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Abstract

Objective: The present study was to investigate the antidiabetic, antioxidant, and antibacterial potentials of endophytic fungi isolated from the medicinal plant Cassia fistula. Methods: Endophytic fungi isolated from C. fistula by impregnation method. The fungal broth was extracted with various solvents like petroleum ether, dichloromethane, ethyl acetate, and butanol based increasing polarity. Antidiabetic efficacy of various extracts was determined by in vitro α-amylase and α-glucosidase assay. Furthermore, the extract was evaluated for antioxidant and antibacterial potentials using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay and well-diffusion method, respectively. Results: Six endophytic fungi (CFL1, CFL2, CFL3, CFL4, CFL5, and CFS1) were isolated from the plant C. fistula, and the different solvent extracts possess variation in phenol content which found ranged between 0.36 ± 0.005 and 43.18 ± 0.05 mg gallic acid equivalent/g. The α-amylase inhibition was found to be 17.83–60.86%, and also α-glucosidase enzyme inhibition was ranged from 10.04% to 56.76% against various fungal extracts tested. All fungal extracts possess significant antioxidant activity determined by scavenging potential of DPPH radical, and among all the extracts, dichloromethane of CFS1 was found maximum scavenging activity of 87.65%. The various fungal extracts showed significant bacterial inhibitions against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus. Conclusions: The present study reveals that the endophytic fungi of C. fistula could be the source of the potent lead drug against antidiabetic, antioxidant, and antimicrobial infections.

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	JPR Solutions

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