JPR:BioMedRx:An International Journal
ISSN NO: 2321-4988
BioMedRx journal is devoted to the promotion of Bio, Pharma and related disciplines (including medical, Nursing,Paramedical, Clinical   fields).It seeks to foster multi-disciplinary research and collaboration among scientists, pharmaceutical industries and healthcare sector as well as provide a National and international forum for the communication and evaluation of data, methods and opinions in pharmaceutical sciences and related disciplines.The Editor  welcome contributions of field relevance. Although primarily devoted to original research papers, the journal particularly welcomes reviews on current topics of special interest and relevance.

Manuscripts Published

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Ph-0000969
Title: Coprocessed Novel Superdisintegrants in Design and Evaluation of Rapimelts of an Antimigraine drug
Category: Pharmaceutics
Section: Research Article
Country: India
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In this current research work, Sumatriptan succinate Rapimelts were prepared using novel coprocessed super disintegrants like polacrillin potassium, crospovidone (in the ratios of 1:1 1:2 1:3) by solvent evaporation method. The developed Coprocessed mass was assigned to precompression parameters and a comparison was made with that of physical mixture of superdisintegrants. The developed mass shown favourable results compared to physical mixture. Tablets of Sumatriptan succinate were prepared by direct compression and subjected to post compression & evaluation parameters. The optimized disintegration time was found to be 10sec with CP2D formulation. Based on in vitro dispersion time (approximately 15sec) the promising formulation CP2D was tested for its drug release pattern using phosphate buffer PH 6.8 and shown drug release 98.2% in 10minutes following Non Fickian transport. FTIR spectral studies did not indicate any excipient incompatibility, either during mixing or after compression. Finally it was observed that directly compressible orally disintegrating tablets of Sumatriptan succinate with lower friability, acceptable taste, and shorter disintegration times were obtained using coprocessed polacrillin potassium, crospovidone at optimized concentration (1:2) along with Dicalcium phosphate. Thus aided in the faster release of drug thereby improve the patient compliance.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-SDCS-0000968
Title: Synthesis and antimicrobial screening of some novel substituted 4’-amino-1,3-diaryl-2-propen-1-one
Category: Synthetic Drug Chemistry study
Section: Research Article
Country: India
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Claisen-schmidt base catalyzed condensation reaction of 4’-amino acetophenone with different substituted benzaldehyde afford some substituted 4’-amino chalcones. The newly synthesized chalcones screened for antimicrobial activity. Most of these compounds showed potent activity.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-BM-0000967
Title: Investigation on isolation and identification of soil Mycoflora from terrestrial region on Melathiruppalakkudi, Thiruvarur District, Tamilnadu.
Category: Botany and Microbiology
Section: Research Article
Country: India
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Studies were carried out on the fungi present in four different seasons on the terrestrial region soil. Fungi isolation was done by the soil dilution method incubated at 28°C for 72 hours. The predominant fungal species were identified Viz., Aspergillus nidulans, A.tamarii A.versicolor A.wentii A sulphureus Cladosporium herbarum Geotrichum candidum Geotrichum sp. Gliocladiopsis sumatrensis Humicola sp. Penicillium janthinellum P. chrysogenum P.citrinum P.lanosum P.notatum Scopulariopsis sp. Sporotrichum sp. Trichoderma koeningii T.lignorum Torula sp. Trametes hirsuta Trichocladium achrasporum Trichoderma glaucum Trichoderma harzianum and Trichodochium sp. The physicochemical characteristics of soil samples were found to affect the distribution and population of fungi.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Ph-0000966
Title: Design and evaluation of sublingual immediate release films for treatment of osteoporosis
Category: Pharmaceutics
Section: Research Article
Country: India
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Raloxifene hydrochloride is recommended for treatment of osteoporosis. However, its oral bioavailability is 2 % due to extensive hepatic first pass metabolism and half-life is 27.7 hours after. Also nil solubility of drug makes it difficult for bioavailability achievement. Hence, it was envisaged to solubilize drug and developed by sublingual drug delivery system. Drug and polymers were characterized by FT-IR spectroscopy which found no interaction. For solubilization of drug in water, inclusion complex of raloxifene hydrochloride and β-cyclodextrin (1:2 and 1:4) were prepared and optimized by kneading method. Phase solubility studies revealed that, solubility of raloxifene hydrochloride was increased by complexation with β-CD and by addition of co-solvent ethanol and increase in pH. Sublingual immediate release films were prepared by solvent casting method by using inclusion complex of raloxifene (1:4), HPMC K4M, PVP K30, mannitol and xylitol. All formulation batches were evaluated for physicochemical parameters, mechanical studies, ex vivo mucoadhesive strength, ex vivo diffusion and in vitro drug release. Studies revealed that, with increase in HPMC K 4M concentration, tensile strength was increased whereas percent elongation and folding endurance were decreased whereas mucoadhesion strength was increased. Desired release was obtained in batch F2 with 94.71 ± 0.53 % in vitro release and 89.439 ± 0.426 % ex vivo permeation in 1 hour. Batch F2 (HPMC K4M and PVP K 30 in 3.5:1.5) exhibit best formulation. Stability studies on batch F2 revealed no significant reduction in drug content, in vitro dispersion time, ex vivo mucoadhesive strength and in vitro drug release.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Pha-0000965
