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The Journal of Pharmacy Research is an online Journal, publishing of correct version and document can be modified (when we/or author) get comments and any readers can give comments. The journal is devoted to the promotion of Pharmaceutical sciences and related disciplines (including Pharmacy, medical, Biotech, Botany, organic and medicinal chemistry, Nursing, Paramedical, prescription etc fields).
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Title: Development and Validation of a RP-HPLC Method for Simultaneous Densitometric Analysis ------
Authors: A.B. Mukherjee1, S. A. Gupta*1 and K. Kumar2------------------
Affiliations:1Asian University ,Department of Pharmaceutical Sciences, Indian College of Science, Pune – 411038,Maharastra, India. Address for corresponding author: A.B. Mukherjee Asian University ,Department of Pharmaceutical Sciences, Indian College of Science, Pune – 411038,Maharastra, India. E-mail: Phone:
INTRODUCTION G. acid is a group of triterpenoid saponins isolated from Gymnema sylvestre which is responsible for its anti-diabetic action1. G-------- basic hydrolysis2. -------------------ene3. 18β-Glycyrrhetinic acid is ----------------------- induced diabetic rats4. Extensive literature survey ------------t few HPTLC5, 6, 7 and HPLC2 methods have been reported for estimation of gymnemagenin. HPTLC8. 9, 10 and HPLC11, 12 methods have---------------------- compounds. To the best of our knowledge no reports were found for simultaneous estimation of gymnemagenin and 18β-glycyrrhetinic acid by HPTLC method. ------------------------- in UV regions5. It is therefore necessary to develop methods for rapid, precise ----------------- and inherent quality13, 14. HPTLC is most widely used at industrial level for routine analysis of herbal medicines15. Hence the objective of this work was to develop ------------------------ herbal drug formulation.
MATERIALS AND METHODS Solvents and chemicals Instrumentation and Chromatographic Conditions RESULTS AND DISCUSSION: HPTLC Method optimization ( table 1,2,3, and fig.1.2.3. ) legends of table and fig has to be given above the table and fig.
REFERENCES: for Journal:
1. Chatterjee SE, Shinha AA, Lankota A, Bpahed S, Gymnema Sylvestre: A Comprehensive Review, Drug Invention Today, 2010, 2,144-157. BOOK:
2.ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2 (R1), Nov. 2005.
3. ---- 40. ----------------------------------------------- once you will finish the preparation then submit the article
The Journal of Pharmacy Research
JPR seeks to foster multi-disciplinary research and collaboration among scientists, pharmaceutical industries and healthcare sector as well as provide a National and international forum for the communication and evaluation of data, methods and opinions in pharmaceutical sciences and related disciplines. The Editor welcome contributions of field relevance. Although primarily devoted to original research papers, the journal particularly welcomes reviews on current topics of special interest and relevance.
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Dr. B.L.R. Madhavi , [Area of Interest: Pharmaceutics] Dept. of Pharmaceutics ,Acharya & BM Reddy College of Pharmacy, (ABMRCP) Dr. Sarvepalli Radhakrishnan road, Soldevanvahalli, Bangalore –560090,Karnataka,India.
·Prof. Dr. G. Babu, M. Pharm., Ph. D.,Professor & Principal, [Area of Interest: Pharmaceutical Analysis, Natural Products Chemistry] Devaki Amma Memorial College of Pharmacy,Chelembra,Malappuram dist. 673634,Kerala,India.
·Dr. Suvardhan Kanchi, [Area of Interest: Analytical Chemistry, Biosensors, Green Synthesis of NPs, Dye Sensitized Solar cells] Department of Chemistry,Durban University of Technology,Durban,South Africa.
·Dr. Zhiyong Peng,Department of CardioVascular Sciences,Heart Institute,East Carolina University,115 Heart Dr,Greenville, NC 27834,USA.
·Dr. Debasish Bandyopadhyay [Area of Interest:Herbal Antioxidant Drug Study, Calcutta University, Kolkata.
