Journal: Drug Invention Today

Article Id: JPRS-BSN-00003309
Title: Polymerase chain reaction-based meat species discrimination
Category: Biomaterials ( Synthetic and Natural )
Section: Research Article
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    Background: Meat adulteration is a common industrial falsification practice worldwide. The problem with meat adulteration may be falsification is crime itself, healthcare related like food-allergy and associated with religious believes problem like those of Muslims and Christian. PCR-base assays considered the gold standard for highly sensitive, cheap and accurate assay over the another techniques. Mitochondrial DNA (mtDNA) is preferable over nuclear DNA (nDNA) due to that is it only maternally disseminated and so it is more stable and there is no or low recombination. One of the most commonly used mtDNA genes in species discrimination were 16s rDNA and NADH dehydrogenase genes. Materials and Methods: The study includes designing species specific-primer pairs and 20 blood sample (10 blood samples from cow and 10 blood samples from donkey) and 18 cow-donkey mixed blood samples were submitted to extract mtDNA. Monoplex and multiplex PCR were performed for cow and donkey mtDNA and mixed mtDNA respectively. Results: The results revealed that the 16s rDNA primer pair is specific for only cow and NADH dehydrogenase 4 gene is specific only for donkey and the monoplex PCR were positive for all cow (879 bp for 16s rDNA amplicon) and positive for all donkey (573 bp for NADH dehydrogenase 4 amplicon). The multiplex PCR results were positive for all 18 mixed samples (879 bp and 573 bp for 16s rDNA amplicon and NADH dehydrogenase 4 amplicon respectively). The results of sequencing confirm the results of PCR. Conclusions: The current study conclude the usefulness of designed species specific-primer pair in discrimination of meat authenticity even at 10% of mixed samples.

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    Author(s) Name:

    Khudhair A. N. Al-Ali, Hussein O. M. Al-Dahmoshi*

    Affiliation(s) Name:

    Department of Biology, College of Science, University of Babylon, Hilla, Iraq

    *Corresponding author: Dr. Hussein O. M. Al-Dahmoshi, Department of Biology, College of Science, University of Babylon, Hilla City, Nader 2, Iraq

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    Khudhair A. N. Al-Ali, Hussein O. M. Al-Dahmoshi*

    Title:Polymerase chain reaction-based meat species discrimination
    Journal:Drug Invention Today
    Vol(issue):12 (March)
    Page No: (577-585)
  • Experimental Methods Keywords

    Methodology:Meat adulteration
    Research Materials:Cow, Donkey, Meat


16s rDNA Cow Donkey Meat adulteration NADH dehydrogenase 4

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