Title: In vitro cytotoxicity activity of Gyrocarpus asiaticus bark extract
Category: Pharmacology
Section: Research Article
Country: India
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The aim of the study was to evaluate the cytotoxic activity of Gyrocarpus asiaticus. For evaluation HeLa and HCT6 cell lines were treated with the various concentrations of Methanolic extract of the bark. The result showed an IC50 value of 31.59µg/ml and 30.24µg/ml respectively in HeLa and HCT16 cell lines. It revealed that the plant had a potent cytotoxic activity against these cell lines. The cytotoxicity against other cell lines will be evaluated shortly.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-PDRS-0000964
Title: Phytochemical screening, total phenol content and antioxidant activity of different parts of Stachyterpheta urticifolia (Salisb) Sims
Category: Plant Drugs Related Study
Section: Research Article
Country: India
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Objective: To investigate the antioxidant activity of methanol, ethanol and acetone extracts of Stachyterpheta urticifolia (Salisb) Sims various parts and the determination of their total phenolic content as well as phytochemical screening. The extracts obtained from different parts were screened for possible antioxidant activities by free radical scavenging activity (DPPH) and FRAP methods. The amount of total phenols was analyzed with the Folin-Ciocalteau reagent. Results: Phytochemical analysis revealed the presence of saponins, flavanoids, terpenoids, steroids, glycosides, quinines, alkaloids, tannins and phenols in various extracts of root, stem, leaf and inflorescence. It was identified that the phenolic component is responsible for antioxidant activity. Methanol leaf extracts have more phenolic content when compared to ethanol leaf extracts and acetone leaf extracts. The methanol leaf extract have excellent antioxidant potential when comparative to ethanol leaf extracts and acetone leaf extracts. In DPPH free radical scavenging activity, the low IC50 value was showed by methanol leaf extract followed by ethanol leaf extracts as opposed to that of the scavenging effect of ascorbic acid. Conclusions: The study suggests that S. urticifolia methanol leaf extracts exhibit great potential for antioxidant activity and may be useful for their medicinal functions.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-PDRS-0000963
Title: Seasonal occurrence of endomycophytes from inner bark of Putranjiva roxburghii wall.
Category: Plant Drugs Related Study
Section: Research Article
Country: India
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Endophytic fungi from inner bark of Putranjiva roxburghii Wall were studied in three different seasons during 2009-2010. A total of 30 endophytes were recorded during rainy season, followed by 21 each in winter and summer season. Aspergillus niger, A. flavus, Bispora punctata., Curvularia lunata., Fusarium oxysporum and Verticillium sp.,were found to be dominant endophytes followed by Drechsclera sp., Nigrospora sp., Rhizophus stolonifer and Cladosporium species.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-PDRS-0000962
Title: Antimicrobial activity of Piper betle (L.) leaf extracts against pathogens of cereal crops
Category: Plant Drugs Related Study
Section: Research Article
Country: India
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Twenty phyto-pathogenic microbial strains were tested against chloroform and methanol crude extracts of Piper betle (Piperaceae) leaves by Agar well diffusion method. The extracts studied showed significant antimicrobial activity against all pathogens tested at a concentration of 5mg/ml dilution. It stands at 10ìg/ml of synthetic fungicide bavistin and 1mg/ml bacteriocides of chloramphenicol. This study proves that P. betle leaves have significant potential in the integrated pest management of common contaminants in storage grains and soil borne fungal pathogens of cereal crops. Further laboratory based studies prove that P.betle doesnot affect the seed germination in cereal crops.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Pha-0000961
Title: The therapeutic and regenerative effects of Periosteum in the treatment of gingival recession :A Systematic Review
Category: Pharmacology
Section: Research Article
Country: India
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Aim:The aim of this systematic review is to review the literature on regenerative effects of periosteum in the management of gingival recession. Materials and methods:An Electronic search through PUBMED upto 2013 and manual search was performed and a hand search of peer- reviewed journals of periodontology for relevant articles were performed. Studies included were randomized control trials, case series , case reports & animal studies with data on the regenerative effects of periosteum in treatment of gingival recession and its comparison with other techniques were included .Results :A total of 5 studies were included .The studies on gingival recession showed improvement in all parameters when compared to baseline values, 1 randomized control showed that periosteum is equally effective as Sub epithelial Connective Tissue Graft .

