JPR:BioMedRx:An International Journal
ISSN NO: 2321-4988
BioMedRx journal is devoted to the promotion of Bio, Pharma and related disciplines (including medical, Nursing,Paramedical, Clinical   fields).It seeks to foster multi-disciplinary research and collaboration among scientists, pharmaceutical industries and healthcare sector as well as provide a National and international forum for the communication and evaluation of data, methods and opinions in pharmaceutical sciences and related disciplines.The Editor  welcome contributions of field relevance. Although primarily devoted to original research papers, the journal particularly welcomes reviews on current topics of special interest and relevance.

Manuscripts Published

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-MD-0000947
Title: Studies on biodiversity of Actinobacteria isolated from Mangroves of Vellappallam, Tamilnadu part of Bay of Bengal, india.
Category: Marine Drug
Section: Research Article
Country: India
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Marine environment is a potential source of secondary metabolites which provides an encouraging source for development of novel natural pharmaceuticals. Among the marine organisms, actinomycetes are a group of bacteria that are widely distributed and are known to play a very supporting role in the degradation of organic matter. The mangrove soil samples were collected from various locations in Vellappallam, and around Vedharanyam,[near Point Calimere, Lat. 10_ 18’ N and Long. 79_ 51’ E (seashore)], at Nagapattinam Dt. Tamil Nadu India. Soil samples were collected from the study site at randomly during the study period and physicochemical characteristics of sediment soil in marine environment of Tamilnadu part of Bay of Bengal, India. Various stations at different parts of the marine sites were selected for sampling and the following physicochemical parameters were recorded at different seasonal intervals. Totally 35 strains were isolated from marine sediment samples of Tamilnadu part of Bay of Bengal, India. Among them, 35 isolates were morphologically distinct on the basis of colour of spore mass, melanin pigment, reverse side pigment, soluble pigment, aerial and substrate mycelium formation and sporophore morphology. 35 isolates were identified as genus Streptomyces, (5) Actinopolyspora (10), Actinomadura (5), Nocardiopsis (7), Micromonospora (8) and Actinomyces (5).

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-PDRS-0000369
Title: Evaluation of effect of methanolic extract of Stereospermum suaveolens in the asthmatic animal.
Category: Plant Drugs Related Study
Section: Research Article
Country: India
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Bronchial asthma is an inflammatory disorder of the airways characterized by various airway obstruction, airway inflammation and bronchial hyper responsiveness. Air-way hyper-responsiveness (AHR) involves infiltration of leukocytes into the airway wall, which is associated with increased expression of cytokines, enzymes, and adhesion molecules within the airway. The present investigation was undertaken to evaluate the mast cell stabilizing and bronchial hyperactivity of methanolic extract of Stereospermum suaveolens Linn(MESS), bark in experimental animals. For evaluation of antiasthmatic activity of MESS was studied on broncho alveolar lavage fluid (BALF) in the ova albumin sensitized mice, Lung histopathology in mice and mast cell stabilizing activity was studied using compound 48/80 as degranulatimg agent in rat model. Treatment with MESS showed Significant decreased in the total leukocyte and differential leukocyte count in the BALF and serum IgE antibody count of the ova albumin sensitized mice was observed by administration of MESS (250, 500 and 750mg/kg, p.o., for 7 days,). Methanolic extract show dose dependently protected the mast cell disruption induced by compound 48/80 in rat model. The extract significantly result (*p<0.05, **p<0.01) for both hyperactivity airway response model in mice and mast cell protection in rat model. Antioxidant assay show the significant result for this plant extract. Based on overall result, protection of mast cell, inflammatory mediator level, lung histopathology and lung wet weight to dry weight ratio and lung wet weight to body weight ratio, it was found that MESS has not only mast cell stabilizing activity but also decreases bronchial hyperactivity by decreasing the infiltration of inflammatory cells in the airway and inhibition of release of histamine like mediators from the mast cell by stabilizing it.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-GM-0000368
Title: Prevalence and Antibiotic susceptibility pattern of bacterial agents causing Respiratory tract infection in Children
Category: General Medicine
Section: Editorial
Country: India
