Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-00001449
Title: Simultaneous Estimation of Atenolol and Losartan Potassium By High Performance Liquid Chromatography and UV Spectrophotometric Method
Category: Pharmaceutics
Section: Research Article
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    A simple, rapid, precise and accurate isocratic reversed-phase HPLC method was developed and validated for the simultaneous determination of Atenolol (AT) and Losartan (LO) in commercial tablets. Chromatographic separation was achieved with ODS C-18 column (5ìm, 250 mm × 4.60 mm) using mobile phase consisting of acetonitrile : water : methanol (60:30:10) at a flow rate of 1 ml/min and UV detection at 235 nm. The retention time of Atenolol and losartan were observed at 2.18 min and 5 min respectively. The LOD for AT and LO were found to be 0.032 ìg/ml and 0.002 ìg/ml, respectively. In UV spectrophotometric method the lmax for Atenolol and Losartan was 275 nm and 232 nm respectively. The linearity of the proposed in UV spectrophotometric method was investigated in the range of 5-50 ìg/ml (r2 = 0.9942) for AT and (r2 = 0.995) for LO.

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    Author(s) Name:

     Dwivedi N. and Patil U. K.*

    Affiliation(s) Name:

    People’s Institute of Pharmacy and Research Centre,People’s University, Bhanpur, Bhopal (M.P.) 462037 India

    *Corresponding author.
    Patil U. K
    People’s Institute of Pharmacy and Research Centre,People’s University, Bhanpur,
    Bhopal (M.P.) 462037 India

    Received on:10-11-2011; Revised  on: 15-12-2011; Accepted on:12-01-2012

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    Author:

     Dwivedi N. and Patil U. K.*

    Title:Simultaneous Estimation of Atenolol and Losartan Potassium By High Performance Liquid Chromatography and UV Spectrophotometric Method
    Journal:Journal of Pharmacy Research
    Vol(issue):5 (January)
    Year:2012
    Page No: (681-685)
  • Experimental Methods Keywords

    Methodology:Reverse Phase HPLC; Simultaneous estimation
    Research Materials:Atenolol; Losartan

Keywords

Atenolol; Losartan; Reverse Phase HPLC; Simultaneous

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