanol is used as a solvent. Regression analysis of Beer’s plots showed good correlation in concentration range of 2-10 μg/ml for Cilnidipine and 2.5-12.5 μg/ml for Chlorthalidone. The method was validated according to ICH guidelines for various parameters like accuracy, precision, specificity, linearity, robustness, LOD and LOQ. The linearity of the proposed method was investigated in the range of 2-10 mg/mL (r2=0.9990) for Cilnidipine and 2.5 -12.5mg/mL (r2 = 0.9986) for Chlorthalidone. The limits of detection (LOD) were 0.4174 μg/mL and 0.068 μg/mL, and the limits of quantitation (LOQ) were 1.264 ìg/ml and 0.206 μg/ml, for Cilinidipine and Chlorthalidone respectively The proposed method was successfully applied to determination of these drugs in commercial tablets.

Cite this article as: Snehal N. Patel, Madhuri A. Hinge, Varsha M.Bhanushali,Development and validation of an UV spectrophotometric method for simultaneous determination of cilnidipine and chlorthalidone,Journal of Pharmacy Research 2015,9(1),41-45.

DOI: https://dx.doi.org/10.6084/m9.figshare.3205300.v1

 

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PB-0000142
Title: Fructooligosaccharides - type prebiotic : A Review
Category: Pharmaceutical Biotechnology
Section: Review Article
Country: India
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The main advantage of prebiotic oligosaccharides is that they are natural functional ingredients. Prebiotics are being used in the food industry as functional ingredients in beverages, milk products, probiotic yogurts and symbiotic products. Fructooligosaccharides (FOS) are oligosaccharides that occur naturally in Medicinal plants such as onion, chicory, garlic, asparagus, banana, artichoke, among many others. FOS are not digested by the human gastrointestinal tract, and when they reach the colon, they beneficially stimulate the growth and strengthening of specific bacteria in the intestine. Several studies have demonstrated the functional properties of FOS, such as the reduction of cholesterol levels and blood glucose levels, lowering of blood pressure, better absorption of calcium and magnesium and to inhibit production of the reductase enzymes that can contribute to cancer. Currently FOS are increasingly included in food products and infant formulas due to their prebiotic effect stimulate the growth of nonpathogenic (bifidobacteria) intestinal microflora and decreases growth of potentially pathogenic bugs and enhances the immune system.

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PC-00001140
Title: Synthesis of Novel Trifluorobenzimidazole Derivatives and Their Study of 5-LOX Inhibition and Brine Shrimp Lethal Bioassay (BSLB)
Category: Pharmaceutical Chemistry
Section: Research Article
Country: India
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Here in we design and synthesized novel 2-(trifluoromethyl)-5-methyl-1-((5-aryl-1,3,4-oxadiazol-2-yl)methyl)-1H-benzimidazoles. All the synthesized compounds were well characterized by advanced analytical and spectrometrical methods (MS, 1H and 13C NMR) and evaluated for their 5-Lipoxygenase (5-LOX) inhibitory activity. Bio evaluation confirms that compound 7d exhibiting good inhibition with an IC50 value of 8.59 µg/mL against 5-LOX. In addition, the same compounds were further screened for in vitro cytotoxicity by Brine Shrimp Lethal Bioassay (BSLB). Compounds 7c and 7a showed potent cytotoxicity with an ED50 values 7.39 µg/mL and 7.54 µg/mL respectively.

Cite this article as:Vara Prasad Yenugula, Praveen Choppara, Murthy Y.L.N*.Synthesis of Novel Trifluorobenzimidazole Derivatives and Their Study of 5-LOX Inhibition and Brine Shrimp Lethal Bioassay (BSLB), Journal of Pharmacy Research 2015,9(1),95-100.

 

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PSPA-000084
Title: Phytochemical screening and antioxidant activity of combination of Pleurotus florida and Agrocybe cylindracea
Category: Phytochemical Study and Pharmacological Activity
Section: Research Article
Country: India
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Background: Several mushroom species have been pointed out as sources of antioxidant compounds, in addition to their important nutritional value. Pleurotus florida & Agrocybe cylindracea are among the most studied species all over the world, but those studies focused on their fruiting bodies instead of other presentations, such as powdered preparations, used as supplements. Methods: In the present work the chemical composition and antioxidant activity (free radical scavenging activity, reducing power and lipid peroxidation inhibition) of dried powder formulations of the mushroom species Pleurotus florida & Agrocybe cylindracea (PF and AC, respectively) were evaluated. Results: Powder formulations of both species revealed the presence of essential nutrients, such as carbohydrates, proteins, and unsaturated fatty acids. Furthermore, they present a low fat content (Conclusion: PF showed higher antioxidant activity and higher content of tocopherols and phenolic compounds (124 and 770 lg/100 g, respectively) than LPF (32 and 690 lg/100 g). Both formulations could be used as antioxidant Sources to prevent diseases related to oxidative stress.

