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Abstract

Background: To establish the pharmacognostic parameters using pharmacopoeial standards for correct identity of whole plant of Epaltes pygmaea DC. (Asteraceae).  Methods: In pharmacognostic studies different types of evaluations were carried out that focus on taxonomy of the species, macro- and microscopic characters, physico-chemical parameters, fluorescence analysis, preliminary phytochemical screening and HPTLC finger print compared with marker compound stigmosterol. Results and discussion: The taxonomically it is a small annual herb, 8 to 20 cm high, minutely winged branched stem with aromatic roots, leaves are alternate, linear, lanceolate to oblong,  flower pink, solitary, terminal, heterogamous. Microscopically the plant showed the presence of bi or tri-radiate wings of the stem, leaves microscopy indicated the presence of anisocytic type of stomata, thin walled with small spindle shaped cells of leaf epidermis, powder microscopy showed vessel elements with short tail, vertical chain of elongated parenchyma cells with prominent simple pits, large, spherical and spiny pollen grains. Plant extracts showed the presence of flavonoids, steroids, phenols, tannin and sugars. The standard marker stigmosterol detected with Rf 0.54 in alcohol extract has been confirmed by TLC/HPTLC finger print. Conclusions: The study provides the scientific data for the proper identification and establishment of standards for the use of Epaltes pygmaea. These information will also be helpful to differentiate Epaltes pygmaea from closely related other species.

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	JPR Solutions

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