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Pha-0000960
Title: Additive effect of platelet rich fibrin in treatment of Intrabony defects- a systematic review and meta-analysis
Category: Pharmacology
Section: Research Article
Country: India
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Background: Over years various treatment modalities have been used in the management of periodontal intra bony defects effectively. Application of Autologous platelet concentrates has been widely applied to treat periodontal intra bony defects either solely or in combination with various other regenerative procedures. Aim: To systematically evaluate the additive effect of Platelet rich fibrin (PRF) in treatment of intra bony defects when used along with other regenerative procedures in terms of clinical and radiological outcomes. Material and methods: Randomized clinical trials evaluating the additive effect of Platelet rich fibrin (PRF) in treatment of intra bony defects were identified through screening in electronic databases and hand search in relevant journals. Reporting and methods were evaluated through an analysis of risk of bias and quality scores. Meta-analysis was also carried out for the variable of interest. Results: Four clinical trials were identified accomplishing inclusion criteria after full text reading and focused on evaluating additive effect of Platelet rich fibrin (PRF) when used with open flap debridement. Conclusion: Platelet rich fibrin(PRF) can prove to be effective as a sole regenerative material, for treatment of intra bony defects, in combination to open flap debridement.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Ph-0000959
Title: Formulation and evaluation of immediate release tablets of Carvedilol
Category: Pharmaceutics
Section: Research Article
Country: India
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The objective of the present study was to develop immediate release tablets of carvedilol in order to achieve rapid release in GIT which might result in enhanced absorption and thereby improved bioavailability. Six batches of solid dispersion were prepared using different ratios (1:4, 1:6 & 1:8) of hydrotropes (Nicotinamide & Urea) along with constant concentration of solubilizer (1% PEG). The best batch was selected to prepare immediate release tablets. The present worker prepared immediate release tablets comprising solid dispersion of carvedilol as main constituent in six preliminary batches using croscarmellose sodium, sodium starch glycolate and crospovidone as Superdisintegrants in different ratios along with other polymer and excipients. Secondary batches were prepared based on 32 factorial design with amount of Superdisintegrants (crospovidone) and hardness as two independent formulation variables. Two dependent response variables were considered i.e disintegration time and percentage invitro drug release. The desirability fraction revealed agreeable with experimental values. All the batches of immediate release tablets were evaluated with reference to different pre-compression and post-compression parameters The in-vitro drug release determination (97.759%) and results of accelerated stability testing revealed no physical and chemical changes in the tablets during three months studies.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Pha-0000958
Title: In vitro study of antioxidant and anticancer activities of new [4- Hydroxy- phenyl -4-{6-(acryloyloxy)hexyloxy}- benzoate] molecule
Category: Pharmacology
Section: Research Article
Country: India
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A new molecule of 4-hydroxy phenyl -4-{6-(acryloyloxy)hexyloxy}-benzoate was synthesized and screened for antioxidant activity.This new compound was synthesized through modifying the hydroxyl group of phenol basic nucleus and acryloyloxy and hexyloxy aliphatic groups was attached.In the present study the antioxidant and anticancer activities of new [4-hydroxy phenyl -4-{6-(acryloyloxy)hexyloxy}-benzoate molecule were studied.The antioxidant activity of new molecule was evaluated by 1,1-diphenyl-2-picryl hydrazyl free radical scavenging activity,scavenging of hydrogen peroxide,reducing power method,scavenging of nitric oxide and the calculated IC50 values for new molecule and reference standard Gallic acid were (141.4µg/ml,0.14µg/ml for DPPH method),(117.0µg/ml,5.60µg/ml for nitric oxide method).The cytotoxic studies indicate that the new molecule inhibit HeLa(cervical cancer) cell viability in a dose dependent manner.The IC50values calculated were >100µg/ml for new molecule,9.165µg/ml for standard drug cisplatin.These findings reveal that the new molecule 4-hydroxy phenyl -4-{6-(acryloyloxy)hexyloxy}-benzoate has good cytotoxic and antioxidant activities and could be an alternate candidate for the development of new biologically active compounds.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-SDCS-0000957
