Article Id:JPRS-MB-00001069 Title:Phyto-chemical Screening and Antimicrobial Activities of Dhaman grass and Indian Ginseng Category:Microbiology Section:Research Article
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Abstract
Crude extracts of different parts (root, stem, leaf and seed) of Cenchrus ciliaris (CAZRI-358) and (Unripen fruit, ripen fruit and calyx) of Withania somnifera (RUBL-20668) were successively extracted with polar to non polar solvents using soxhlet assembly. The extracts were then screened for their Biological activity in-vitro against one gram positive bacteria (Staphylococcus aureus), two gram negative bacteria (Escherichia coli and Raoultella planticola) and one yeast (Candida albicans) by disc diffusion assay. Serial dilution method was used to determine minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). Water extract of Unripen fruit of W. somnifera showed highest antibacterial activity (IZ-13.33±0.24 mm, AI- 0.833) and highest antifungal activity (IZ-18.67±0.22 mm, AI- 2.667) against E. coli and C. albicans respectively as well as chloroform extract of stem (IZ-12.50±0.64 mm, AI- 1.042) and seeds (IZ-10.67±0.23 mm, AI- 0.821) of C. ciliaris against C. albicans and S. aureus.
Dr. Premlata Singariya*1, Dr. Padma Kumar1 and Dr. Krishan Kumar Mourya2
Affiliation(s) Name:
1. Laboratory of Tissue Culture and Secondary Metabolites, Department of Botany, University of Rajasthan, Jaipur- 302004,Rajasthan,India 2. Veterinary Officer & In-charge, Govt. Veterinary Hospital, Pahari (Bharatpur) Rajasthan,India
*Corresponding author. Dr. Premlata Singariya Dr. D.S. Kothari Post doctoral fellow, P. No. 55, Shri Kalyan Nagar, Kartarpura, Jaipur-302015 RAJASTHAN (INDIA)
Received on:20-09-2011; Revised on: 15-10-2011; Accepted on:10-12-2011