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Background: Mycobacterium bovis linked to cause bovine tuberculosis has been investigated and functionally annotated for several hypothetical proteins’ ability of enzyme activity as per the conserved domain present. Methods: As M. bovis genome codes in total 25% hypothetical proteins, we used CDD BLAST, INTERPRSCAN, PFAM and CATH domain search programs to find the enzyme functionality in these proteins. Results and discussion: Programs reported 347 hypothetical proteins posses enzymatic activity as per the enzyme domain conserved in the sequences at variable confidence levels. Conclusion: These proteins could be the markers, to investigate about their fate in the metabolism of M. bovis.

Cite this article as: P S Kewate, R C Urade, D G Gore, M A Soni and A P Kopulwar,In silico enzyme function prediction in hypothetical proteins of Mycobacterium bovis AF2122/97,Journal of Pharmacy Research 2015,9(3),182-189.

Steam distillation, Petroleum ether and ethanol extracts from Curcuma amada rhizomes were evaluated for there antimicrobial activity in-vitro. Ethanol extract exhibited broad-spectrum antibacterial and antifungal activity against the tested organisms. It showed highest antifungal and antibacterial activity against Aspergillus niger and Bacillus subtilis,  respectively. Activity indices of A. niger against miconazole (25 μg/ml) and B. subtilis against chlormphenicol (10 μg/ml) were 0.60 and 0.73, respectively.

Introduction:Rheumatoid Arthritis (RA) is a chronic disorder associated with substantial morbidity and mortality. Patients with active RA suffer from significant decline in functional capacity and many become work disabled within five years, from onset of symptoms despite treatment. Measureable outcome of these can be done with the help of Health Related Quality of Life (HRQoL) questionnaires. There is a need to study the HRQoL in RA patients, as very less data is available from Indian sub continent. Objective: To study of health related quality of life in rheumatoid arthritis patients. Materials and Methods: This cross-sectional observational study was conducted over a period of six months in Rheumatology unit of Medicine Department at JSS Hospital, Mysore, approved by the Institutional Human Ethics Committee. Health Assessment Questionnaire (HAQ) and Disease Activity Score (DAS) 28 were administered to assess the HRQoL in RA patients. Morisky’s Medication Adherence Scale (MMA) was used to assess the medication adherence. The correlation between the results of the questionnaires was analyzed using Pearson Correlation Coefficient method. Result:Total 105 patients who met the study criteria were enrolled. A statistically significant (P<0.05) correlation between the HAQ, MMA and DAS questionnaires was observed in the study. The early RA patients’ had a ‘mild to moderate’ disease activity with ‘good to fine’ mental health. The old RA patients had ‘moderate to severe’ disease activity with ‘fine to bad’ mental health. Conclusion :The patients with high MMA score had a better HRQoL and showed moderate decrease in the DAS and HAQ score.

Cite this article as: Sri Preethy N N, Shobha Churi, Subramanian R, Juny Joseph, Tejaswini G, Rakshitha KR, Rovin M Theempalangad, Study of health related quality of life in rheumatoid arthritis patients,Journal of Pharmacy Research 2015,9(3),194-200.

The aim of present study to evaluate antidiabetic activity of ethanolic extract of Bridelia scandens (Roxb) Wild. (Family: Euphorbiaceae) leaf in streptozotocin induced diabetic rats. The alcoholic extract of Bridelia scandens (Roxb) Wild was studied for antidiabetic activity in streptozotocin induced diabetic rats by oral administration of extract 200&400mg/kg body weight for the period of 28 days. The preliminary phytochemical study showed the presence of flavonoids, carbohydrates and glycosides, phenolic compounds and tannins. From the toxicity study it was observed that ethanolic extract of Bridelia scandens (Roxb) Wild was nontoxic up to the dose of 2000mg/kg body weight. The determination of blood glucose level by GOD-POD kit method. The effect was compared with oral dose of 0.6mg/kg Glibenclamide. The result shows the alcoholic extract of Bridelia scandens (Roxb) Wild level significantly lowered the blood glucose of hyperglycemic rats in the dose dependent manner and it was also comparable to that of the standard drug glibenclamide.

Cite this article as: V. Gomathi, B. Jaykar,Antidiabetic activity of ethanolic leaf extract of Bridelia scandens in streptozotocin induced diabetic rats,Journal of Pharmacy Research 2015,9(3),190-193

Floating matrix tablets are designed to prolong the gastric residence time after oral administration, at a particular site and controlling the release of drug especially useful for achieving controlled plasma level as well as improving bioavailability. With this objective, controlled release floating tablets of clarithromycin were formulated by using D-optimal mixture design for the treatment of Helicobacter pylori. The amount of HPMC K4M: HPMC K100LV in 1:1 ratio (X1), amount of Na- CMC (X2) and amount of sodium bicarbonate (X3) were selected as independent variables and percentage drug release at 3h (Y1), percentage drug release at 6h(Y2), percentage drug release at 12h (Y3) and total floating lag time (Y4) as dependent variables. Tablets containing HPMC K4M and HPMC K100LV, drug and different additives were prepared by wet granulation. The tablet composition and mechanical strength have great influence on the floating properties and drug release. Optimization of the evaluating parameters with Design expert software was employed to generate optimized formulation and criteria of desirability was employed. The optimized formulation was obtained with HPMC K4M: HPMC K100LV in 1:1 ratio (125.428 mg), Na-CMC (40.256 mg) and sodium bicarbonate (34.316 mg). The in vitro release data showed that the drug release follows zero-order release kinetics.