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Abstract

Background: Periodontal diseases are constantly associated with anaerobic bacterial species such as Porphyromonas gingivalis, actinobacillus actinomycetemcomitans in subgingival environment, and gingivitis and periodontitis are the common diseases which are the affecting the tissues surrounding the tooth. Bacteria are the prime etiological agent in periodontal disease, nearly 500 bacterial agent colonize the adult mouth, and anaerobic bacterial microorganism is known to cause destructive periodontal diseases. Mechanical removal of the biofilm and adjunctive use of antibiotics have been the conventional methods of periodontal therapy, and the emergence of ozone therapy in periodontal treatment seems to have promising future. Ozone is a triatomic molecule (O3 ), it has three oxygen atoms, and it is a gas with extremely pungent odor. It is an allotrope of oxygen that is much less stable than its diatomic allotrope O2 oxygen. Ozone gas has high oxidation potential and it is 1.5 times greater than chloride when used as an antimicrobial agent against bacteria, fungi, and protozoa; it can be used in the treatment of alveolitis as a replacement of antibiotic mouthwash for oral microflora. The objective of the study is to find the effect of ozone in the form of ozonated water used as an irrigating agent in scaling and root planning (SRP) procedure and finding the amount of bacterial microcolonies before and after SRP from the subgingival plaque samples comparing with distilled water as a control irrigating agent. Aim: The aim of the study is to assess the amount of microbial colonies formed on using ozonated water compared to distilled water as irrigating agent during SRP procedure and to know whether ozonated water is effective in bringing out changes in the number of microbial colonies under anaerobic environment. Objective: The objective of the study is to find the effect of ozone in the form of ozonated water used as an irrigating agent in SRP procedure and finding the amount of bacterial microcolonies before and after SRP from the subgingival plaque samples comparing with distilled water as a control irrigating agent. Materials and Methods: A randomized, double-blinded study was performed. A total of 20 patients suffering from chronic generalized periodontitis were recruited into the study; subgingival plaque samples were obtained from the patients before SRP and immediately after SRP. Both the patient and the clinician were blinded regarding the type of the irrigating agent used. The plaque samples were transferred into carrier medium and were cultured under anaerobic conditions; 24 h after culture, the samples were collected and total colony forming units were calculated. Results: On counting the number of colony forming units formed, it was found out that the amount of colonies formed in the ozone group had significantly lesser colonies formed when compared with the distilled water group from which we could interpret that ozonated water has better antibacterial properties. Conclusion: It is observed that there is drastic reduction in the bacterial microcolonies formed. It is clear that ozonated water is more effective in reduction of anaerobic organisms which are difficult to eradicate from the subgingival bacterial ecosystem henceforth ozonated water can be used as an alternative for water which is used commonly in daily practice.

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	JPR Solutions

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