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Respiratory tract infection is the most common acute illness in pediatrics, ranging from self limited infection like common cold to life threatening infections like pneumonia and epiglottitis.The aim of the study is to detect the prevalence of bacterial etiology of respiratory tract infection in children and analyze antibiotic sensitivity pattern of the causative pathogens. This is a prospective study done in pediatrics department SRM medical college. Samples such as throat swab, sputum, pleural aspirate, blood etc were collected from children (age group from 6 months to 14 years old).Bacterial isolates were identified by routine biochemical reaction and antimicrobial susceptibility was done as per CLSI guidelines. Among 750 cases, 430 were upper respiratory tract infection and 320 were lower Respiratory tract infections. In upper respiratory tract infection, most common isolate was Group A Streptococcus 64(40%), followed by Group G Streptococcus 38( 24%), Group C Streptococcus 29(18%), Staphylococcus aureus18(12%), Moraxella catarrhalis7 (4%), Streptococcus pneumonia 1(1%).In lower respiratory tract infection, the most common was Streptococcus pneumonia 19(27%) and Klebsiella pneumoniae17(24%), followed by Staphylococcus aureus12(17%), Hemophilus influenzae5(7%), Escherichia coli7(10%), Group A Streptococcus 7( 10%). Most of the bacteria showed varied resistance to commonly used drugs.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-CADD-0000946
Title: Computer aided drug design(CADD) for rheumatoid arthritis using QSAR approach
Category: Computer Aided Drug Design
Section: Review Article
Country: India
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Quantitative Structure Activity Relationship (QSAR) study was performed for 74 compounds to test their inhibitory concentration activity(IC50) for 4,6-bis-anilino-1H-pyrrolo[2,3-d]pyrimidine derivatives for the disease Rheumatoid arthritis it has the important target of Insulin like growth factor 1 receptor(IGF-1R). The compounds are divided in to training set and test set using the leave 3 out method. Multiple linear regression analysis was done by calculating various molecular descriptors such as molecular volume, molecular mass, molecular refractivity, log P, kappa 1 index, K alpha 1 index etc. The (regression coefficient r = 0.843232) & ( r=0.711043) then cluster analysis was done for single linkage, group average & complete linkage method to compare which method is best to show maximum clusters. Using the software Tool for structure activity relationship (TSAR) 3.3 version is performed for QSAR study to prove which drug is best for rheumatoid arthritis disease

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-CADD-0000367
Title: A computational biology research –brain way to combat obesity through Melanocortin receptor
Category: Computer Aided Drug Design
Section: Editorial
Country: India
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Brain is a constant user of metabolic fuel, nurtured by other tissues. It is to be note worthy that this energy is used in the maintenance of transmembrane potential. Inhibition of certain hormone receptor in the brain has been shown to pose an effect the cholesterol metabolism and may be a way of protecting against cardiovascular diseases. Cholesterol level is maintained by balancing dietary cholesterol metabolism of lipoprotein in various tissues. Defective cholesterol regulation mainly to ischemia. This is due to elevated blood Low Density Lipoprotein cholesterol (LDL) whereas High Density Lipoprotein cholesterol in the blood is proven to be beneficial .Inhibiting the brains Melanocortin system by increasing the circulatory HDL or by blocking Melanocortin Receptor by performing Computer Aided Drug Design (CADD) would prove to be profitable And this “magic drug would combat obesity” faster and better.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-BT-0000366
Title: Oral health knowledge and behaviour among Life science Post graduates of Tirupati A Cross-sectional Study
Category: Biotechnology
Section: Research Article
Country: India
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Objective: Personal health practices have an important role in promotion of oral health of an individual. Dental diseases are irreversible, damage cannot be reverted. The knowledge among the people is of pivotal importance as these diseases cannot be identified at initial stages. Raising public awareness about dental check up may assist in early diagnosis. This study aims at finding out oral health knowledge and oral health behavior among Tirupati students. Methods: A structured Questionnaire was distributed to Post graduate students of Tirupati (N=190) during the academic year 2011-12. The respondents have filled in the forms in their class itself and ample time was given to respond. The questionnaire consisted information on general background, oral practices and oral health knowledge. Results: Oral health knowledge seemed to be very limited. More than half of the students didn’t visit a dentist for almost 2 years now. Only few knew the importance of fluorides in caries prevention. Very less proportion practiced dental flossing and used mouth washes. Only 10% of study group knew that a father could transmit S.mutans to the child. 68.42% knew that extraction is the not only possible treatment. Only 10.53% of the study group could attain a score of 5 but none could score the maximum points. Conclusion: It can be concluded that students had limited knowledge on oral hygiene and ideal oral hygiene practices. Their brushing practices are still far behind the international recommendation of twice a day. The establishment and strengthening of oral hygiene education, including the importance of tooth brushing for prevention of periodontal disease, has been proposed.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-PDRS-0000365
Title: In vivo anti-inflammatory and analgesic activities of leaf extracts from Kirganelia reticulata Baill.
