Journal: Journal of Pharmacy Research

Article Id: JPRS-P'Col-00001485
Title: Estra-1, 3, 5(10) - triene-3, 17 β-diol protects mitochondria against Cu-ascorbate induced oxidative damage in in vitro system: A novel therapeutic approach
Category: Pharmacology
Section: Research Article
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    Background: Cu-ascorbate is a well-established oxidative stress inducing agent in in vitro system. Terminalia arjuna is also a well known medicinal plant used as an anti-ischemic and cardiotonic agent for over three centuries in India. Estra-1, 3, 5(10) - triene-3, 17 β-diol (β-E) was identified as a component of the aqueous extract of bark of Terminalia arjuna. Aims and Objectives: To determine its antioxidant efficacy against cu-ascorbate induced oxidative stress in isolated goat liver mitochondria in an in vitro system. Methods: Goat liver mitochondria was incubated with Cu-ascorbate and different concentrations of β-E at pH 7.4 and 370C for 60 minutes. Then the reaction was stopped upon addition of EDTA. Enzymes and DNA from incubated mitochondria were isolated to determine the alteration in their activities and the status of the biomarkers of oxidative stress.  Results: Incubation of goat liver mitochondria with Cu-ascorbate at pH 7.4 and 370C has resulted in significant elevation of lipid peroxidation, protein carbonylation , DNA damage, activities of Mn-superoxide dismutase, xanthine oxidase along with a concomitant decrease in reduced glutathione level, activities of the Kreb’s cyle and electron transport chain linked enzymes which  is indicating towards the generation of reactive oxygen species (ROS) mediated mitochondrial dysfunction, that was confirmed by Janus green B staining. All of these changes were prevented from being occurring on co-incubation of mitochondria with β-E.  Conclusion: From these above results it can be concluded that β-E possesses a significant antioxidant potential and provides protection to mitochondria against Cu-ascorbate induced oxidative damage.

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    Author(s) Name:

    Arnab Kumar Ghosh1, Bharati Bhattacharjee1, Sanatan Mishra1,2,Nabanita Das3, Nirajan Ghosal1,Shamreen Naaz1, Arijit Pal4, Sib Sankar Roy3,Aindrila Chattopadhyay2, and Debasish Bandyopadhyay1#*

    Affiliation(s) Name:

    1Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology, 92, APC Road, Kolkata 700 009, India. #Principal Investigator, Centre with Potential for Excellence in Particular Area (CPEPA), University of Calcutta, University College of Science and Technology, 92, APC Road, Kolkata 700 009, India
    2Department of Physiology, Vidyasagar College, Kolkata 700 006, India
    3 Cell biology and Physiology division, Indian Institute of Chemical Biology,4,S.C.Mullick Road, Kolkata 700032, India
    4DBT-IPLS section,University Colleges of Science,Technology and Agriculture, 35,Ballygunge Circular Road, Kolkata 700019, India

    *Corresponding author.
    Prof. Debasish Bandyopadhyay
    Oxidative Stress and Free Radical Biology Laboratory,
    Department of Physiology, University of Calcutta
    University College of Science and Technology
    92 APC Road, Kolkata 700 009, India

    Received on:12-07-2016; Revised  on:  19-08-2016; Accepted on: 23-09-2016  

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    Author:

    Arnab Kumar Ghosh1, Bharati Bhattacharjee1, Sanatan Mishra1,2,Nabanita Das3, Nirajan Ghosal1,Shamreen Naaz1, Arijit Pal4, Sib Sankar Roy3,Aindrila Chattopadhyay2, and Debasish Bandyopadhyay1#*
    Title:Estra-1, 3, 5(10) - triene-3, 17 β-diol protects mitochondria against Cu-ascorbate induced oxidative damage in in vitro system: A novel therapeutic approach
    Journal:Journal of Pharmacy Research
    Vol(issue):10 (September)
    Year:2016
    Page No: (594-608)

  • Experimental Methods Keywords

    Methodology:Antioxidant efficacy, Oxidative stress, Mitochondria, Cytochrome C
    Research Materials:Estra-1, 3, 5(10)-triene-3,17β-diol , Terminalia arjuna

Keywords

Estra-1 3 5(10)-triene-3 17β-diol Antioxidant efficacy Oxidative stress Mitochondria Cytochrome C Terminalia arjuna

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