Journal: Drug Invention Today

Article Id: JPRS-DA-00003364
Title: Determination of amylase activity in four selected grains (Ragi, Green Gram, Chickpea, and Fenugreek) at various germination stages
Category: Drug Analysis
Section: Research Article
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    Introduction: Grains and cereals worldwide are extremely important for both human and animal nutrition. More than plain grains, sprouted grains have more nutrition and energy while eating. Sprouts are rich in digestible energy, vitamins, minerals, amino acids, proteins, and phytochemicals, as these are necessary for a germinating plant to grow. Hence, from the current study, it was found that the sprouted grains show an increased enzyme activity which can be used for the maximum yield of enzymes in industries. Materials and Methods: The different grains used (Ragi, Green Gram, Fenugreek, and Chickpea) were collected from the local supermarket. All apparatus used was sterilized adequately with hot water at about 100°C to avoid infection and alteration of results. Results and Discussion: Amylase activity of chickpea and green gram showed increased enzyme activity on day 1. 4th day, fenugreek showed a greater increase in enzyme activity. On the final day, green gram showed a decrease in its graph while the other grains remained the same. From the research, it was found that the fermented grains show an increased enzyme activity on a particular day. Conclusion: This study exhibits that extraction done on a particular time of seed germination that can give a maximum enzyme yield.

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    Author(s) Name:

    S. Tasleem Abitha1 , R. Gayathri 2 *, V. Vishnu Priya2

    Affiliation(s) Name:

    1 Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Saveetha University, Chennai, Tamil Nadu, India,

    2 Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: R. Gayathri, Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Saveetha University, 160, Poonamallee High Road, Chennai – 600 077, Tamil Nadu, India.

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    Author:

    S. Tasleem Abitha1 , R. Gayathri 2 *, V. Vishnu Priya2

    Title:Determination of amylase activity in four selected grains (Ragi, Green Gram, Chickpea, and Fenugreek) at various germination stages
    Journal:Drug Invention Today
    Vol(issue):11 (April)
    Year:2019
    Page No: (925-927)
  • Experimental Methods Keywords

    Methodology: amylase activity
    Research Materials:Ragi, Green Gram, Chickpea, and Fenugreek

Keywords

Enzyme activity Grains Health benefits Industrial applications Sprouting

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