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Abstract

Objective: The aim of this study is to evaluate the antibacterial efficacy of 5.2% sodium hypochlorite (NaOcl) with sonic agitation devices. Materials and Methods: The protocol for the present study was approved by the Institutional Ethical Committee. 32 adult patients aged between 18 and 60 years, requiring root canal therapy in anterior and premolar teeth with non-vital pulp were selected for this study. The teeth with vital pulp, periapical cyst, calcified canals, and immature apices were excluded from the study. These teeth were analyzed by pre-operative radiographs and electric pulp test for monitoring their pulpal and periapical status. A detailed medical and dental history was taken. Patients were explained about the procedure, and written consent was taken before treatment. 20 permanent teeth were randomly divided into groups corresponding to the disinfection protocol. Local anesthesia (Lox 2%, Neon Laboratories Ltd., India) was administered, and isolation was done with rubber dam. The tooth and surrounding area were disinfected by swabbing with 5% iodine tincture. The root canal was accessed with size 10 K-file (Mani, Inc., Tochigi, Japan). Then, initial pretreatment root canal culture sample was taken with presterilized paper points. Two paper points were placed in the canal for 60 s and then transferred into two separate presterilized tubes of 2 ml brain heart infusion broth (marked as aerobic and anaerobic sample). This was designated as the first sample (S1). These tubes were transferred in 10 min for culturing under aerobic and anaerobic conditions. The working length was determined using electronic apex locator (Root ZX mini, JMorita Corp.) which was kept 0.5 mm short of the apex. This was confirmed by radiograph. Cleaning and shaping was done using conventional step-back technique. Master apical size was kept three sizes larger than the initial binding instrument. 5 ml of sterile water was used for irrigation during cleaning and shaping. Final sample (S2) was taken after agitating the sodium hypochlorite. Group 1 was agitated with EndoActivator, Group 2 was agitated with Waterpik Power Flosser, and Group 3 - positive control was not subjected to any agitation. Results: Canals agitated with EndoActivator and Waterpik Power Flosser showed antibacterial action against aerobic and anaerobic bacteria.

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	JPR Solutions

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