Journal: Drug Invention Today

Article Id: JPRS-P col-00003401
Title: The effect of long-term treatment with an alpha-adrenergic receptor antagonist on contractility of smooth muscle, mRNA, calcium level, and protein kinase C alpha enzyme of prostate in men with beni
Category: Pharmaceutics
Section: Research Article
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    Aim: Find upregulation markers (i.e., mRNA expression, protein kinase C [PKC]-alpha enzyme level, prostate calcium level, and prostate contractility) in benign prostatic hyperplasia (BPH) patients with a history of long-term alpha-1 blocker (tamsulosin) therapy. Materials and Methods: Samples were taken from prostate chips in 30 BPH patients, which were divided into three groups: Without therapy, history of tamsulosin 0.4 mg therapy for 1 month, and >6 months, respectively. This study used organ baths to measure the prostate smooth muscle isometrical contractility and real-time polymerase chain reaction to count the number of mRNA α1-adrenergic receptors. Calcium levels were measured using confocal laser scanning; PKC-alpha enzymes were measured using ELISA. Results: Tamsulosin 0.4 mg therapy for 1 month decrease mRNA expression, PKC-alpha enzyme, prostate calcium level, and prostate contractility (significant in mRNA expression and PKC-alpha enzyme) compared to no therapy. Meanwhile, usage of tamsulosin 0.4 for >6 months significantly increased mRNA expression, PKC-alpha enzyme, prostate calcium level, and prostate contractility compared to therapy with tamsulosin 0.4 mg for a month. There were no significant differences in mRNA expression, PKC-alpha enzyme, prostate calcium level, and prostate contractility in the group using tamsulosin therapy for >6 months compared to the group without therapy. Conclusions: Tamsulosin 0.4 mg therapy for >6 months cause upregulation, shown by significant increases in mRNA expression, PKC-alpha enzyme, prostate calcium level, and prostate contractility.

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    Author(s) Name:

    Besut Daryanto1 *, Mulyohadi Ali 2 , Basuki Bambang Purnomo1 , Karyono Mintaroem3

    Affiliation(s) Name:

    1 Department of Urology, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia,

    2 Department of Pharmacology, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia,

    3 Department of Pathology and Anatomy, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia

    *Corresponding author: Besut Daryanto, Jalan Veteran, Lowokwaru, Malang 65145, East Java, Indonesia

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    Author:

    Besut Daryanto1 *, Mulyohadi Ali 2 , Basuki Bambang Purnomo1 , Karyono Mintaroem3

    Title:The effect of long-term treatment with an alpha-adrenergic receptor antagonist on contractility of smooth muscle, mRNA, calcium level, and protein kinase C alpha enzyme of prostate in men with beni
    Journal:Drug Invention Today
    Vol(issue):12 (April)
    Year:2019
    Page No: (663-669)
  • Experimental Methods Keywords

    Methodology:Calcium levels,confocal laser scanning;PKC-alpha enzymes; ELISA
    Research Materials: 30 Benign prostatic hyperplasia patients

Keywords

Alpha-1 blocker Benign prostatic hyperplasia Tamsulosin Upregulation

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