Title: Assay method development and validation for simultaneous estimation of amiloride hydrochloride and furosemide tablet by RP-HPLC
Category: Synthetic Drug Chemistry study
Section: Research Article
Country: India
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A simple, specific, precise, and accurate method development and validation of amiloride hydrochloride and furosemide tablet formulation by RP-HPLC was developed. The method employed Kromasil C18 (250×4.6mm,5ì) column as stationary phase. The mobile phase consisted of 0.005M. Hexane-1-Sulfonic acid sodium salt in water and methanol (50:50v/v) pH 4.0 by 1M acetic acid as eluent, at a flow rate of 1ml/min. Detection was carried out at wavelength 361nm. The retention time of amiloride hydrochloride and furosemide were found to be 5.2 and 6.9 minutes respectively. Linearity was established by over the concentration range of 2.5 to 7.5 ìg/ml for amiloride hydrochloride with correlation coefficients (r2) 0.9997 and 20 to 60 ìg/ml for furosemide with correlation coefficients (r2) 0.9992. The percentage recovery obtained for amiloride hydrochloride and furosemide were 99.42% and 99.67% respectively. The method has been validated as per ICH guideline and successfully applied to estimation of amiloride hydrochloride and furosemide in tablet dosage form.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-SDCS-0000956
Title: A Review On: Separation of different process related impurity in tablet formulation of drug
Category: Synthetic Drug Chemistry study
Section: Review Article
Country: India
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As new molecular entities are evaluated in clinical development the understanding, identification, quantification and control of impurities in drug substances are essential. As chemical processes used to produce drug substances mature from the early phases of development through registration, a concomitant maturing of process-related impurity understanding and control is required. Tablet formulations which are used in different diseases condition containing impurity as per ICH guidelines limit. This paper outlines the separation of different process related impurity in tablet formulation. All authors develop the analytical method for separation and increase resolution of different process related impurity with active pharmaceutical ingredient in tablet formulation.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Ph-0000955
Title: Formulation, Characterization and Evaluation of Self Emulsifying Drug Delivery System of Repaglinide
Category: Pharmaceutics
Section: Research Article
Country: India
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The oral route of drug administration is the most preferred route of delivery due to its ease of ingestion. But for much class II drugs it is inefficient mode of delivery due to limited drug absorption resulting in poor bioavailability. The objective of the present investigation was to formulate self emulsifying drug delivery systems (SEDDS) of Repaglinide to enhance its solubility and absorption. The solubility of Repaglinide was checked in different oils, surfactants, and co surfactants and ternary phase diagrams were constructed to evaluate the stable self emulsifying domain. The Repaglinide SEDDS were prepared using oleic acid (oil), Tween 80 (surfactant), and Transcutol HP (co surfactant). The particle size distribution, zeta potential, and polydispersity index were determined. The release of Repaglinide from SEDDS capsules was studied using USP dissolution apparatus I and compared the release from pure drug and marketed tablet (Novo norm). The in-vitro drug release studies of Repaglinide SEDDS formulation showed complete release in 60 min. The absorption of Repaglinide from SEDDS resulted in about 1.9 fold and 1.5 fold increase in bioavailability when compared with the pure drug and Novo norm respectively. This study confirmed that the SEDDS formulation can be used as a possible alternative to traditional oral formulations of Repaglinide to improve its bioavailability.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Pha-0000954
Title: Effect of Gymnema sylvestre on the pharmacokinetics and pharmacodynamics of metformin in diabetic rats
Category: Pharmacology
Section: Research Article
Country: India
