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Abstract

Background: A simple and rapid reversed phase-high performance liquid chromatographic method was developed for simultaneous determination of lidocaine, epinephrine and tetracaine in specific pharmaceutical children anaesthetic solution.  Method: The elution was done with a mobile phase gradient composed of methanol:phosphate buffer(v:v) on Chromosil C18 column (4 × 150 mm, 3 ìm particle size). The gradient elution started with 40% (V/V) methanol, ramped up linearly to 55% in 8 min, then kept constant until the end of the run. The mobile phase was pumped at a flow rate of 0.8 mL/min. The wavelength detector was set at 254 nm for lidocaine and tetracaine and 220 nm for epinephrine. Results and discussion: Retention times for lidocaine, epinephrine and tetracaine were around 3.058 min, 1.867 min and 6.917 min respectively. The reliability and analytical performance of the proposed HPLC procedure were statistically validated according to the respect of linearity, ranges, precision, accuracy, repeatibility, reproducibility, detection and quantification limits. Linear ranges were establish between 1,2-2,8mg/mL for lidocaine (r²=0,9973), 15-35μg/mL for epinephrine (r²= 0,9858) and 150-350μg/mL for tetracaine (r²=0,9968). Conclusion: This is the first time that a study combining simultaneous routinely determination of these three compounds has been performed in lidocaine, epinephrine and tetracaine paediatric anaesthetic solution.

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	JPR Solutions

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