Journal: Journal of Pharmacy Research

Article Id: JPRS-ND-00001237
Title: Assessment of Variation in Rhoifolin Content in Aerial Parts of Uraria picta Desv. from Different Locations of Madhya Pradesh
Category: Natural Drugs
Section: Research Article
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    Background: Uraria picta Desv. is one of the ingredient of ten herbs formulation called Dashmoola. Rhoifolin is an active compound which has been isolated from the aerial parts of Uraria picta and has been established as marker compound for quality evaluation of this species. In the present time of global resurgence in traditional and alternative health care systems, the quality assurance of medicinal plants is very essential. Since, WHO and herbal Pharmacopoeia are also emphasizing on quality standardization of medicinal plants in terms of active ingredients, the present study was aimed to assess the variation in rhoifolin content in aerial parts of Uraria picta collected from eight locations of Madhya Pradesh for its quality standardization and selection of superior chemotype. Methods: The powdered samples of aerial parts were extracted by soxhlet extraction in methanol for 4 hours on water bath. Extracts were quantified for rhoifolin content using Ethyl acetate: Formic acid: Glacial acetic acid: Water (10: 1.1: 1.1: 2.6) mobile phase and a CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner, WIN CATS software Version 1.4.8 and Hamilton Syringe (100 µL). Results: Rhoifolin content varied from 0.039% to 0.195% among the aerial part samples of eight locations corresponding to seven agroclimatic regions of the state. The highest content was found in aerial parts of Pandado, Sehore (0.25%) followed by Kapoornala, Chhindwara (0.167%) and Pamakhedi, Khandwa (0.152%) respectively and lowest content in plant sample of Navali, Mandsaur (0.039%). Conclusions: The study provided significant variation in rhoifolin content in aerial parts of Uraria picta. The accession collected from Pandado, Sehore emerged out of best quality on the basis of rhoifolin content and superior chemotype too.

    Cite this article as:Hari Om Saxena, Brij Mohan and Arun Kakkar,Assessment of Variation in Rhoifolin Content in Aerial Parts of Uraria picta Desv. from Different Locations of Madhya Pradesh,Journal of Pharmacy Research 2016,10(5),185-190.

     

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    Author(s) Name:

    Hari Om Saxena*, Brij Mohan and Arun Kakkar

    Affiliation(s) Name:

    Natural Product Laboratory, Department of Chemistry, Govt. Model Science College, Pachpedi, Jabalpur (M.P.) – 482001, India

    *Corresponding author.
    Mr. Hari Om Saxena
    Non Wood Forest Produce Division,
    Tropical Forest Research Institute,
    P.O. – RFRC, Jabalpur (M.P.)

    Received on:12-01-2016; Revised on: 20-03-2015; Accepted on: 18-04-2016

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    Author:

    Hari Om Saxena*, Brij Mohan and Arun Kakkar

    Title:Assessment of Variation in Rhoifolin Content in Aerial Parts of Uraria picta Desv. from Different Locations of Madhya Pradesh
    Journal:Journal of Pharmacy Research
    Vol(issue):10 (May)
    Year:2016
    Page No: (185-190)
  • Experimental Methods Keywords

    Methodology:HPTLC ,Variation, Superior Chemotype
    Research Materials:Rhoifoiln, Uraria picta

Keywords

Rhoifoiln Aerial parts Uraria picta HPTLC Variation Superior Chemotype

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