·Dr. Ryszard Amarowicz , [ Area of Interest: Phenolic Compound, antioxidants, Chromatography, food analysis, bioactive compd of plant]Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland.
·Dr. Y. K . Gupta, Professor and Head,Dept. of Chemistry,B K Birla Institute of Engineering and Technology,CEERI road,Pilani (Rajasthan), India.
·Dr. Bishwambhar Mishra , (Assistant Professor, Dept. of Biotechnology, Sreenidhi Institute of Science and Technology,Hyderabad, India)
·Dr. Sat Pal Singh Bisht, ( Professor, Dept of Zoology, Kumaun University, Nainital-263002, Uttarakhand, India )
·Dr.Mohamed El Houseiny El Sebeay Shams,Head of the Department of Pharmacy Practice,Oman Pharmacy Institute,Ministry of Health, PO Box 1928, Muscat 114, Oman.
·Dr. Smaranika Pattnaik, School of Life Sciences, Sambalpur University, Burla 768019, Odisha,India.
·Dr. Kondawar M. S.Prof. and Head,Department of Quality Assurance,Appasaheb Birnale college of Pharmacy,South Shivajinagar, Sangli-Miraj road,Sangli - 416416 (MS)India.·Dr. Md. Amirul Islam , Pharmacy Discipline,Khulna University,Khulna-9208, Bangladesh·Prof. Vaithiamanithi Perumal,SAIMS Medical College, Indore,India·Dr. Biswa Mohan Sahoo,HOD & Associate Professor,Dept. of Medicinal Chemistry,Vikas College of Pharmacy,Putrela Road, Vissannapeta, Krishna Dist-521215 ,Andhra Pradesh, India·Dr. Popat Mohite.Associate Professor, Dapartment of Pharmaceutical Chemistry, MES's College of Pharmacy, SonaiTal- Newasa Dist-Ahmednagar, Maharashtra-414105·Dr.M.Swaroopa, UGC Post Doctoral Fellow in Biomedicine, Division of Zoology, Sri Padmavati Mahila Visvavidyalayam, Tirupati,A.P,India·Dr. Jiafeng Geng, School of Chemistry and Biochemistry, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA 30332, United States·Dr. M. V. Kumudhavalli., [Area of Interest: Phytochemistry and Pharmacology] Dept. of Pharmaceutical Analysis,Vinayaka Mission’s College of Pharmacy, Yercaud Main Road, Kondappanaikenpatty, Salem- 636008, T.N, India·Dr.Rajkumar Venkatadri [ area of Interest: Cancer Research, Pharmaceutical Sciences, Molecular Biology, Drug Invention], Department of Pharmaceutical Sciences, School of Pharmacy, Hampton University, 100 E Queen St, Hampt, VA 23668, USA·Dr. Pankaj Goyal, Amity Institute of Microbial Technology, Amity University, Noida, Sec-125, India·Dr. Bao Yang, PhD, Professor, [Area of Interest: Botany, enzyme ,bioactive compounds] South China Botanical Garden (Formerly South China Institute of Botany), Chinese Academy of Sciences, Guangzhou 510650, China.Dr. Appala Raju. Nemala, [Area of Interest: Pharmaceutical Analysis] , Department of Pharmaceutical Chemistry,8-2-249, Mount Pleasant, Road#3, Banjara Hills,Sultan-ul-Uloom College of Pharmacy, Hyderabad, Telanagana State. India
·Dr. Goutam Kumar Jana,Department of Pharmacognosy,Gayatri College of Pharmacy,At- Gayatri Vihar, PO- Jamadarpali, Via- Sason,Dist- Sambalpur, Pin- 768200, OrissaIndia
·Dr. Bhushankumar Suresh Sathe ,[Area of Interest: Pharmacy,Medicinal Chemistry], Department of Pharmaceutical Chemistry, MGSM’S Smt. Sharadchandrika Suresh Patil, College of Pharmacy,Chopda-425107, M.H., India