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Gymnema sylvestre (GS) and Metformin both reducing insulin resistance. GS stimulate ß-cell function, increase ß-cell number, increases the enzyme activity responsible for glucose uptake and utilization. Metformin (MF), a biguanide increases glucose uptake and utilization in skeletal muscle and reduces hepatic glucose production. The effect of the combination of GS (100 mg/kg p.o and 500mg/kg p.o.) and MF (50 mg/kg and 100 mg/kg p.o.) on the pharmacokinetic parameters of MF was studied in STZ induced diabetic rats after single dose administration and multiple dosing for 15 days. Pharmacodynamic interactions were as well as studied by determing the effect of combination therapy on the serum glucose levels of STZ induced diabetic rats before and after multiple dosing for 14days. Histopathological studies were carried out by excising the pancreas after the treatment and the effect of combination on the volume of beta cells were compared to that of single (either MF alone or GS alone) administration and control. Pharmacokinetic studies revealed a decrease in the bioavailability of MF when given in combination with GS. The decrease in bioavailability was contributed by decrease in absorption rate constant and increase in clearance where as in the Pharmacodynamic study the combination in general showed decrease in serum glucose levels which was contributed by the hypoglycemic property of GS. But the combination did not decrease the serum glucose levels comparable to MF when given alone. Histopathological studies revealed that combination of GS with MF increased the volume of islets cells of pancreas. These observations show that Gymnema sylvestre is a potent hypoglycaemic but it contributes to a decrease in hypoglycemic effect of Metformin.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-PDRS-0000953
Title: Comparative Fluorescence analysis and Qualitative Phytochemical analysis of Citrus limettioides, Citrus pseudolimon of Nainital District
Category: Plant Drugs Related Study
Section: Research Article
Country: India
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Air dried leaves of Citrus limettioides and Citrus pseudolimon, were subjected Fluorescence analysis and Quantitative Phytochemical analysis. Fluorescence analysis was done in U.V. –Visible Chamber and Quantitative Phytochmeical was done by UV-Vis. Spectrophotometer (Shimadzu) Double beam. Citrus limettioides leaves shows that it is rich in Total phenolic content, Total flavonoids content, Total tannin content were up to a significance level, Citrus pseudolimon having rich Total starch residue and Total sugar content. Standard were uses as gallic acid, quercetin, and tannin acid for Total phenolic, Total flavonoids and Total tannin content. D- Glucose were used as a standard for Total starch residue and Total sugar Content.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-SDCS-0000952
Title: Synthesis and evaluation of new Indole derivatives for antimicrobial and anti-inflammatory activities
Category: Synthetic Drug Chemistry study
Section: Research Article
Country: India
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A series of indole derivatives were synthesised and profiled as antimicrobial, anti-inflammatory compounds. The compounds VIa, VIb and VIg were found to be relatively more effective against Gram positive organisms. Compouds VIa, VIb, VIg and VIi were more effective against Gram negative organisms. Compounds VIb,VIc and VId were found to be effective against C.albicans and A.niger organisms. Compounds VIb and VIe showed more potent anti-inflammatory activity.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-PDRS-0000951
Title: Formulation and evaluation of antimicrobial topical semisolid dosage form containing whole plant extract of Biophytum sensitivum
Category: Plant Drugs Related Study
Section: Research Article
Country: India
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Plants produce a diverse array of secondary metabolites, many of which possess antimicrobial activity in their biologically active forms or inactive precursors that are relatively safer than synthetic antibacterial compounds, offering profound therapeutic benefits and more affordable treatment (Trease et al. 1972) [1]. The beneficial medicinal effects typically result from the combinations of such secondary metabolites present in the plant to prevent or cure infectious conditions. One approach might be the testing of plants traditionally used for their antibacterial activities as potential sources for drug development. One of such folklore medicinal herb, Biophytum sensitivum (L) DC. (Oxalidaceae) is a small annual plant, growing throughout in wet lands (mostly plains) of tropical Africa, Asia and India; normally in the shade of trees and shrubs, in grasslands, open thickets, at low and medium altitudes and in the foothills of Himalayas. The parts of the plant has been known to possesses a wide spectrum of medicinal properties namely antiseptic properties, including positive effects in variety of skin infections, bruises, wounds & ulcer, burns & contusions, inflammatory diseases, and diabetes [2,3]. In this present study, the antibacterial potency of herbal creams and gels formulated with methanolic