Category: Plant Drugs Related Study
Section: Research Article
Country: India
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From the earliest times, herbs have been prized for their pain-relieving and healing abilities and today we still rely largely on the curative properties of plants. One such medicinal plant is Kirganelia reticulata Baill. belonging to family Euphorbiaceae, is a monoecious scandent shrub. Leaf is used for bleeding gums, diarrhoea in infants, burns, suppuration, chapped skin, small pox and syphilis. Inflammation is a local response of living mammalian tissues to injury. It is a pathophysiological response of living tissue to injuries that leads to local accumulation of plasmatic fluid and blood cells. The anti-inflammaroty activity of methanol and chloroform extracts were assessed using Carrageenan induced rat paw edema. The paw volume was measured at an interval of 30 mins up to 3 hrs by mercury displacement method using plethysmograph. 200 mg/kg b.w was taken as the therapeutic oral dose for the extracts. The analgesic effects of the plant extracts were evaluated using two models such as Acetic acid-induced writhing response and Formalin test in rats. Our methanol extract showed anti-inflammatory activity at various acute phases of inflammation and on formation of edema. The significant pain reduction from methanol extract might be due to the presence of analgesic principles acting with the prostaglandin pathways. The results obtained prove methanol extract to be potent anti-inflammatory and analgesic agents. It supports the traditional claim of the plant to be a source of drugs.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-PDRS-0000364
Title: Validation of silver nanoparticles isolated from roots of Plumbago zeylanica L. - An ethnomedicinal plant for skin diseases.
Category: Plant Drugs Related Study
Section: Research Article
Country: India
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Plumbago zeylanica L. multipurpose medicinal herb for the treatment of various skin diseases. Objectives: An attempt has been made to screen this important medicinal plant for secondary metabolites; and biogenesis of silver nanoparticles (SNPs) and test their antimicrobial efficacy. Materials and methods: Root extract of Plumbago zeylanica was prepared and screened for different secondary metabolites; this aqueous extract was subjected to AgNO3 to synthesize silver nanoparticles (SNPs), synthesis was confirmed by U.V-VIS spectroscopy and characterized by AFM; and toxicity of SNPs was tested against bacteria and fungi by paper disc diffusion method. Results and Discussion: The results indicate that the root of Plumbago zeylanica is source for different secondary metabolites like alkaloids, glycosides, flavonoids, phenols, saponins, steroids, tannins and triterpenoids. The SNPs formation from root extract was confirmed with the help of UV-VIS spectroscopy and characterized by AFM. The size of the SNPs was measured 40.9 nm with various shapes. The SNPs are having high toxic effect towards microorganisms than that of plant extract. Moreover the SNPs of Plumbago zeylanica root extract having high toxicity towards bacterial species than that of fungal species. Conclusion: The secondary metabolites of Plumbago zeylanica will be helpful to the phytochemists and pharmacologists for identification of new active principles and the environmentally benign SNPs can be used effectively against drug resistant bacterial and fungal strains.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-SDCS-0000363
Title: Synthesis and anti-inflammatory activity of substituted pyrimido[2,1-b][1,3]benzothiazole derivatives
Category: Synthetic Drug Chemistry study
Section: Research Article
Country: India