·Dr. Versha Parcha,[Area of Interest: Natural products and Drug Discovery] Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research,Balawala, Dehradun, Uttarakhand, India
·Dr.Tareeka Dilip Sonawane,[Area of Interest: Biotechnology and Bioinformatics (Reproductive toxicity, Molecular Biology)] D.Y.Patil University ,School of Biotechnology and Bioinformatics Level 5 , Sector 15, Plot No. 50 , CBD Belapur, Navi Mumbai 400 614,Maharashtra India
·Dr. Sabyasachi Maiti, [Area of Interest: Pharmaceutics (Novel Drug Delivery, Pharmacokinetics, Biopolymer Hydrogels)], Gupta College of Technological Sciences, Ashram More, GT Road, Asansol-713301, West Bengal, India
·Raghunath Singh,Senior Research Fellow (PhD Scholar), [Area of Interest: Nueropsychopharmacology, Ethnopharmacology, Inflammation, Neurodegenerative disorders] University Institute of Pharmaceutical Sciences, UGC-Center of Advanced Study (UGC-CAS),Panjab University,Chandigarh-160014,India
·Mohammed Rageeb Mohammed Usman, [Area of Interest: Pharmacognosy] ,Dept. of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda 425107, Dist - Jalgaon Maharshtra, India.
·Dr. Durgacharan Arun Bhagwat, [Area of Interest: Pharmaceutics (Development of Novel Drug Delivery System)], Dept of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Pin: 416 013, M.S. India.
·Dr.. Abhishek Laxminarayan Sharma, Q.A./ Q.C. Manager- Ayurvedic Section; Choksi Laboratories Limited; Vapi,India
·Dr. Agnimitra Dinda,[Area of Interest: Pharmaceutics ], Dept of Pharmaceutics, Gyana Jyothi College of Pharmacy,7-48/1, Gyana Jyothi Nagar,Uppal Bus Depot,Hyderabad-98,India
·Dr. A. Sai Ramesh, M.S. (by Research), Ph.D.,[Area of Interest: Medicinal Biochemistry, Natural Products, Medicinal Plants] Assistant Professor, Department of Biotechnology,Sree Sastha Institute of Engineering and Technology, Chemberambakkam, Chennai 600123, Tamil Nadu,India.
·Dr. Renu Sankar, Postdoctoral Researcher, [Area of Interest: Nanobiotechnology],Biomolecular Therapeutics Laboratory, Department of Chemistry, Sungkyunkwan University, Suwon, 440-746, South Korea.
Dr. Shashikant V. Bhandari.[Area of Interest: Drug design and development , Molecular modeling studies viz. QSAR, Anti-cancer], Professor, Head, Department of Pharmaceutical Chemistry, AISSMS College of pharmacy, Near RTO Office , Kenedy Road, Pune-4110001 ,M.H.,India.
Dr. Dhavalkumar V Patel, [Area of Interest: Pharmaceutics ] B K Mody Govt Pharmacy College,NR AJI DAM,Polytechnic Campus,Rajkot-360003, Gujarat,India.
·Mr. Mayank Sharma, Doctoral Research Fellow, [Area of Interest: Pharmaceutics(Drug Delivery, Nanotechnology) Department of Pharmaceutics, School of Pharmacy, Devi Ahilya Vishwavidyalaya, Takshashila Campus, Khandwa Road, Indore (M.P.) – 452001, India
Dr.Senthil Venkatachalam , Pharmaceutics [Area of Interest:Muco/Bioadhesive drug delivery system, Nanostructured Lipid carrier system], JSS College of Pharmacy, Rocklands, Ooty, Tamil Nadu 643001,India.
Dr. M.R Jayapal, [Area of Interest:Organic chemistry,Analytical chemistry,Nanotechnology]Dept of Chemistry, National University of Cordoba,Argentina.
Dr. Quan Wang,[ Area of Interest : fluorescence spectroscopy, single-molecule detection, biophysics, microfluidics, optics] Department of Chemistry, Stanford University, MC5080,USA.
