Article Id:JPRS-P'Col-00001485 Title:Estra-1, 3, 5(10) - triene-3, 17 β-diol protects mitochondria against Cu-ascorbate induced oxidative damage in in vitro system: A novel therapeutic approach Category:Pharmacology Section:Research Article
Abstract
Audio Abstract
Authors
Pdf File
Citation
My Reference
Methodology
Abstract
Background: Cu-ascorbate is a well-established oxidative stress inducing agent in in vitro system. Terminalia arjuna is also a well known medicinal plant used as an anti-ischemic and cardiotonic agent for over three centuries in India. Estra-1, 3, 5(10) - triene-3, 17 β-diol (β-E) was identified as a component of the aqueous extract of bark of Terminalia arjuna. Aims and Objectives: To determine its antioxidant efficacy against cu-ascorbate induced oxidative stress in isolated goat liver mitochondria in an in vitro system. Methods: Goat liver mitochondria was incubated with Cu-ascorbate and different concentrations of β-E at pH 7.4 and 370C for 60 minutes. Then the reaction was stopped upon addition of EDTA. Enzymes and DNA from incubated mitochondria were isolated to determine the alteration in their activities and the status of the biomarkers of oxidative stress. Results:Incubation of goat liver mitochondria with Cu-ascorbate at pH 7.4 and 370C has resulted in significant elevation of lipid peroxidation, protein carbonylation , DNA damage, activities of Mn-superoxide dismutase, xanthine oxidase along with a concomitant decrease in reduced glutathione level, activities of the Kreb’s cyle and electron transport chain linked enzymes which is indicating towards the generation of reactive oxygen species (ROS) mediated mitochondrial dysfunction, that was confirmed by Janus green B staining. All of these changes were prevented from being occurring on co-incubation of mitochondria with β-E. Conclusion: From these above results it can be concluded that β-E possesses a significant antioxidant potential and provides protection to mitochondria against Cu-ascorbate induced oxidative damage.
1Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology, 92, APC Road, Kolkata 700 009, India. #Principal Investigator, Centre with Potential for Excellence in Particular Area (CPEPA), University of Calcutta, University College of Science and Technology, 92, APC Road, Kolkata 700 009, India 2Department of Physiology, Vidyasagar College, Kolkata 700 006, India 3 Cell biology and Physiology division, Indian Institute of Chemical Biology,4,S.C.Mullick Road, Kolkata 700032, India 4DBT-IPLS section,University Colleges of Science,Technology and Agriculture, 35,Ballygunge Circular Road, Kolkata 700019, India
*Corresponding author. Prof. Debasish Bandyopadhyay Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta University College of Science and Technology 92 APC Road, Kolkata 700 009, India
Received on:12-07-2016; Revised on: 19-08-2016; Accepted on: 23-09-2016