Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002902
Title: Genotoxigenic isolates of microorganisms detected in patients with colorectal cancer
Category: Pharmacology
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Aim: In our study, we spent molecular-genetic analysis of microbial cultures isolated from patients with colorectal cancer according to genetic determinants controlling adhesive, hemolytic (hlyA), and toxigenic activity. Methods: Genomic DNA extracted from samples of bacterial cultures, isolated from malignant tumor of the rectal mucosa as well as from normal mucosa colorectal of cancer patients was investigated using molecular genetic studies. Results: We found presence of colibactin (clb) and cytotoxic necrotizing factor 1 (CNF1) toxigenes using polymerase chain reaction and sequencing analysis of genetic determinants controlling an adhesive (papC, papH, afa, eae, and bfpA), hlyA , and toxigenic (lthB, sta, stx, clb, cnfI, cdtB, cif, and colE) activity of microbial cultures test samples. clb -producing microorganisms (Escherichia coli and Klebsiella pneumonia), isolated from the patients’ rectal mucosa, as well as E. coli, are known to be producents of cancer- causing toxin (CNF1). Conclusion: They also could be considered as etiologic agents of colorectal cancer, which is also desirable to be taken into account in the development of laboratory diagnostic and treatment-and-prophylactic measures.

  • Abstract Audio

    No Audio file found

  • About the authors and Affiliations

    Author(s) Name:

    Irina V. Rzhanova1 *, Ramil R. Vafin2 , TkhiNga Nguyen1 , Alexey I. Kolpakov1 , Olga N. Ilinskaya1

    Affiliation(s) Name:

    1Department of  Microbiology, Kazan (Volga region) Federal University, Ministry of Education and Science of the Russian Federation Kazan, Russia Kazan, Russia,

    2 Department of  Senior Researcher, Kazan Scientific Center of Russian Academy of Science, Kazan, 420088 Russian Federation, Russia,

    3 Department of Microbiology, Kazan Federal University, Employee of Medical University, Hanoi, Vietnam

    *Correspondent author: Irina V. Rzhanova, Kazan (Volga region) Federal University, Ministry of Education and Science of the Russian Federation Kazan, Russia Kazan, Russia.

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    0 View More
  • How to Cite my Article.

    Author:

    Irina V. Rzhanova1 *, Ramil R. Vafin2 , TkhiNga Nguyen1 , Alexey I. Kolpakov1 , Olga N. Ilinskaya1

    Title:Genotoxigenic isolates of microorganisms detected in patients with colorectal cancer
    Journal:Drug Invention Today
    Vol(issue):10 (December [ Special Issue 4 ])
    Year:2018
    Page No: (3479-3483)
  • Experimental Methods Keywords

    Methodology:Colibactin, Colicins, Colorectal cancer, Cytotoxic necrotizing factor-1, Escherichia coli, Klebsiella pneumonia, Polymerase chain reaction, Sequencing, Single nucleotide polymorphism
    Research Materials:Patients with colorectal cancer

Keywords

Colibactin Colicins Colorectal cancer Cytotoxic necrotizing factor-1 Escherichia coli Klebsiella pneumonia Polymerase chain reaction Sequencing Single nucleotide polymorphism

Our Services

Most Downloaded List