Journal: Journal of Pharmacy Research
Title: Phytochemical profiling and histochemical localization in leaf and stem of Trichosanthes cucumerina (L) var. cucumerina with reference to the influence of plant age and geographical variations
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
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Abstract
The study report comprises the results of an investigation on phytochemical evaluation, and histochemical localization in leaf and stem of medicinal species - T. cucumerina (L) var. cucumerina. This medicinal herb has wide application in folkloric medicine and Ayurveda owing to its antiinflammatory, antioxidant, hepatoprotective and antihelmintic potential. It is procured on a range of 35 -40 tonnes per year by various Ayurvedic pharmaceutical firms in Kerala for preparing herbal formulations. Parameters studied are the assessment of the efficacy of different extractant solvents in release of plant secondary metabolites, preliminary qualitative and quantitative analysis and evaluation of the occurrence and pattern of distibution of phytochemicals in tissues of leaf and stem through histochemical localization. The stem samples from two localities were compared for the influence of climatic and edaphic conditions on quantity of phytochemicals. Besides, these a comparison on stem of different maturity level (1 year and 2 years old) was also evaluated to assess the impact, if any, of the metabolic state of plant on quantity of secondary metabolites. Extraction of dried and powdered plant parts was carried out successively in five different solvents– petroleum ether, chloroform, ethyl acetate, methanol and water. The results of the study suggested that among the solvents, methanol and water were effective extractants in which most of the common secondary metabolites –alkaloids, flavonoids, phenolics, tannins, terpenoids and saponins – were released. Quantitative analysis of the methanolic and aqueous extracts concluded that both stem and leaf contain appreciable quantity (mg/g tissue) of alkaloids, phenolics and tannins in comparison to flavonoids and terpenoids. Studies on histochemical localization carried out using Wagner’s reagent concluded that alkaloids are mostly located in the parenchyma cells bordering the vascular bundles of stem and petiole. Flavonoids, localized with NaOH revealed that they were marked as a distinct yellow band in the sub hypodermal layer of stem. Tannin localization with FeCl3 also suggested their storage in parenchyma cells.
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About the authors and Affiliations
Author(s) Name: Arya Krishna U. K1 , Vinayan R2 and Sudha Bai R1*
Affiliation(s) Name: 1Post graduate and Research Department of Botany; University College; Trivandrum–34; Kerala; India
2 Research Unit – Kulathupuzha; Department of Forests and Wildlife; Government of Kerala; India*Corresponding author.
Dr. Sudha Bai R
Assistant Professor
Post Graduate & Research Department of Botany
University College
Trivandrum – 34, Kerala- IndiaReceived on:21-08-2016; Revised on: 12-09-2016; Accepted on: 17-10-2016
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Author: Arya Krishna U. K1 , Vinayan R2 and Sudha Bai R1*
Title: Phytochemical profiling and histochemical localization in leaf and stem of Trichosanthes cucumerina (L) var. cucumerina with reference to the influence of plant age and geographical variations Journal: Journal of Pharmacy Research Vol(issue): 10 (October) Year: 2016 Page No: (660-665) -
Experimental Methods Keywords
Methodology: phytochemicals, histochemical localization Research Materials: Trichosanthes cucumerina (L) var. cucumerina
Keywords
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