Logo

Abstract

Introduction: Hematoxylin is the most commonly used stain in histological laboratory. It is obtained from the heartwood of logwood tree, Haematoxylon campechianum. Durability, easy differentiation, and comparative permanency make it the most popular nuclear stain. However, there is apparent worldwide shortage of hematoxylin. Most commonly used counterstain with hematoxylin is eosin. Eosin is a synthetic xanthene dye. Although it is very efficient, it is hazardous to human and animal health and the environment. The worldwide shortage of hematoxylin and the hazardous effects of chemicals and synthetic dyes like eosin has led to the development of alternative organic and eco-friendly dyes from these natural sources. The use of these non-allergic, non-toxic, and biodegradable stains has become the need of the hour due to the increased environmental awareness. The aim of this study is to systematically review the use of various natural organic stains in histopathology. Materials and Methods: Comprehensive electronic and manual search of literature was done and 13 articles were included in the systematic review, based on the inclusion and exclusion criteria. The systematic review was done and the data extracted from the studies were tabulated. Results: The results suggested that aqueous and alcoholic extracts of various parts from the plant source can be used as alternative natural dyes, replacing routinely used hematoxylin and eosin stain in histopathology. Conclusion: Dyes from naturally occurring plant sources can be used as organic stains in histopathology.

Abstract Audio

No Audio file found

About the authors and Affiliations

	Name
	JPR Solutions

View Article File in pdf format.

Article File

View Article Citation Here.

How to Cite my Article.

Experimental Methods Keywords