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A series of substituted pyrimido[2,1-b][1,3]benzothiazole derivatives were synthesized by reaction of 2-aminobenzothiazole, aromatic aldehyde and active methylene compound in ethanol through MCR mechanism. All the synthesized compounds were characterized by their physical and spectral data. All the synthesized compounds were screened for their in-vitro anti inflammatory activity by using inhibition of albumin denaturation technique. Most of the derivatives showed enhanced anti-inflammatory activity as compared to the standard drug diclofenac sodium. Compounds 1f, 1g, and 1i have shown the excellent anti-inflammatory activity with 78.57 %, 77.55 % and 75.51 % inhibition of albumin denaturation respectively.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-Pha-0000362
Title: Evaluation of anticancer activity of Andaman freeze dried powdered Morinda citrifolia L. fruit against Ehrlich Ascites Carcinoma (EAC) cell induced liquid and solid tumor in Swiss Albino mice.
Category: Pharmacology
Section: Research Article
Country: India
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Objective: The objective of the study was to establish the antitumor effect of powdered freeze dried Noni against Swiss albino mice bearing liquid tumor in peritoneal cavity and solid tumor in hind limb induced by Ehrlich ascites carcinoma (EAC) cell lines. Methods: For liquid tumor study Noni at doses 250 and 500 mg/kg body weight were administered to mice carrying EAC cell line up to 14 consecutive days. Half of the mice were sacrificed, for the assessment of antitumor activity by evaluating tumor parameters and hematological parameters and rest half were left for the survival studies by mean survival time (MST) and percent increase in life span (%ILS). For solid tumor study, tumor was induced by implanting EAC cell subcutaneously in the hind limb of mice. Same doses were used and mice were sacrificed after 21 days for evaluating tumor weight and tumor volume. Histopathological studies of tumor, liver and kidney were performed. Results: In liquid tumor, it was found that Noni significantly (p<0.01) decreases tumor volume, tumor weight, viable tumor cell count and increases the life span of mice bearing EAC cell lines. Hematological parameters gained their normal values. In solid tumor, Noni at both doses decreased tumor weight and Noni 500mg/kg decreased tumor volume significantly (p<0.05). Histopathological findings revealed that Noni showed less proliferation with some necrosis and apoptosis along with hepatoprotective and renoprotective action on liver and kidney. Conclusion: It was revealed from the experimental results that the powdered freeze dried Noni has potent antitumor activity.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: June
Article Id: JPRS-Pha-00001186
Title: Analgesic activity of methanolic extract of Fioria vitifolia (L.)
Category: Pharmacology
Section: Research Article
Country: India
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The plant Fioria vitifolia (L.) belongs to family “Malvaceae” and considering various medicinal properties of this plant. Wistar albino rats were used for studying analgesic activity of methonalic extract of Fioria vitifolia (L.) (MEFV) at doses 500 and 250 mg/kg p.o. Various models viz. Eddy’s hot plate and tail flick model followed for analgesic study.  In the hot plate model, the maximum effect was observed at 3hrs for both the doses of 500 and 250 mg/kg p.o., and in the tail flick method the effect was observed at 2hrs for both the doses of 500 and 250 mg/kg p.o., whereas in the hot plate and tail flick model, effect was comparable with standard drug of pentazocine (10mg/kg p.o). The extract showed marked analgesic activity in a dose dependent manner. Alcoholic extracts produced significant results in 500mg/kg p.o and 250 mg/kg p.o, the findings indicated the analgesic activity of the plant. The activity can be attributed to the phyto-constituents viz Alkaloids, Flavonoides, Tannins, Triterpenioid and Steroids present in the MEFV extract. It can be developed as a potent analgesic agent in future.