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Abstract

Background: The present study was undertaken for qualitative, quantitative estimation of phytochemicals and standardization of Cipadessa baccifera leaf and to develop HPTLC finger print profile for its alcoholic extract.  Methodology: The shade dried leaves were subjected to analyse physico-chemical parameters as per standard procedures. The qualitative analysis and quantitative estimation of flavonoids, tannins and phenols were carried out by subjecting the specific extraction procedures using UV – Visible Spectrophotometer. TLC/HPTLC finger print profile was developed by using suitable solvent system. Results and discussion:  Physicochemical analysis of the leaves showed moisture content (9.39 %); ash content (4.58%) acid insoluble ash (0.61%); water soluble extractive value (18.63 %) and alcohol soluble extractive value (13.56%). The qualitative analysis of alcohol extract showed positive test for triterpenoid, flavonoid, alkaloid, coumarin, saponin, tannin and phenol. Quantitative estimation of the plant observed 1.90 % of tannin, 4.79 % of flavonoid and 2.48% of phenol.  Conclusion: HPTLC finger print profile was shown the presence of various phytochemical constituents. The present study on physicochemical analysis, qualitative and quantitative analysis revealed the presences of various biologically active constituents in this plant. This information can be utilised for authentication of this plant for the use of drug preparation.

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	JPR Solutions

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