Journal: Drug Invention Today

Article Id: JPRS-P col-00003153
Title: Organic stains used in histopathology - A systematic review
Category: Pharmaceutics
Section: Research Article
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    Introduction: Hematoxylin is the most commonly used stain in histological laboratory. It is obtained from the heartwood of logwood tree, Haematoxylon campechianum. Durability, easy differentiation, and comparative permanency make it the most popular nuclear stain. However, there is apparent worldwide shortage of hematoxylin. Most commonly used counterstain with hematoxylin is eosin. Eosin is a synthetic xanthene dye. Although it is very efficient, it is hazardous to human and animal health and the environment. The worldwide shortage of hematoxylin and the hazardous effects of chemicals and synthetic dyes like eosin has led to the development of alternative organic and eco-friendly dyes from these natural sources. The use of these non-allergic, non-toxic, and biodegradable stains has become the need of the hour due to the increased environmental awareness. The aim of this study is to systematically review the use of various natural organic stains in histopathology. Materials and Methods: Comprehensive electronic and manual search of literature was done and 13 articles were included in the systematic review, based on the inclusion and exclusion criteria. The systematic review was done and the data extracted from the studies were tabulated. Results: The results suggested that aqueous and alcoholic extracts of various parts from the plant source can be used as alternative natural dyes, replacing routinely used hematoxylin and eosin stain in histopathology. Conclusion: Dyes from naturally occurring plant sources can be used as organic stains in histopathology.

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    Author(s) Name:

    Rahul Mohandas*, Pratibha Ramani, Herald J. Sherlin, S. Gheena, Abilasha Ramasubramanian, K. R. Don, Gifrina Jayaraj, Archana Santhanam

    Affiliation(s) Name:

    Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, Chennai, Tamil Nadu, India


    *Corresponding author: Rahul Mohandas, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, 162, Poonamallee High Road, Chennai, Tamil Nadu, India.

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    Author:

    Rahul Mohandas*, Pratibha Ramani, Herald J. Sherlin, S. Gheena, Abilasha Ramasubramanian, K. R. Don, Gifrina Jayaraj, Archana Santhanam

    Title:Organic stains used in histopathology - A systematic review
    Journal:Drug Invention Today
    Vol(issue):11 (February)
    Year:2019
    Page No: (426-432)
  • Experimental Methods Keywords

    Methodology:Manual search of literature,13 articles
    Research Materials:Curcuma longa, Dyes, Extracts, Hibiscus sabdariffa,

Keywords

Curcuma longa Dyes Extracts Hibiscus sabdariffa Organic stains Plant sources

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