Cite this article as: S. Selvakumar , S. Sankar,Phytochemical screening and antioxidant activity of combination of Pleurotus florida and Agrocybe cylindracea,Journal of Pharmacy Research 2015,9(1),89-94

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PHDRS-000082
Title: Pharmacognostic Evaluation and Physiochemical Analysis of the Rhizomes of Arisaema turtuosum Wall.
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Background : Arisaema tortuosum is probably the most common species of genus Arisaema from our part of the world. From the ancient times this plant is being used in ethano-medico practices and for food purposes. It is used for abscess and antinematodal activity .The decoction of tuber is given to animals for early recovery of fractured bone also used internally to cure piles. The tribals used it as antidote for dog bite, to cure liver complaints and stomachache and in various ailments related to digestive tract like constipation, indigestion, abdominal pain and dysentery. Tuber have good asthetic, antihepatotoxic, anticancerous, antimicrobial and antioxidant properties. In view of its medicinal importance and taxonomic validation, pharmacognostical standardization of rhizome of Arisaema tortuosum has been carried out and being presented under this study. Methods: The study includes macroscopical and microscopical evaluation along with estimation of its physicochemical parameters such as ash and extractive values and preliminary phytochemical screening. Result: In initial phytochemical screening, some important constituents like carbohydrate, glycoside and steroids have been found in its extracts. The microscopy of powder shows acicular crystals, xylem vessels, starch grains. Conclusion:The present study reveals standardization profile of Arisaema tortuosum which would be of immense value in botanical identification and authentication of plant drug and may help in preventing its adulteration.

Cite this article as: Hemlata Verma, Dr V.K Lal, Dr. K. K Pant ,Pharmacognostic Evaluation and Physiochemical Analysis of the Rhizomes of Arisaema turtuosum Wall.Journal of Pharmacy Research 2015,9(1),60-63

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-PHDRS-000079
Title: Efficacy of different solvent extracts of Aristolochia krisagathra and Thottea ponmudiana for potential antimicrobial activity
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
Country: India
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Objective: To investigate the antimicrobial potentiality of two medicinal plants namely Aristolochia krisagathra and Thottea ponmudiana against 12 bacteria and 2 fungi. Methods: The Staphylococcus aureus (S. aureus) (MTCC 96), Staphylococcus epidermidis (S. epidermidis) (MTCC 435), Bacillus subtilis (B. subtilis) (MTCC 121), Escherichia coli (E. coli) (MTCC 739), Klebsiella pneumoniae (K. pneumoniae) (MTCC 432), Proteus vulgaris (P. vulgaris) (MTCC 742), Proteus mirabilis (P. mirabilis) (MTCC 425), Salmonella paratyphi A (S. paratyphi A) (MTCC 735), Salmonella paratyphi B (S. paratyphi B) (clinical isolate), Salmonella typhimurium (S. typhimurium) (MTCC 98), Shigella flexneri (S. flexneri) (MTCC 1457), Vibrio parahaemolyticus (V. parahaemolyticus) (MTCC 451), Candida albicans (C. albicans) (MTCC 183) and Cryptococcus neoformans (C. neoformans) (clinical isolate) were originally obtained from Microbial Type Culture Collection Centre (MTCC), Institute of Microbial Technology, Chandigarh, India. The mentioned plants of A. krisagathra and T. ponmudiana were extracted with methanol and petroleum ether extracts and its antimicrobial property was tested by using disc diffusion and broth dilution methods. Results: Antimicrobial potentiality of methanolic and petroleum ether extract of A. krisagathra and T. ponmudiana was evaluated against microorganisms, the highest inhibitory activity was observed against S. aureus (22.8 mm), S. epidermidis and B. subtilis (17.0 mm) in methanolic extract, where as petroleum ether extract showed moderate inhibitory activity against S. epidermidis (17.4 mm) and B. subtilis (16.2 mm). According to broth dilution method, the methanolic plant extract of A. krisagathra showed the highest Minimal Inhibitory Concentration (MIC) values against S. aureus (128 µg/mL), S. epidermidis and B. subtilis (256 µg/mL), whereas the petroleum ether extract of A. krisagathra showed the moderate MIC values against B. subtilis (256 µg/mL) and S. epidermidis (512 µg/mL) with significant inhibitory activity. Methanolic extract of T. ponmudiana showed maximum inhibitory activity against S. epidermidis (19.2 mm) and S. aureus (15.8 mm). None of the microbial strain inhibited by petroleum ether extract. The methanolic extract of T. ponmudiana has been effective against S. epidermidis (256 µg/mL) and S. aureus (512µg/mL). Conclusion: The present result revealed that the methanolic extract of both the plants possesses significant antimicrobial activity when compared to petroleum ether extract.

Cite this article as: Kannaiyan Moorthy, Thambidurai Punitha, Raja Vinodhini , Suresh Mickymaray, Abraham Shonga, Zewdneh Tomass, Nooruddin Thajuddin4,Efficacy of different solvent extracts of Aristolochia krisagathra and Thottea ponmudiana for potential antimicrobial activity,Journal of Pharmacy Research 2015,9(1),35-40

Journal: Journal of Pharmacy Research , Volume: 9, Issue: 1.
Article Id: JPRS-QA-000081
Title: Development and validation of an UV spectrophotometric method for simultaneous determination of chlorthalidone and losartan potassium
Category: Quality Assurance
Section: Research Article
Country: India
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A new, simple, rapid and novel spectrophotometric method has been developed for simultaneous estimation of Chlorthalidone (CLR) and Losartan Potassium (LOS). The combination is estimated by AUC method . It involved measurement of area under curve in the wavelength range 280-290.5 and 235-245 for Chlorthalidone and Losartan Potassium respectively. Beer’s law obeyed in concentration range of 2-10 μg/ ml and 4-20 μg/ ml for Chlorthalidone and Losartan Potassium respectively. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific. It involves no heating and no organic solvent extraction. These method was validated for precision, reproducibility, linearity and accuracy as per ICH guidelines.

Cite this article as: Varsha M. Bhanushali, Madhuri A. Hinge, Snehal N. Patel,Development and validation of an UV spectrophotometric method for simultaneous determination of chlorthalidone and losartan potassium,Journal of Pharmacy Research 2015,9(1),54-59.