Journal: Drug Invention Today
Title: Determination of quality standards parameters of kemangi (Ocimum basilicum L.) leaves extract from three locations
Category: Drug Analysis
Section: Research Article
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Abstract
Objective: The objective of this study was to determine the parameters of the quality standard of kemangi (basil, Ocimum basilicum L.) leaves originating from three regions of Indonesia. Materials and Methods: The research method included collecting basil from three regions, namely Bandung, Cianjur, and Solo. The basil sample was then extracted using the maceration method followed by the phytochemical screening test. Determination of extract quality standardization parameters in accordance with the reference book parameters of Indonesian extract quality standards which included determination of moisture content, determination of ash content, determination of water-soluble ash content, determination of acid insoluble ash content, specific gravity, determination of water-soluble extractive content, and determination of ethanol soluble extractive content. Determination of essential oil contents using the thin-layer chromatography and gas chromatography/ mass spectrometry (GC-MS) methods. Results: The standardization parameter value of Bandung basil leaf extract was 9.5%, 4.68%, 82.86%, 1.47%, 0.82, 4.33%, and 64.33%, Cianjur basil leaf extract was 9.83%, 7.78%, 93.37%, 2.08%, 0.85, 22.33%, and 53.00%, and Solo basil leaf extract was 13.67%, 9.98%, 92.59%, 5.95%, 0.87, 41.67%, and 49.67%. Phytochemical screening of basil leaf extract showed that the metabolites contained were flavonoids, tannins, steroids, and saponins. The essential oil content found in basil leaves was 0.132%. GC-MS results in the three samples showed the linalool content in Bandung basil leaf extract was 2.62%, Cianjur 19.85%, and Solo 27.80%. Conclusion: It was found that there were differences in the quality standard parameters of each basil including the content of secondary metabolites and essential oils. Each region has a different standard of quality which cannot be generalized which was thought to be caused by differences in soil nutrient origin of plants.
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About the authors and Affiliations
Author(s) Name: Yoppi Iskandar1 , Resmi Mustarichie2 *
Affiliation(s) Name: 1 Department of Biology Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia,
2 Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
*Corresponding author: Resmi Mustarichie, Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia.
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Author: Yoppi Iskandar1 , Resmi Mustarichie2 *
Title: Determination of quality standards parameters of kemangi (Ocimum basilicum L.) leaves extract from three locations Journal: Drug Invention Today Vol(issue): 12 (February) Year: 2019 Page No: (374-379) -
Experimental Methods Keywords
Methodology: Gas chromatography-mass spectroscopy Research Materials: kemangi (basil, Ocimum basilicum L.)
Keywords
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