extract of Biophytum sensitivum was evaluated. The preliminary in vitro antibacterial activity of the dry methanolic extracts of dried whole plant of Biophytum sensitivum was determined against four different bacterial strains (Salmonella typhi, Staphylococcus aureus, Escherichia coli and Bacillus subtillis) using the agar well diffusion method to find the zone of inhibition of bacterial growth. Herbal creams and gels were prepared by incorporating the dry methanolic extract of whole plant of Biophytum sensitivum of into emulsifying cream and aqueous washable gel base and evaluated for their in vitro antibacterial efficacy. This study shows that Biophytum sensitivum has high potential as antibacterial agent when formulated as cream and gel for topical use and could therefore explain the successes claimed in the folklore use of the plant in the treatment of common skin conditions.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-PDRS-0000950
Title: Development and evaluation of a polyherbal topical formulation
Category: Plant Drugs Related Study
Section: Research Article
Country: India
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Ayurvedic literature finds mention of various plants used in skin care. Oil extracts of crude drugs are used in varied skin conditions and to nourish and revitalize the skin. However, oils for topical use are not aesthetically appealing. Hence, there is a need for a consumer friendly topical formulation containing oil extracts. In this study, an attempt has been made to investigate the therapeutic properties of a novel emulsion formulation. An oil extract containing Rubia cordifolia, Glycyrrhiza glabra and Symplocos racemosa was prepared . The mentioned plants have antiseptic, skin soothing and anti-inflammatory properties. The oil extract in this study forms the oil phase for the formulation of the emulsion system. Creams are a carrier system for oily extracts due to their numerous advantages like aesthetic appeal, better efficacy, stability and appearance. The formulation was evaluated for various stability indicating parameters, skin irritation and wound healing activity in vivo by excision wound healing model on rats. The formulations of herbal oil into an emulsion based system proved to be stable and non irritant. Formulation that contained 20% oil extract exhibited remarkable wound contraction proving its efficacy as a topical formulation beneficial in minor skin conditions.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Ph-0000949
Title: Development and validation of RP-HPLC method for simultaneous estimation of naproxen and paracetamol in their combined solid dosage form
Category: Pharmaceutics
Section: Research Article
Country: India
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A simple, precise and accurate RP-HPLC method has been developed and validated for the simultaneous estimation of Naproxen and Paracetamol available in tablet dosage form.The RP-HPLC method utilizes isocratic elution technique with C18 column (250 mm × 4.6 mm i.d., particle size 5 µm) with mobile phase consisting of Acetonitrile : Buffer (50:50 % v/v). The flow rate was 0.8 ml/ min and effluents were monitored at 240 nm. In HPLC method parameters validated were linearity 1.8-4.2 µg/ml and 3.0-7.0 µg/ml NAP and PCM respectively having correlation coefficient is 0.999. Accuracy for NAP and for PCM was found to be within the range and for precision %RSD was < 2 for both methods.

Journal: JPR:BioMedRx:An International Journal, Volume: 1, Issue: 7.
Article Id: JPRS-Ph-0000948
Title: Formulation and evaluation of Tropicamide nanoparticulate ophthalmic dosage forms
Category: Pharmaceutics
Section: Research Article
Country: India
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Formulation of a new tropicamide nanoparticulate (1%) ophthalmic suspension and gel was presumed to elicit a better contactibility of the drug at the site of action. These formulations were evaluated based on physiological, physicochemical and pharmacokinetic parameters. They were prepared aseptically keeping particle size 175 nm. Various additives i.e. viscosity enhancers, preservatives, chelating agent etc. were used to increase transient residence time including antimicrobial preservatives. Buffering agents with buffering capacity NMT 0.05% were used & pH adjusted at optimum stability. The finished products were subjected to stress conditions to check physical stability of the formulation. The prepared formulations i.e. nanoparticulate gel and suspension were found to be significantly efficacious in experimental animals. The gel formulation resulted in apparently significant in-vitro drug release (52.2% in 180 min.) comparative to the normal. The medriatic effects observed with nanoparticulate gel and suspension were found to be (15.5±0.67) mm and (13.2±0.31) mm respectively.