Journal: Journal of Pharmacy Research

Article Id: JPRS-PB-00003328
Title: Deciphering the enzyme coding ability in Pseudomonas stutzeri ATCC 17588 hypothetical proteins by bioinformatics approach
Category: Pharmaceutical Biotechnology
Section: Research Article
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  • Abstract

    Objective: A study investigated Pseudomonas stutzeri hypothetical proteins for their ability to encode enzymatic function when predicted by conserved domain search and protein modeling. Materials and Methods: P. stutzeri encoding >1000 hypothetical proteins were searched for enzyme conserved domain at different confidence level using CDD-BLAST, Interproscan, PFAM, and CATH database. These enzyme domain positive hypothetical proteins were determined for structural features using PS2 protein structure prediction server and further confirmed at structural level for possible enzyme function. Results and Conclusion: The study put forward 55 hypothetical proteins encodes enzyme function of which 41 proteins successfully determined for structural features which was indicated that P. stutzeri hypothetical proteins surely encode enzyme features which need to investigate further in gene expression studies.

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    Author(s) Name:

    Yamini Meshram, Dilip Gore*

    Affiliation(s) Name:

    Department of Biotechnology, Sai Biosystems Private Limited Plot, Nagpur, Maharashtra, India

    *Corresponding author: Dilip Gore, Sai Biosystems Private Limited Plot 271 Raghuji Nagar, Nagpur – 440009, Maharashtra, India.

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    Author:

    Yamini Meshram, Dilip Gore*

    Title:Deciphering the enzyme coding ability in Pseudomonas stutzeri ATCC 17588 hypothetical proteins by bioinformatics approach
    Journal:Journal of Pharmacy Research
    Vol(issue):13 (March )
    Year:2019
    Page No: (30-35)
  • Experimental Methods Keywords

    Methodology:Enzyme domain, Homology modeling, Hypothetical protein
    Research Materials:Pseudomonas stutzeri

Keywords

Enzyme domain Homology modeling Hypothetical protein Pseudomonas stutzeri

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