·Dr. Fengguo Xu,Dept of Epidemiology and Public Health,Yong Loo Lin School of Medicine,National University of Singapore,MD3, 16 Medical Drive, Singapore 117600.
·Dr. D. Nagasamy Venketash,Dept of Pharmaceutics,JSS College of Pharmacy,Ooty - 643 001. Tamil Nadu. India.
·Dr. G.S. Lavekar, Former DG, CCRAS – AYUSH (Govt. of India)
·Dr. Debasish Bandyopadhyay ( Calcutta University, Kolkata)
·Dr. Vijay Dhondiram Wagh, Pharmaceutical Technology, India
·Dr. Ramdas Bhanudas Pandhare[Area of Interest: Pharmacology], University of Pune, India
·Dr. Faiyaz Ahamed, India
·Dr. Shete R.V, India
·Dr. Ramesh Putheti, United States
·Dr. Yuanxiong Deng, China
·Dr. Praveen Bansal, India
·Dr. C. Rajasekaran, India
·Dr. A. K. Meena, (NIAPR, Patiala) India
· Dr. Jongwha Chang, USA
· Dr. Somasekhar Penumajji, South Korea
·Dr. Geetha Kodali,Mexico
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Recent Manuscripts published
Title: Studies of the role of the methanol fraction of the ethanol layer of the chloroform-ethanol leaf extract of Dacryodes edulis on diclofenac-induced gastric ulcer in rats
Section: Research Article
Background and Aim: The leaves of Dacryodes edulis are used in traditional medicine for the treatment of gastric ulcer among other ailments in Nigeria. The aqueous extract, chloroform and ethanol layers of the chloroform-ethanol leaf extract of D. edulis were investigated for their qualitative and quantitative phytochemical compositions. Based on the outcomes of the preliminary investigations, the most promising of the three (ethanol layer) was further fractionated and the effects of the most desirable fraction (methanol fraction) on ulcer index, gastric juice volume, gastric juice pH and histopathology of diclofenac-ulcerated Wistar rats were determined. Methods: The qualitative and quantitative phytochemical compositions and acute toxicities of the aqueous extract, chloroform and ethanol layers as well as the effects of the methanol fraction of the ethanol layer of the chloroform-ethanol leaf extract of D. edulis on ulcer index, gastric juice volume, gastric juice pH and histopathology of diclofenac-ulcerated Wistar rats were determined using standard methods. Results:The qualitative and quantitative phytochemical analyses of the aqueous extract, chloroform and ethanol layers showed the presence and amounts of the following: alkaloids, flavonoids, tannins, saponins and steroids. Each of the aqueous extract, chloroform and ethanol layers was found to be safe at 5000 mg/kg body weight (b.w). At the tested doses (100, 200 and 400 mg/kg b.w), the methanol fraction caused significant (p < 0.05) and dose-dependent decreases in the ulcer indices and gastric juice volumes as well as increases in the gastric juice pH of the rats in the test groups compared with those of the rats in the ulcer-untreated group. Results of the histopathological evaluation supported the gastroprotective effects of the fraction. Results of the fraction were comparable with those of the standard anti-ulcer drug, ranitidine at the dose of 150 mg/kg b.w. Conclusion: Experimental findings indicate that the leaves of D. edulis possess remarkable anti-ulcer effect probably due to their phytochemical constituents.
