Journal: Journal of Pharmacy Research
Title: RP-HPLC Method Development and Validation for Simultaneous Estimation of Amlodipine besylate,Valsartan and Hydrochlorothiazide in Tablet Dosage Form
Category: Pharmaceutics
Section: Research Article
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Abstract
A simple, specific, accurate and precise Reverse Phase High Performance Liquid Chromatographic method was developed for simultaneous estimation of Amlodipine Besylate (AB), Valsartan (VAT) and Hydrochlorothiazide (HTZ) in tablet dosage form on RP C-18 Column (Hypersil 250*4.6 mm) using Acetonitrile: Mixed Phosphate buffer (6.8 pH) (55:45) ( buffer was prepared by mixing the equal proportions of 0.01M Potassium dihydrogenphosphate and 0.001M Dipotassium hydrogenphosphate. pH was adjusted with Orthophosphoric acid) as mobile phase. The flow rate was 1.0 ml/min and effluent was monitored at 237nm. The retention time for VAT, HTZ and AB was found to be as 2.28, 2.99 and 4.57 respectively. Proposed method was validated for Precision, Accuracy, Linearity range, Robustness and Ruggedness.
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About the authors and Affiliations
Author(s) Name: Mohammad Younus1*, T. Karnaker Reddy1, Y. Ravindra Reddy1, Md. Fasiuddin Arif2
Affiliation(s) Name: 1. Bharat Institute of Technology (Pharmacy), Ibrahim Patnam, Rangareddy (Dist), A.P, India.
2. Alphamed Formulations Pvt.Ltd, Shamirpet, Rangareddy (Dist), A.P, India.*Corresponding author.
Mohammad Younus
Bharat Institute of Technology (Pharmacy), Ibrahim Patnam, Rangareddy
(Dist), A.P, India.
Received on: 15-06-2010; Revised on: 18-08-2010; Accepted on:13-09-2010-
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Author: Mohammad Younus1*, T. Karnaker Reddy1, Y. Ravindra Reddy1, Md. Fasiuddin Arif2
Title: RP-HPLC Method Development and Validation for Simultaneous Estimation of Amlodipine besylate,Valsartan and Hydrochlorothiazide in Tablet Dosage Form Journal: Journal of Pharmacy Research Vol(issue): 3 (November) Year: 2010 Page No: (2647-2650) -
Experimental Methods Keywords
Methodology: Reverse Phase High Performance Liquid Chromatography Research Materials: Amlodipine Besylate, Valsartan, Hydrochlorothiazide
Keywords
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