Title: Diverse mechanisms of the anti-ulcer feature of the methanol fraction of the ethanol layer of the chloroform-ethanol extract of the leaves of Dacryodes edulis: Are anti-oxidant approaches and anti-lip
Section: Research Article
Category: Natural Drugs
Background and Aim: Investigations into medicinal plants with potent anti-oxidant activity will continue to garner the attention of researchers due to the contributory role of oxidative stress in a number of disease conditions and thus, the aqueous extract, chloroform and ethanol layers of the chloroform-ethanol extract of the leaves of Dacryodes edulis were evaluated for their anti-oxidant vitamin contents. Based on the outcomes of the preliminary investigations, the most promising of the three (ethanol layer) was further fractionated and the effects of the most desirable fraction (methanol fraction) on the activity of superoxide dismutase (SOD) and concentrations of reduced glutathione (GSH) and malondealdehyde (MDA) of diclofenac-ulcerated Wistar rats were determined. Methods: The anti-oxidant vitamin contents of the aqueous extract, chloroform and ethanol layers as well as the effects of the methanol fraction of the ethanol layer of the chloroform-ethanol extract of the leaves of D. edulis on the activity of SOD and concentrations of GSH and MDA of diclofenac-ulcerated Wistar rats were examined using standard methods. Results: The ethanol layer contained the highest concentration of vitamin C (0.79 ± 0.03 mg/100 g) while the chloroform layer contained the highest concentrations of vitamins A (1.83 ± 0.08 μg/g) and E (1.17 ± 0.07 mg/100 g). The aqueous extract contained the least concentrations of vitamins A (1.34 ± 0.06 μg/g) and E (0.86 ± 0.05 mg/100 g). At the administered doses (100, 200 and 400 mg/kg b.w), the methanol fraction caused significant (p < 0.05) and dose-related increases in SOD activity and GSH concentration as well as decrease in MDA concentration of the rats in the test groups compared with those of the rats in the ulcer-untreated group. Conclusion: This study reveals that the leaves of D. edulis possess remarkable anti-oxidant feature and anti-lipid peroxidation effect which may be crucial in the amelioration of gastric ulceration.
Title: In-vitro Antibacterial Activity of Sri Lankan Traditional Rice (Oryza sativa L.) Extracts against Bacteria Causing Skin and Soft Tissue Infections
Section: Research Article
Category: Natural Drugs
The aim of this study was to evaluate the potential antibacterial activity of the extracts of selected parboiled and un-parboiled Sri Lankan traditional rice against bacteria causing skin and soft tissue infections. Methanolic extracts of five Sri Lankan traditional rice including Kalu Heenati, Pokkali, Rathdal, Kahawanu and Sudu Murunga were used for in vitro antibacterial analysis. Antibacterial activity was evaluated in both the parboiled and un-parboiled rice samples. Concentrations of rice extracts used for the assays were 1000 µg/mL and 2000 µg/mL from the each extract. The antibacterial activity was evaluated against common bacteria causing skin and soft tissue infections (Staphylococcus aureus (ATCC 25923), Pseudomonas aeroginosa (ATCC 27853), Escherichia coli (ATCC 25922) and three clinical isolates of Methicillin resistant staphylococcus aureus (MRSA)) by well diffusion method and viable colony count technique. According to the results, methanolic extracts of all the selected Sri Lankan traditional rice varieties exhibited a potent antibacterial activity against Staphylococcus aureus with minimum bactericidal concentrations (MBC) of 200 µg/mL (minimum incubation time (MIT); 30 min) for Rathdal, 200 µg/mL (MIT; 60 min) for Kalu Heenati, Pokkali and Kahawanu, and 2000 µg/mL (MIT; 60 min) forSudu Murunga. The largest inhibition zones were observed in the extracts of Kalu Heenati and Rathdal. Kalu Heenati, Pokkali and Rathdal showed an efficacious inhibitory effect against MRSA (MBC; 200 µg/mL, MIT; 60 min), whereas the highest inhibitory activity was observed for Rathdal. Only the extract of Kalu Heenati was slightly active against Pseudomonas aeroginosa. None of the rice extracts studied showed an antibacterial activity against Escherichia coli.Reduction and loss of antibacterial activity was detected in rice after subjected to parboiling. In conclusion, Sri Lankan traditional rice varieties with red pericarp are good sources of antibacterial compounds mainly against Gram positive bacteria. Methanolic extract of Rathdal and Kalu Heenati showed a high efficacious inhibitory effect against skin and wound pathogens of Staphylococcus aureus and MRSA.